ブランチポイントは遺伝性疾患に対するエクソンスキッピング療法の有望な標的である

京都大学新制・課程博士博士(医学)甲第24996号医博第5030号新制||医||1069(附属図書館)京都大学大学院医学研究科医学専攻(主査)教授 齊藤 博英, 教授 滝田 順子, 教授 小川 誠司学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

Pulsation-assisted fluidized bed for the fluidization of high moisture and irregular particles and its application for brown coal fluidization

High moisture content and irregular particle is difficult to be fluidized. This is due to the existence of large particles which would affect the fluidization performance. Also, a high moisture content would induce channeling during the fluidization. Pulsation-assisted fluidized bed (PAFB) with the combination of pulsed and steady flow as the fluidized gas was found to be able for the wet biomass particles fluidization [1]. In this study, fluidization performance of high moisture content particle with wide size distribution was investigated in both PAFB and steady fluidized bed. As shown in Fig. 1, oscillation was more serious and large bubbles were found in PAFB. The bubble size could be controlled in PAFB by adjusting the pulsed flow frequency. Results showed that compared with a steady-flow fluidized bed, pulsation-assisted flow (1~6 Hz) could increase the mass ratio of large particles at the bottom of the fluidized bed by over 10wt% (Fig. 2). Pulsation-assisted flow could also increase the mixing rate and break the liquid bridge between the solid particles by the large generated bubble. This could cause fluidization period 8 times than that with a steady flow, when high moisture content particles were added into the bed semi-continuously. Fluidization finally stopped due to the increasing bed moisture content (Fig. 3). A predicted model was developed to investigate the bubble size effect on the fluidization performance and compared with the experimental results. The optimal operation condition for the pulsed flow in PAFB was investigated based on the developed model. Please click Additional Files below to see the full abstract

Studies on Metal Organic Framework Nanofilms Assembled on Polymer Surfaces

要約のみTohoku University三ツ石方也課

Development of a Novel Reformer for Tar-free Syngas Production

AbstractA novel reformer using highly efficient heat regeneration for tar-free syngas production is developed and its performance demonstrated in a pilot-scale plant using steam gasification. Basic design parameters of the regenerative tar reformer, namely residence time and amount of oxidant are determined based on numerical results. It has been predicted that good performance could be achieved at an operation temperature about 1573K, the residence time exceeding 4sec and an oxidant addition of 12% of the syngas flow rate. The regenerative tar reformer so designed shows stable operation. Over 99% of light and heavy tars are reformed to gas in the case of 11.3% oxygen addition to syngas. Further it is seen that a reduction of oxygen consumption more than 30% compared to a conventional oxidation reformer can be achieved. The formation of a high temperature zone has a strong influence on the tar reforming efficiency

Oligo-recurrence from anaplastic lymphoma kinase-rearranged lung adenocarcinoma

Anaplastic lymphoma kinase rearranged non-small-cell lung cancer is a rare disease. Among them, a subset of patients exist who exhibit relatively slowly progressing symptoms and have oligo-metastases. In this article, we present two cases of ALK rearran-ged lung adenocarcinoma in patients who experienced postoperative oligo-recurrence. Both cases were treated with surgical resection and gamma knife irradiation for oligo-recurrence. After local therapy, the first patient remained disease free for over  23 months; the second for over 18 months. It appears that some patients with ALK rearranged NSCLC experience oligo-recurrence in their clinical course. For such patients, appropriate local therapy may be beneficial in improving both the quality of life and the prognosis

Nintedanib-mediated improvement in CT imaging in pulmonary fibrosis associated with systemic scleroderma

Nintedanib is an antifibrotic drug that has an inhibitory effect on growth factor tyrosine kinases. In patients with idiopathic pulmonary fibrosis and systemic scleroderma-associated interstitial pneumonia (SSc-IP), nintedanib has been effective in suppressing the decline in forced vital capacity over time and the onset of acute exacerbation of interstitial pneumonia. Here, we report a SSc-IP patient who showed an improvement on CT images following nintedanib treatment. To our knowledge, this is the first report of such a case. Although SSc-IP patients are very rare, additional clinical experience and understanding will be required to prove the therapeutic benefit of nintedanib in these cases in relation to improved chest images

Roles of Porphyromonas gulae proteases in bacterial and host cell biology

Porphyromonas gulae, an animal-derived periodontal pathogen, expresses several virulence factors, including fimbria, lipopolysaccharide (LPS) and proteases. We previously reported that its invasive efficiency was dependent on fimbriae types. In addition, P. gulae LPS increased inflammatory responses via toll-like receptors. The present study was conducted to investigate the involvement of P. gulae proteases in bacterial and host cell biology. Porphyromonas gulae strains showed an ability to agglutinate mouse erythrocytes and also demonstrated co-aggregation with Actinomyces viscosus, while the protease inhibitors antipain, PMSF, TLCK and leupeptin diminished P. gulae proteolytic activity, resulting in inhibition of haemagglutination and co-aggregation with A. viscosus. In addition, specific proteinase inhibitors were found to reduce bacterial cell growth. Porphyromonas gulae inhibited Ca9-22 cell proliferation in a multiplicity of infection- and time-dependent manner. Additionally, P. gulae-induced decreases in cell contact and adhesion-related proteins were accompanied by a marked change in cell morphology from well spread to rounded. In contrast, inhibition of protease activity prevented degradation of proteins, such as E-cadherin, beta-catenin and focal adhesion kinase, and also blocked inhibition of cell proliferation. Together, these results indicate suppression of the amount of human proteins, such as gamma-globulin, fibrinogen and fibronectin, by P. gulae proteases, suggesting that a novel protease complex contributes to bacterial virulence