Chlamydomonas reinhardtii Is a potential food supplement with the capacity to outperform chlorella and spirulina

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Chlamydomonas reinhardtii is a green microalgae used as a model organism associated with biotechnological applications, yet its nutritional value has not been assessed. This study investigates the nutritional capacity of C. reinhardtii as an additional value for this species beyond its known potential in biofuels and bio-products production. The composition of key nutrients in C. reinhardtii was compared with Chlorella and Spirulina, the species widely regarded as a superfood. The results revealed that the protein content of C. reinhardtii (46.9%) was comparable with that of Chlorella (45.3) and Spirulina (50.4%) on a dry weight basis. C. reinhardtii contained all the essential amino acids with good scores based on FAO/WHO values (0.9–1.9) as in Chlorella and Spirulina. Unsaturated fatty acids predominated the total fatty acids profile of C. reinhardtii were ~74 of which ~48% are n-3 fatty acids. Alpha-linolenic acid (ALA) content in C. reinhardtii (42.4%) was significantly higher than that of Chlorella (23.4) and Spirulina (0.12%). For minerals, Spirulina was rich in iron (3.73 mg/g DW) followed by Chlorella (1.34 mg/g DW) and C. reinhardtii (0.96 mg/g DW). C. reinhardtii, unlike the other two species, consisted of selenium (10 µg/g DW), and had a remarkably lower heavy metal load. Moreover, C. reinhardtii contained relatively high concentrations of chlorophyll (a + b) and total carotenoids (28.6 mg/g DW and 6.9 mg/g DW, respectively) compared with Chlorella (12.0 mg/g DW and 1.8 mg/g DW, respectively) and Spirulina (8.6 mg/g DW and 0.8 mg/g DW, respectively). This study confirms that, based on its nutrient credentials, C. reinhardtii has great potential as a new superfood or ingredient for a food supplement

Seasonal variation in the nutrient profile of Arthrospira fusiformis biomass harvested from an Ethiopian soda lake, Lake Chitu

The extent of seasonal variation in the nutrient profile of Arthrospira biomass harvested from Lake Chitu was investigated to evaluate the variability of the quality of the product over a period of a year. Protein content varied from 47.9 to 55.7% for wet season biomass samples and from 39.2 to 40.8% for dry season samples. Dry season samples were characterized by relatively higher carbohydrate values (38.0–41.3%). Higher proportion of amino acids and unsaturated fatty acids were recorded for biomass harvested in wet season. Similarly, higher contents of phytonutrients (pigments) were recorded for wet season biomass samples: chlorophyll a (8.2–10.3 mg g−1), phycobiliproteins (104.1–120.7 mg g−1), total carotenoids (3.17–4.31 mg g−1), and β-carotene (1.24–1.61 mg g−1). The contents of Na and K were higher for a dry season biomass whereas other major (Ca, P, Mg) and trace (Mn, Fe, Cu, Zn, Se) minerals were found relatively in higher quantities in a wet season biomass. The nutritional composition of Arthrospira from Lake Chitu was found to be relatively comparable to that found in commercial Arthrospira products in the market. The significance of the findings is discussed in relation to potential sustainable production of Arthrospira biomass from this lake

Intention, magnitude and factors associated with bottle feeding among mothers of 0–23 months old children in Holeta town, Central Ethiopia: a cross sectional study

