Dosimetric Comparisons of Volumetric Modulated Arc Therapy with Three-Dimensional Conformal Radiation Therapy for Locally Advanced Non-Small Cell Lung Cancer

Article信州医学雑誌 65(2): 93-98(2017)journal articl

Predictive Factors for Deterioration of Lower Urinary Tract Symptoms After Iodine-125 Brachytherapy in Prostate Cancer Patients

Article信州医学雑誌 66(2): 131-137(2018)journal articl

A Case of Sinonasal Undifferentiated Carcinoma Sucessfully Responding to Chemoradiotherapy

Article信州医学雑誌 69(3) : 121-126(2021)departmental bulletin pape

Validity of reduced radiation dose for localized diffuse large B-cell lymphoma showing a good response to chemotherapy

To evaluate the validity of a decrease in the radiation dose for patients who were good responders to chemotherapy for localized diffuse large B-cell lymphoma (DLBCL), 91 patients with localized DLBCL who underwent radiotherapy after multi-agent chemotherapy from 1988-2008 were reviewed. Exclusion criteria were as follows: central nervous system or nasal cavity primary site, or Stage II with bulky tumor (>= 10 cm). Of these patients, 62 were identified as good responders to chemotherapy. They were divided into two groups receiving either a higher or a lower radiation dose (32-50.4 Gy or 15-30.6 Gy, respectively). There were no statistically significant differences between the lower and higher dose groups in progression-free survival, locoregional progression-free survival or overall survival. Adaptation of decreased radiation dose may be valid for localized DLBCL patients who show a good response to chemotherapy.ArticleJOURNAL OF RADIATION RESEARCH. 55(2):359-363 (2014)journal articl

ジシ イゾク ノ グリーフワーク オ ソクシン スル ミンカン シンコウ ノ ジッタイ

自死遺族のグリーフワークにおいて、青森県の特徴的な民間信仰である「イタコ」の、どのような支えが喪失による悲嘆を乗り越えることができたかを明らかにすることを目的に、イタコを利用したことのある自死遺族で1年以上経過した30歳代～70歳代の女性７名に聞き取り調査を行った。遺族は、イタコの口寄せによって自殺の理由を聞かされたことで、故人の人生に対する価値を見出すことができ癒された。また遺族は、【故人の生き様への共感】、さらに、【故人の加護や繋がりの実感】【相互の赦免の獲得】を経て、【生きることへの託宣】を得て、【心の浄化】ができた。The early detection of relapse in patients with schizophrenia is considered an important objective for psychiatric nurses doing home health visits. In this study, interview surveys of home health psychiatric nurses were conducted to elucidate what they watched for when monitoring these patients for signs of relapse. The results showed that when monitoring for deteriorating health, through experience, they had learned to watch for deviations from usual lifestyle patterns, worsening of patient pathological experience, loss of interest in treatment, and abnormal communication

Screening of Thermotolerant Gluconobacter Strains for Production of 5-Keto-d-Gluconic Acid and Disruption of Flavin Adenine Dinucleotide-Containing d-Gluconate Dehydrogenase▿

We isolated thermotolerant Gluconobacter strains that are able to produce 5-keto-d-gluconic acid (5KGA) at 37°C, a temperature at which regular mesophilic 5KGA-producing strains showed much less growth and 5KGA production. The thermotolerant strains produced 2KGA as the major product at both 30 and 37°C. The amount of ketogluconates produced at 37°C was slightly less than the amount produced at 30°C. To improve the yield of 5KGA in these strains, we disrupted flavin adenine dinucleotide-gluconate dehydrogenase (FAD-GADH), which is responsible for 2KGA production. Genes for FAD-GADH were cloned by using inverse PCR and an in vitro cloning strategy. The sequences obtained for three thermotolerant strains were identical and showed high levels of identity to the FAD-GADH sequence reported for the genome of Gluconobacter oxydans 621 H. A kanamycin resistance gene cassette was used to disrupt the FAD-GADH genes in the thermotolerant strains. The mutant strains produced 5KGA exclusively, and the final yields were over 90% at 30°C and 50% at 37°C. We found that the activity of pyrroloquinoline quinone (PQQ)-dependent glycerol dehydrogenase, which is responsible for 5KGA production, increased in response to addition of PQQ and CaCl2 in vitro when cells were grown at 37°C. Addition of 5 mM CaCl2 to the culture medium of the mutant strains increased 5KGA production to the point where over 90% of the initial substrate was converted. The thermotolerant Gluconobacter strains that we isolated in this study provide a promising new option for industrial 5KGA production

l-Sorbose Reductase and Its Transcriptional Regulator Involved in l-Sorbose Utilization of Gluconobacter frateurii▿

Upstream of the gene for flavin adenine dinucleotide (FAD)-dependent d-sorbitol dehydrogenase (SLDH), sldSLC, a putative transcriptional regulator was found in Gluconobacter frateurii THD32 (NBRC 101656). In this study, the whole sboR gene and the adjacent gene, sboA, were cloned and analyzed. sboR mutation did not affect FAD-SLDH activity in the membrane fractions. The SboA enzyme expressed and purified from an Escherichia coli transformant showed NADPH-dependent l-sorbose reductase (NADPH-SR) activity, and the enzyme was different from the NADPH-SR previously reported for Gluconobacter suboxydans IFO 3291 in molecular size and amino acid sequence. A mutant defective in sboA showed significantly reduced growth on l-sorbose, indicating that the SboA enzyme is required for efficient growth on l-sorbose. The sboR mutant grew on l-sorbose even better than the wild-type strain did, and higher NADPH-SR activity was detected in cytoplasm fractions. Reverse transcription-PCR experiments indicated that sboRA comprises an operon. These data suggest that sboR is involved in the repression of sboA, but not in the induction of sldSLC, on d-sorbitol and that another activator is required for the induction of these genes by d-sorbitol or l-sorbose