41 research outputs found
Inhibition of inducible nitric oxide synthase (iNOS) reduces multi-organ failure (MOF) in the thioacetamide (TAA) rat model
Low dose pentoxifylline (PTX) reduces mortality in an animal model of acute hepatic and multi-organ failure
Dimethyl-Sulfoxide Induces a Reduced Growth-Rate, Altered Cell Morphology and Increased Epidermal-Growth-Factor Binding in Hep G2 Cells
Culture of Hep G2 cells in medium containing 2 % (v/v) dimethyl sulphoxide (DMSO) resulted in a slowing of growth and a marked change in morphological appearance. By day 6, cultures containing DMSO had one-third the number of cells compared with parallel control cultures. Measurement of I-125-epidermal-growth-factor (EGF) binding to DMSO-treated cells revealed a striking time-dependent elevation in specific EGF binding to their cell surface. Increased binding was detectable within 24 h of the start of DMSO treatment, reaching, by 6 days, levels almost 25 times greater than those for control cells. Addition of EGF to DMSO-treated cells caused a rapid down-regulation of the EGF receptor, but did not alter their proliferation rate. Slowing of growth by other means, such as serum starvation, growth to confluence or culture in the presence of sodium butyrate, did not affect I-125-EGF binding, indicating a specific effect of DMSO on these cells