EVALUATION OF GENETIC DIVERSITY OF THE BLACK GLUTINOUS RICE BASED ON AGRO-MORPHOLOGICAL CHARACTERS

The study assessed the variations in nine agro-morphological characters among and within the black glutinous rice (Oryza sativa) population from Chau Thanh District, Tra Vinh Province. The nine quantitative agromorphological characters that were measured include culm length, leaf length, leaf width, number of panicles, panicle length, grain length, grain width, number of firm grain, and number of grain per panicle. The unweighted pair group method with arithmetic mean method and principal coordinate analysis by the NTSYS program were applied in this study to classify the nine agro-morphological characters. In addition, to compare the variations in quantitative characters between O. sativa populations, one-way analysis of variance (ANOVA) was used. The results showed significant differences between the black glutinous rice populations for all quantitative agro-morphological characters. Moreover, some agro-morphological characters showed positive correlations to each other. The dendrogram generated from the analysis process of the agromorphological data divided the O. sativa populations into two groups with unfamiliar features. However, the O. sativa populations assessed exhibited a wide range of variations in morphological characteristics, both within the same population and among other populations with the same strains

CHEMICAL CHARACTERISTICS AND GELLING PROPERTIES OF AGAR EXTRACTED FROM RED ALGAE GROWING AT VIETNAM COAST

CHEMICAL CHARACTERISTICS AND GELLING PROPERTIES OF AGAR EXTRACTED FROM RED ALGAE GROWING AT VIETNAM COAS

Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i>

Ulvan, a sulfated heteropolysaccharide with structural and functional properties of interest for various uses, was extracted from the green seaweed Ulva papenfussii. U. papenfussii is an unexplored Ulva species found in the South China Sea along the central coast of Vietnam. Based on dry weight, the ulvan yield was ~15% (w/w) and the ulvan had a sulfate content of 13.4 wt%. The compositional constitution encompassed L-Rhamnose (Rhap), D-Xylose (Xylp), D-Glucuronic acid (GlcAp), L-Iduronic acid (IdoAp), D-Galactose (Galp), and D-Glucose (Glcp) with a molar ratio of 1:0.19:0.35:0.52:0.05:0.11, respectively. The structure of ulvan was determined using High-Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FT-IR), and Nuclear Magnetic Resonance spectroscopy (NMR) methods. The results showed that the extracted ulvan comprised a mixture of two different structural forms, namely (“A3s”) with the repeating disaccharide [→4)-β-D-GlcAp-(1→4)-α-L-Rhap 3S-(1→]n, and (“B3s”) with the repeating disaccharide [→4)-α-L-IdoAp-(1→4)-α-L-Rhap 3S(1→]n. The relative abundance of A3s, and B3s was 1:1.5, respectively. The potential anticarcinogenic attributes of ulvan were evaluated against a trilogy of human cancer cell lineages. Concomitantly, Quantitative Structure–Activity Relationship (QSAR) modeling was also conducted to predict potential adverse reactions stemming from pharmacological interactions. The ulvan showed significant antitumor growth activity against hepatocellular carcinoma (IC50 ≈ 90 µg/mL), human breast cancer cells (IC50 ≈ 85 µg/mL), and cervical cancer cells (IC50 ≈ 67 µg/mL). The QSAR models demonstrated acceptable predictive power, and seven toxicity indications confirmed the safety of ulvan, warranting its candidacy for further in vivo testing and applications as a biologically active pharmaceutical source for human disease treatment