Dose T2-weighted short TI inversion recovery images on cardiac magnetic resonance reflect disease activity in cardiac involvement of sarcoidosis patients?

The 76th Annual Scientific Meeting of the Japanese Circulation Society 2012年03月18日 Fukuok

簡便な成熟ブタ膵内分泌細胞分離法の検討

膵細胞移植に際しては,大量かつ高純度の膵内分泌細胞を得ることが重要である.本研究では,リンパ球分離溶液を用いた簡便な成熟ブタ膵内分泌細胞の分離法を検討した.屠殺場より入手した成熟ブタ膵を細切・攪拌した後,細胞懸濁液を遠心分離し単離肝細胞を収集した.これをリンパ球分離溶液であモノポリ分離溶液(mono-poly resolving medium: MPRM)を用いて,膵内分泌細胞を外分泌細胞および血管内皮細胞,血液細胞などより分離・精製した.得られた膵分泌細胞数を計測し,形態学的・機能的観察を行った.その結果,(1)得られた膵内分泌細胞数:3.40±1.32×10^5/gのうち,ジチゾン染色陽性細胞数は2.81±1.09×10^5/gで,純度は82.6±2.5%であった.(2)免疫組織化学染色では60%がB細胞であった.(3)電顕では,典型的な分泌顆粒を有するB細胞,A細胞が認められた.(4)グルコース負荷試験では,分離直後のインスリン分泌能低下は1週間の培養で改善し,また40日間の培養中インスリンの分泌が保たれた.成熟ブタ膵内分泌細胞径は,ヒトリンパ球径に類似しており,MPRMを用いた1回の遠心操作で,膵分泌細胞は明瞭に分離され安定した細胞数が得られた.また,膵内分泌細胞の構成は膵島におけるそれと類似しており,超微細構造も保たれていた.以上より,MPRMを用いた膵内分泌細胞の分離法は,簡便で安定した細胞数が得られ,その形態・機能とも良好で有用な方法であると考えられた.Adult pig pancreatic endocrine cells were harvested by auto-digestion without added enzymes. The isolated, crude cells were purified by Mono-poly resolving medium (MPRM). The purity of the harvested cells was determined by dithizone staining and the number of pancreatic endocrine cells was counted. A large number of the cells were stained red with dithizone and showed high viability and a good insulin secretory response to glucose stimulation. The average number of cells purified by MPRM was 3.40 ± 1.32 × 10^5 cells/g pancreas and the number of dithizone-stained cells was 2.81 ± 1.09 × 10^5 cells/g pancreas. The insulin secretion from the pancreatic endocrine cells was maintained throughout a 40-day observation period and high glucose stimulation induced an increase in insulin secretion from the cultured cells. In the cells purified by MPRM, light and electron microscopic studies showed the cells to be typical pancreatic endocrine cells. The present purification method using MPRM allowed us to obtain quickly a large amount of adult pig pancreatic endocrine cells from the unpurified preparations. This is useful for transplantation and biochemical or biological studies of adult pig pancreatic endocrine cells

簡便な成熟ブタ膵内分泌細胞分離法の検討

膵細胞移植に際しては,大量かつ高純度の膵内分泌細胞を得ることが重要である.本研究では,リンパ球分離溶液を用いた簡便な成熟ブタ膵内分泌細胞の分離法を検討した.屠殺場より入手した成熟ブタ膵を細切・攪拌した後,細胞懸濁液を遠心分離し単離肝細胞を収集した.これをリンパ球分離溶液であモノポリ分離溶液(mono-poly resolving medium: MPRM)を用いて,膵内分泌細胞を外分泌細胞および血管内皮細胞,血液細胞などより分離・精製した.得られた膵分泌細胞数を計測し,形態学的・機能的観察を行った.その結果,(1)得られた膵内分泌細胞数:3.40±1.32×10^5/gのうち,ジチゾン染色陽性細胞数は2.81±1.09×10^5/gで,純度は82.6±2.5%であった.(2)免疫組織化学染色では60%がB細胞であった.(3)電顕では,典型的な分泌顆粒を有するB細胞,A細胞が認められた.(4)グルコース負荷試験では,分離直後のインスリン分泌能低下は1週間の培養で改善し,また40日間の培養中インスリンの分泌が保たれた.成熟ブタ膵内分泌細胞径は,ヒトリンパ球径に類似しており,MPRMを用いた1回の遠心操作で,膵分泌細胞は明瞭に分離され安定した細胞数が得られた.また,膵内分泌細胞の構成は膵島におけるそれと類似しており,超微細構造も保たれていた.以上より,MPRMを用いた膵内分泌細胞の分離法は,簡便で安定した細胞数が得られ,その形態・機能とも良好で有用な方法であると考えられた.Adult pig pancreatic endocrine cells were harvested by auto-digestion without added enzymes. The isolated, crude cells were purified by Mono-poly resolving medium (MPRM). The purity of the harvested cells was determined by dithizone staining and the number of pancreatic endocrine cells was counted. A large number of the cells were stained red with dithizone and showed high viability and a good insulin secretory response to glucose stimulation. The average number of cells purified by MPRM was 3.40 ± 1.32 × 10^5 cells/g pancreas and the number of dithizone-stained cells was 2.81 ± 1.09 × 10^5 cells/g pancreas. The insulin secretion from the pancreatic endocrine cells was maintained throughout a 40-day observation period and high glucose stimulation induced an increase in insulin secretion from the cultured cells. In the cells purified by MPRM, light and electron microscopic studies showed the cells to be typical pancreatic endocrine cells. The present purification method using MPRM allowed us to obtain quickly a large amount of adult pig pancreatic endocrine cells from the unpurified preparations. This is useful for transplantation and biochemical or biological studies of adult pig pancreatic endocrine cells

Paroxysmal atrial fibrillation recurrences and quality of life in symptomatic patients: A crossover study of flecainide and pilsicainide

Background: The therapeutic goals of atrial fibrillation (AF) patients are to reduce symptoms and prevent severe complications associated with AF. This study compared the efficacy of flecainide versus pilsicainide in reducing the frequency of AF and improving quality of life (QOL) in symptomatic paroxysmal AF patients without structural heart disease. Methods: The Atrial Fibrillation and Quality Of Life (AF-QOL) study was a prospective, multicenter, randomized, open-label crossover study that compared flecainide and pilsicainide as antiarrhythmic drug therapy. Patients were randomized to receive 3 months of treatment with flecainide twice daily or pilsicainide 3 times daily. Each treatment consisted of a dose-finding phase (weeks 1–4) and an efficacy phase (weeks 5–12). Forty-three patients completed the trial. The main outcome was the number of days with documented AF episodes using a patient-operated electrocardiogram. QOL questionnaires (SF-36 and AF-specific QOL scores) were also completed. Results: The median (range) AF frequencies (days/8 weeks) were 2 (0–50) in the flecainide treatment group and 1 (0–54) in the pilsicainide treatment group (no significant between-group difference). No significant difference in the first recurrence of AF during the efficacy phase was noted between flecainide and pilsicainide treatments. The frequency and severity scores of AF-related symptoms improved from baseline to the end of the treatment periods. No significant differences in SF-36 or AF-related QOL scores were noted between the treatment groups. Conclusions: This study found no difference in AF frequency or QOL between symptomatic paroxysmal AF patients who received flecainide or pilsicainide