Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

Whole-organ/body three-dimensional (3D) staining and imaging have been enduring challenges in histology. By dissecting the complex physicochemical environment of the staining system, we developed a highly optimized 3D staining imaging pipeline based on CUBIC. Based on our precise characterization of biological tissues as an electrolyte gel, we experimentally evaluated broad 3D staining conditions by using an artificial tissue-mimicking material. The combination of optimized conditions allows a bottom-up design of a superior 3D staining protocol that can uniformly label whole adult mouse brains, an adult marmoset brain hemisphere, an ~1 cm3 tissue block of a postmortem adult human cerebellum, and an entire infant marmoset body with dozens of antibodies and cell-impermeant nuclear stains. The whole-organ 3D images collected by light-sheet microscopy are used for computational analyses and whole-organ comparison analysis between species. This pipeline, named CUBIC-HistoVIsion, thus offers advanced opportunities for organ- and organism-scale histological analysis of multicellular systems

ポストカラム ケイコウ ユウドウカ ハンノウ オ ドウニュウシタ コウソク エキタイ クロマトグラフィー ニ ヨル カンゲントウ ビリョウ ブンセキホウ ノ カイハツ ト オウヨウ

京都大学0048新制・課程博士博士(農学)甲第14663号農博第1745号新制||農||968(附属図書館)学位論文||H21||N4436(農学部図書室)UT51-2009-D375京都大学大学院農学研究科食品生物科学専攻(主査)教授 井上 國世, 教授 安達 修二, 教授 入江 一浩学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDA