1,988 research outputs found
Neutron and X-ray Diffraction Study on the Location of Hydrogen Isotopes in TiO0.3 and ZrO0.4
Measuring the brightness temperature distribution of extragalactic radio sources with space VLBI
We have used VSOP space very long baseline interferometry observations to
measure the brightness temperature distribution of a well-defined sub-set of
the Pearson-Readhead sample of extragalactic radio sources. VLBI which is
restricted to Earth-diameter baselines is not generally sensitive to emitting
regions with brightness temperatures greater than approximately K,
coincidentally close to theoretical estimates of brightness temperature limits,
K. We find that a significant proportion of our sample have
brightness temperatures greater than K; many have unresolved
components on the longest baselines, and some remain completely unresolved.
These observations begin to bridge the gap between the extended jets seen with
ground-based VLBI and the microarcsecond structures inferred from intraday
variability, evidenced here by the discovery of a relationship between intraday
variability and VSOP-measured brightness temperature, likely due to the effects
of relativistic beaming. Also, lower limits on jet Lorentz factors, estimated
from space VLBI observations, are starting to challenge numerical simulations
that predict low Lorentz factor jets.Comment: 4 pages + 1 figure, ApJ letters, accepte
Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries
We have recently reported that multiple aster formation after IVF was one of the factors negatively affecting to developmental competence of vitrified-warmed bovine matured oocytes, and that a short-term culture of the post-warm oocytes with an inhibitor of Rho-associated coiled-coil kinase (ROCK) suppressed the multiple aster formation and improved the blastocyst yield. The present study was conducted to investigate whether increased multiple aster formation following IVF can be involved in impaired developmental competence of stored ovary-derived bovine oocytes. Oocytes retrieved from 1-day stored ovaries had lower developmental potential to Day 8 blastocysts when compared with those from fresh ovaries (37 vs 63%). Immunostaining against -tubulin 10 h post-IVF revealed that the higher incidence of multiple aster formation occurred in oocytes retrieved from stored ovaries than fresh ovaries (31 vs 15%). Treatment of post-IVM oocytes with ROCK inhibitor for 2 h significantly suppressed the incidence of multiple aster formation (10 vs 32% in control group). However, the suppressing effect of ROCK inhibitor on multiple aster formation in IVM/IVF oocytes did not improve blastocyst yield from stored ovary-derived oocytes (41 vs 37% in control group). These results suggest that higher incidence of multiple aster formation by bovine ovary storage was not responsible for decreased developmental competence of IVF oocytes.ArticleZYGOTE.24(1):115-120(2015)journal articl
A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa
Regardless of the presence of sperm-borne oocyte-activating factors, activation of bovine oocytes with exogenous activation stimuli is required for further development after intracytoplasmic sperm injection (ICSI). The current study was designed to develop a new activation regimen for improving the blastocyst yield after ICSI of bovine oocytes harvested from ovaries stored at 10 to 12 degrees C for 24 h. After ICSI, oocytes were treated with 5 mu M ionomycin for 5 min, 7% ethanol for 5 or 10 min, ionomycin followed by ethanol (5 or 10 min), ionomycin followed by 10 mu g/mL cycloheximide for 5 h, or ionomycin followed by 1.9 mM 6-dimethylaminopurine for 3 h. Across the activation regimens, the cleavage rates of ICSI oocytes (45% to 77%) were higher than those of parthenogenetically activated oocytes (11% to 21%; P 0.05). Higher blastocyst yields were due to increasing the proportion of ICSI oocytes that passed through the early postfertilization events until cleavage. None of the regimens have any adverse effect on the quality of the blastocysts regarding the total cell number or the proportion of the inner cell mass cells. Thus, a new activation regimen using two triggers for single calcium increase effectively improved blastocyst yield after bovine ICSI using oocytes harvested from stored ovaries.ArticleTHERIOGENOLOGY. 72(4):453-460 (2009)journal articl
Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year
ArticleMolecular Reproduction and Development. 75(5): 890-894 (2008)journal articl
Dual-Frequency VSOP Observations of AO 0235+164
AO 0235+164 is a very compact, flat spectrum radio source identified as a BL
Lac object at a redshift of z=0.94. It is one of the most violently variable
extragalactic objects at both optical and radio wavelengths. The radio
structure of the source revealed by various ground-based VLBI observations is
dominated by a nearly unresolved compact component at almost all available
frequencies.
