3 research outputs found
Permselectivity of the glomerular wall examined with iron compound tracer
Rat kidney endothelial cell morphology was examined
after introducing iron colloid particles of positive or negative charge
to investigate the relationship between the electric charge and permeation
through the glomerular capillary. The kidneys were first
perfused with Hanks' solution through the renal arteries and then
with iron colloid particles of positive or negative charge. The iron
colloid particles of positive charge were prepared with ferric chloride
and cacodylate solutions, and the negative particles were prepared
with iron chondroitin sulfate colloid particles. The iron colloid particles
of positive charge adhered to the surface of endothelial cells of
the glomerular capillaries, as well as the arterioles, capillaries and
venules. Some particles were taken up by pinocytosis, accumulated
in the glomerular basement membrane and appeared in the urinary
spaces passing through the filtration slits of podocytes. Iron colloid
particles of negative charge neither adhered to the endothelial cells
nor were taken by the cells. They did not permeate into the urinary
spaces. Permeation into the tubular lumen through the peritubular
venules was not observed with particles of positive or negative charge.</p