1,091 research outputs found
Concanavalin A-Binding Enzymes of Crotalus scutulatus scutulatus Venom
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The proteins binding to Con A exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC 3.1.3.2), phosphodiesterase, 5\u27-nucleotidase (5\u27-ribonucleotide phosphohydrolase EC 3.1.3.5), phospholipase A(phosphatidate 2-acylhydrolase EC 3.1.1 .4), hyaluronidase (hyaluronate glycanohydrolase EC 3.2.1 d), N-benzoyl-L-arginine ethyl esterase, p-toluenesulfonyl-L-arginine methyl esterase, L-amino acid oxidase (L-amino acid: 02 oxidoreductase [deaminating] EC 1.4.3.2), and caseinolytic activities. Thrombin-like and NAD nucleosidase (5\u27-ribonucleotide phosphohydrolase EC 3.1.3.5) activities were not observed. The crude venom and the fraction containing the glycoproteins which bound to Con A were fractionated by DEAE Sephadex A-50 ion exchange chromatography. Each of these samples yielded fractions having caseinolytic activities
Concanavalin A-Nonbinding Enzymes of Crotalus scutulatus scutulatus Venom
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The Concanavalin A-nonbinding fraction (F-l) exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC 3.1 .3.2), phosphodiesterase, 5 \u27-nucleotidase (5 \u27-ribonucleotide phosphohydrolase EC 3.1.3.5), phospholipase A (phosphatidate 2-acylhydrolase EC 3.1.1.4), hyaluronidase (hyaluronate glycanohydrolase EC 3.2.1.d), N-benzoyl-Larginine ethyl esterase, p-toluenesulfonyl-L-arginine methyl esterase, L-amino acid oxidase (L-amino acid: O2 oxidoreductase [deaminating] EC 1.4.3.2), and caseinolytic activities. Thrombin-like and NAD nucleosidase (5 \u27-ribonudeotide phosphohydrolase EC 3.1.3.5) activities were not observed. DEAE Sephadex A-50 ion exchange chromatography by two stage elution of F-l yielded several fractions having proteinase activities. Proteinase activity was observed in the latter fractions of the first elution and in the fractions of the second elution
Update to the Vitamin C, Thiamine and Steroids in Sepsis (VICTAS) protocol: statistical analysis plan for a prospective, multicenter, double-blind, adaptive sample size, randomized, placebo-controlled, clinical trial.
BACKGROUND: Observational research suggests that combined therapy with Vitamin C, thiamine and hydrocortisone may reduce mortality in patients with septic shock.
METHODS AND DESIGN: The Vitamin C, Thiamine and Steroids in Sepsis (VICTAS) trial is a multicenter, double-blind, adaptive sample size, randomized, placebo-controlled trial designed to test the efficacy of combination therapy with vitamin C (1.5 g), thiamine (100 mg), and hydrocortisone (50 mg) given every 6 h for up to 16 doses in patients with respiratory or circulatory dysfunction (or both) resulting from sepsis. The primary outcome is ventilator- and vasopressor-free days with mortality as the key secondary outcome. Recruitment began in August 2018 and is ongoing; 501 participants have been enrolled to date, with a planned maximum sample size of 2000. The Data and Safety Monitoring Board reviewed interim results at N = 200, 300, 400 and 500, and has recommended continuing recruitment. The next interim analysis will occur when N = 1000. This update presents the statistical analysis plan. Specifically, we provide definitions for key treatment and outcome variables, and for intent-to-treat, per-protocol, and safety analysis datasets. We describe the planned descriptive analyses, the main analysis of the primary end point, our approach to secondary and exploratory analyses, and handling of missing data. Our goal is to provide enough detail that our approach could be replicated by an independent study group, thereby enhancing the transparency of the study.
TRIAL REGISTRATION: ClinicalTrials.gov, NCT03509350. Registered on 26 April 2018
Observation of the Isospin-Violating Decay
Using data collected with the CLEO~II detector, we have observed the
isospin-violating decay . The decay rate for this mode,
relative to the dominant radiative decay, is found to be .Comment: 8 page uuencoded postscript file, also available through
http://w4.lns.cornell.edu/public/CLN
Search for CP Violation in D^0--> K_S^0 pi^+pi^-
We report on a search for CP violation in the decay of D0 and D0B to Kshort
pi+pi-. The data come from an integrated luminosity of 9.0 1/fb of e+e-
collisions at sqrt(s) ~ 10 GeV recorded with the CLEO II.V detector. The
resonance substructure of this decay is well described by ten quasi-two-body
decay channels (K*-pi+, K*0(1430)-pi+, K*2(1430)-pi+, K*(1680)-pi+, Kshort rho,
Kshort omega, Kshort f0(980), Kshort f2(1270), Kshort f0(1370), and the ``wrong
sign'' K*+ pi-) plus a small non-resonant component. We observe no evidence for
CP violation in the amplitudes and phases that describe the decay D0 to K_S^0
pi+pi-.Comment: 10 pages, 3 figures, also available at
http://w4.lns.cornell.edu/public/CLNS/, submitted to PR
Measurement of the Partila Width and Form Factor Parameters
We have studied the decay , where . From a fit to the differential decay rate we measure the
rate normalization and form factor slope
, and, using measured values of , find . The resulting branching
fractions are and .