BACKGROUND: World Health Organization (WHO) recommends that bottle feeding should be avoided for infant and young child feeding since it has an impact on optimal breastfeeding, appropriate complementary feeding and bottles with a nipple are prone to contamination. The objectives of this study were to determine intention, magnitude and factors associated with bottle feeding among mothers of 0–23 months infants and children. METHODS: Community based cross sectional study was conducted from February to May 2016. A total of 422 mothers who had children 0–23 months were included in the study. Systematic random sampling was used to select the study subjects. Data were collected using a pre-tested interviewer administered structured questionnaire. The data were cleaned, coded, entered in to EPI-INFO version 3.5.4, and transferred and analyzed using SPSS. Odds ratio was calculated with 95% CI to identify factors associated with bottle feeding practice. P-values less than 0.05 were considered as statistically significant. RESULTS: The prevalence of bottle-feeding in this study was 19.6% and another 27.6% mothers have intention of bottle feeding. Being infant age of 0–5 months [AOR = 0.27;95% CI:(0.12,0.62)] and being a housewife [AOR = 0.37;95% CI:(0.21,0.67)] were negatively associated while having three under five children [AOR = 2.77;95% CI:(1.07,7.14)], not attending PNC follow-up [AOR = 2.13;95% CI:(1.19,4.97)], lower age of mothers [AOR = 3.38;95% CI:(1.48,7.73)] and not counseled on bottle feeding [AOR = 2.18;95% CI:(1.24,3.83)] were positively associated with bottle feeding. CONCLUSION: The prevalence of bottle feeding in the study area was high compared to the national prevalence of bottle feeding. Working outside home, lower maternal age, older age of children, having more than one under five children in the household, not attending PNC follow-up and not counseled on bottle feeding were found to be risk factors associated with bottle feeding practice in the study area

Magnitude and determinants of appropriate complementary feeding practice among mothers of children age 6-23 months in Western Ethiopia.

BackgroundAppropriate complementary feeding has the potential to prevent 6 percent of all under five deaths particularly in developing countries. However, infant and young child feeding practices in Ethiopia are suboptimal. Data on complementary feeding practices in Horro district are also lacking. Therefore, this study aimed to determine magnitude and determinants of appropriate complementary feeding practice among mothers of children age 6-23 months in Horro district, Western Ethiopia.MethodsCommunity based cross sectional study was conducted from February to March 2016 in six kebeles of Horro district, Western Ethiopia. A total of 325 mothers of children age 6-23 months were included in the study. Data were collected using pre-tested interviewer administered structured questionnaire; entered into EPI-INFO version 3.5.4 and analyzed using SPSS version 20. Odds ratio was calculated with 95% CI to identify determinants of appropriate complementary feeding practice. P-values less than 0.05 were considered as statistically significant.ResultsThe magnitude of appropriate complementary feeding practice in the study area was 9.91%. Lower age of child (6-11months) [AOR: 0.195, 95% CI: (0.045-0.846)], having no formal education [AOR = 0.115:95% CI: (0.002, 0.290)] and giving birth for the first time [AOR = 0.271:95% CI: (0.011, 0.463)] were factors negatively associated with appropriate complementary feeding practice.ConclusionOnly one tenth of mothers practiced appropriate complementary feeding. This strongly calls for sustained nutrition education targeting uneducated mothers, mothers who gave birth for the first time and those with very young children to improve the practice

Nutritional and genetic variation in a core set of Ethiopian Tef (Eragrostis tef) varieties.

BACKGROUND: Tef (Eragrostis tef) is a tropical cereal domesticated and grown in the Ethiopian highlands, where it has been a staple food of Ethiopians for many centuries. Food insecurity and nutrient deficiencies are major problems in the country, so breeding for enhanced nutritional traits, such as Zn content, could help to alleviate problems with malnutrition. RESULTS: To understand the breeding potential of nutritional traits in tef a core set of 24 varieties were sequenced and their mineral content, levels of phytate and protein, as well as a number of nutritionally valuable phenolic compounds measured in grain. Significant variation in all these traits was found between varieties. Genome wide sequencing of the 24 tef varieties revealed 3,193,582 unique SNPs and 897,272 unique INDELs relative to the tef reference var. Dabbi. Sequence analysis of two key transporter families involved in the uptake and transport of Zn by the plant led to the identification of 32 Zinc Iron Permease (ZIP) transporters and 14 Heavy Metal Associated (HMA) transporters in tef. Further analysis identified numerous variants, of which 14.6% of EtZIP and 12.4% of EtHMA variants were non-synonymous changes. Analysis of a key enzyme in flavanol synthesis, flavonoid 3'-hydroxylase (F3'H), identified a T-G variant in the tef homologue Et_s3159-0.29-1.mrna1 that was associated with the differences observed in kaempferol glycoside and quercetin glycoside levels. CONCLUSION: Wide genetic and phenotypic variation was found in 24 Ethiopian tef varieties which would allow for breeding gains in many nutritional traits of importance to human health