Dual-frequency space VLBI observations of AO 0235+164 were made with the VSOP
mission in January-February 1999. The array of the Japanese HALCA satellite and
co-observing ground radio telescopes in Australia, Japan, China and South
Africa allowed us to study AO 0235+164 with an unprecedented angular resolution
at frequencies of 1.6 and 5 GHz. We report on the sub-milliarcsecond structural
properties of the source. The 5-GHz observations led to an estimate of T_B >
5.8 x 10^{13} K for the rest-frame brightness temperature of the core, which is
the highest value measured with VSOP to date.Comment: 8 pages, 8 figures, to appear in Publ. Astron. Soc. Japa
Estimation of Direct and Indirect Economic Losses Caused by a Flood With LongâLasting Inundation: Application to the 2011 Thailand Flood
River floods are common natural disasters that cause serious economic damage worldwide. In addition to direct economic damage, such as the destruction of physical assets, floods with longâlasting inundation cause direct and indirect economic losses within and outside the affected area. Direct economic losses include loss of opportunity, due to interruption of business activities, and the costs associated with emergency measures such as cleaning, while indirect economic losses affect sectors within the trade and supply network. Few studies have explicitly estimated direct and indirect economic losses in several sectors, due to the difficulty of modeling inundation depth and period at finer scales. Here we developed a global modeling framework to estimate the direct and indirect economic losses associated with floods using a computable general equilibrium model and a global river and inundation model, which can simulate the flood extent, depth, and period. Application of the method to the 2011 Thailand flood demonstrated that the estimated economic losses due to business interruption in the industry and service sectors totaled 22.0 billion). The estimated indirect economic losses reduced the gross domestic product of Thailand by 4.81% in 2011, without considering transboundary effects, and will cause more than 0.5% reduction in gross domestic product even in 2030, resulting in $55.3 billion of total losses from 2011 to 2030. Comprehensive estimation of direct and indirect economic losses facilitates understanding of various types of floodârelated economic risks during and after a flood
High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization
In vitro-matured bovine oocytes do not tolerate vitrification as well as mature murine or human oocytes. Delayed first cleavage in vitrified and in vitro-fertilized bovine oocytes may be responsible for the decreased yield of blastocysts in vitro. Because formation of sperm-aster and the subsequent assembly of microtubule network play an important role for migration and fusion of both pronuclei, aster formation in vitrified-warmed oocytes was analyzed by confocal laser-scanning microscopy. At 10 h post-insemination (hpi), proportions of oocytes fertilized normally were comparable between the vitrified and fresh control groups (67 and 70%, respectively). Proportions of oocytes that exhibited microtubule assembly were similar between the two groups (95% each), but the proportion of oocytes with multiple asters was higher in the vitrified group when compared with the fresh control group (68 vs 29%, P < 0.05). Both migration and development of two pronuclei were adversely affected by multiple aster formation. In the next experiment, multiple asters observed in 5.5 vs 8 hpi pronuclear zygotes were located near the male pronucleus, suggesting that those multiple asters were not the cytoplasmic asters of maternal origin. In conclusion, multiple aster formation frequently observed in vitrified-warmed bovine oocytes may be related to loss of ooplasmic function responsible for normal microtubule assembly from the sperm-aster.ArticleTHERIOGENOLOGY. 77(5):908-915 (2012)journal articl
Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification
Inhibition of Rho-associated kinase (ROCK) activity promoted recovery and growth of frozen-thawed human embryonic stem cells. The primary objective was to determine if a ROCK inhibitor (Y-27632) in post-thaw culture medium unproved revivability of vitrified IVP bovine blastocysts. Expanding or expanded blastocysts (7 d after IVF) were vitrified (minimum volume cooling procedure, using a Cryotop) in 15% ethylene glycol. 15% DMSO and 0 5 M sucrose When post-warm blastocysts were cultured in inSOF medium, survival rate (re-expansion of blastocoel at 24 h of culture) was improved (P < 0 05) by the addition of 10 mu M Y-27632 (94 9 +/- 2 4%, mean +/- SEM) compared to a control (78 0 +/- 6 0%) Conversely. after 48 h of culture, there were no significant differences in hatching late (62.8 +/- 11 1 vs 59 6 +/- 9.4%) and mean total cell number (135 2 +/- 13 1 vs. 146 7 +/- 13 3) In non-vitrified IVP bovine blastocysts, the hatching rate on Day 9 was improved by Y-27632 (91 7 +/- 3 8 vs 54 7 +/- 8 9%. P < 0 05). with no difference in mean total cell number of blastocysts (230 0 +/- 23 0 vs 191 2 +/- 22 2, P = 0 23). In an additional experiment, Y-27632 was added to culture medium on either Day 0, Day 2. or Day 4 (and remained present until Day 8). resulting in no improvement in blastocyst yield compared to a control group (7.5 +/- 2 1, 31 4 +/- 2 3, 36 2 +/- 3.2. and 28.6 +/- 6.9%. respectively) In conclusion, adding a ROCK inhibitor to post-thaw culture medium improved revivability of IVP bovine blastocysts after vitrification and warmingArticleTHERIOGENOLOGY. 73(8):1139-1145 (2010)journal articl
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