The form factor parameters are in agreement with those measured in decays, as predicted by heavy quark effective theory.Comment: 11 pages, postscript file also available through
http://w4.lns.cornell.edu/public/CLN
Observation of a Narrow Resonance of Mass 2.46 GeV/c^2 Decaying to D_s^*+ pi^0 and Confirmation of the D_sJ^* (2317) State
Using 13.5 inverse fb of e+e- annihilation data collected with the CLEO II
detector we have observed a narrow resonance in the Ds*+pi0 final state, with a
mass near 2.46 GeV. The search for such a state was motivated by the recent
discovery by the BaBar Collaboration of a narrow state at 2.32 GeV, the
DsJ*(2317)+ that decays to Ds+pi0. Reconstructing the Ds+pi0 and Ds*+pi0 final
states in CLEO data, we observe peaks in both of the corresponding
reconstructed mass difference distributions, dM(Dspi0)=M(Dspi0)-M(Ds) and
dM(Ds*pi0)=M(Ds*pi0)-M(Ds*), both of them at values near 350 MeV. We interpret
these peaks as signatures of two distinct states, the DsJ*(2317)+ plus a new
state, designated as the DsJ(2463)+. Because of the similar dM values, each of
these states represents a source of background for the other if photons are
lost, ignored or added. A quantitative accounting of these reflections confirms
that both states exist. We have measured the mean mass differences
= 350.0 +/- 1.2 [stat] +/- 1.0 [syst] MeV for the DsJ*(2317) state, and
= 351.2 +/- 1.7 [stat] +/- 1.0 [syst] MeV for the new DsJ(2463)+
state. We have also searched, but find no evidence, for decays of the two
states via the channels Ds*+gamma, Ds+gamma, and Ds+pi+pi-. The observations of
the two states at 2.32 and 2.46 GeV, in the Ds+pi0 and Ds*+pi0 decay channels
respectively, are consistent with their interpretations as (c anti-strange)
mesons with orbital angular momentum L=1, and spin-parities of 0+ and 1+.Comment: 16 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLNS, version to be published in Physical
Review D; minor modifications and fixes to typographical errors, plus an
added section on production properties. The main results are unchanged; they
supersede those reported in hep-ex/030501
Branching Fractions of tau Leptons to Three Charged Hadrons
From electron-positron collision data collected with the CLEO detector
operating at CESR near \sqrt{s}=10.6 GeV, improved measurements of the
branching fractions for tau decays into three explicitly identified hadrons and
a neutrino are presented as {\cal
B}(\tau^-\to\pi^-\pi^+\pi^-\nu_\tau)=(9.13\pm0.05\pm0.46)%, {\cal B}(\tau^-\to
K^-\pi^+\pi^-\nu_\tau)=(3.84\pm0.14\pm0.38)\times10^{-3}, {\cal B}(\tau^-\to
K^-K^+\pi^-\nu_\tau)=(1.55\pm0.06\pm0.09)\times10^{-3}, and {\cal B}(\tau^-\to
K^-K^+K^-\nu_\tau)<3.7\times10^{-5} at 90% C.L., where the uncertainties are
statistical and systematic, respectively.Comment: 10 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLNS, to appear in Phys. Rev. Let
Improved Measurement of the Form Factors in the Decay Lambda_c^+ --> Lambda e^+ nu_e
Using the CLEO detector at the Cornell Electron Storage Ring, we have studied
the distribution of kinematic variables in the decay Lambda_c^+ -> Lambda e^+
nu_e. By performing a four-dimensional maximum likelihood fit, we determine the
form factor ratio, R = f_2/f_1 = -0.31 +/- 0.05(stat) +/- 0.04(syst), the pole
mass, M_{pole} = (2.21 +/- 0.08(stat) +/- 0.14(syst)) GeV/c^2, and the decay
asymmetry parameter of the Lambda_c, alpha_{Lambda_c} = -0.86 +/- 0.03(stat)
+/- 0.02(syst), for = 0.67 (GeV/c^2)^2. We compare the angular
distributions of the Lambda_c^+ and Lambda_c^- and find no evidence for
CP-violation: A_{Lambda_c} = (alpha_{Lambda_c^+} + alpha_{Lambda_c^-})/
(alpha_{Lambda_c^+} - alpha_{Lambda_c^-}) = 0.00 +/- 0.03(stat) +/- 0.01(syst)
+/- 0.02, where the third error is from the uncertainty in the world average of
the CP-violating parameter, A_{Lambda}, for Lambda -> p pi^-.Comment: 8 pages postscript,also available through
http://www.lns.cornell.edu/public/CLNS/2004/, submitted to PR
Anti-Search for the Glueball Candidate f_J(2220) in Two-Photon Interactions
Using 13.3 fb^{-1} of e^+e^- data recorded with the CLEO II and CLEO II.V
detector configurations at CESR, we have searched for f_J(2220) decays to
K^0_{S} K^0_{S} in untagged two-photon interactions. We report an upper limit
on the product of the two-photon partial width and the branching fraction,
Gamma_gamma gamma cdot B (f_J(2220) to K^0_{S} K^0_{S}) of less than 1.1 eV at
the 95% C.L: systematic uncertainties are included. This dataset is four times
larger than that used in the previous CLEO publication.Comment: 10 pages postscript, also available through
http://w4.lns.cornell.edu/public/CLNS, Submitted to PRD (R
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