20 research outputs found
Interpretation of multi-detector computed tomography images before dissection may allow detection of vascular anomalies: a postmortem study of anomalous origin of the right subclavian artery and the right vertebral artery
Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction
Useful DNA typing using AmpFlSTR® Identifiler® Kit for formaldehyde-fixed paraffin-embedded (FFPE) tissues in early gastric cancer patient with lymph node metastasis
After distal gastrectomy in a patient with
early gastric cancer, 27 regional lymph nodes around the
stomach were evaluated for the existence of metastasis.
There was a 0IIa+IIc type tumor 2.0x1.5cm in size in the
gastric angle of the lesser curvature according to the
Japanese Classification of Gastric Carcinoma (JCGC).
Histologically, the lesion extended no deeper than the
muscularis mucosae. The cancer stage was so early that
no metastasis was expected to occur but a lymph node
with metastasis was found in one lymph node along the
common anterior hepatic artery (station No.8a). This
histological type was a little different from that of a
primary tumor. The doctor began to suspect that the
lymph node with metastasis might have been from
another patient by mistake. Therefore, DNA typing
using the AmpFlSTR® Identifiler® kit was performed in
formaldehyde-fixed paraffin-embedded (FFPE) tissues:
2 parts of gastric mucosa without cancer, one part of
gastric mucosa with cancer, 4 lymph nodes without
metastasis, and the lymph node station No.8a with
metastasis. STR typing was successful in 6~14 STR loci
and amelogenin gene, and the detected STR type was the
same in all samples. Compared with the STR type using
DNA from the patient’s blood, the lymph node station
No.8a was from the same patient. The lymph node with
metastasis turned out to be not from another patient.
Therefore, we suggest that DNA typing using the
AmpFlSTR® Identifiler® Kit for FFPE samples is useful
in such clinical cases