Crystallization and preliminary X-ray structural studies of human prouroguanylin. Corrigendum

A corrigendum to the article by Ito et al. [Acta Cryst. (2008). F64, 531–532]

Mode of disulfide bond formation of a heat-stable enterotoxin (STh) produced by a human strain of enterotoxigenic Escherichia coli

AbstractTo determine the modes of three disulfide linkages in the heat-stable enterotoxin (STh) produced by a human strain of enterotoxigenic Escherichia coli, we synthesized STh(6–18), which consists of 13 amino acid residues and has the same intramolecular disulfide linkages as native STh [(1985) FEBS Lett. 181, 138–142], by stepwise and selective formation of disulfide bonds using different types of removable protecting groups for the Cys residues. Synthesis of the peptide with different modes of disulfide bond formation provided three peptides consistent with standard STh(6–18) in their physicochemical and biological properties, thereby indicating that the disulfide bonds in STh(6–18) are

SHRIMP U-Pb ages of the Karasaki mylonites in western Shikoku, Japan

唐崎マイロナイトの優白質角閃岩マイロナイトについて, ジルコンのSHRIMP U-Pb年代測定を行った結果, 1試料からの8粒子は114.2±3.9～102.3±4.5Ma(±1σ)の年代値を示した.マイロナイト化した優白質角閃岩は, SiO_2含有量が低い(49wt.%)ことと, 主として角閃石, 曹長石, 緑泥石および緑簾石のモード比の違いによる数百μm～数mm幅の顕著な層状構造を有することから, 玄武岩質火山岩または火山砕屑岩に由来する砂岩を原岩としている可能性が高い.優白質角閃岩マイロナイト中の丸みを帯びたジルコンは, ある程度遠方より運搬される過程で円磨された可能性が高いこと, マグマから晶出したジルコンに特徴的なオシレトリー累帯構造が認められることから, 砕屑性起源のジルコンであると判断される.また, その年代値が約110Maの狭い範囲を示す事実は, ジルコンが"古領家帯"に帰属すると考えられる真穴帯の大島変成岩や肥後帯の約110Ma深成岩類のみに由来する可能性を示唆する.したがって, 本論において初めて"古領家帯"中にアルビアン以降の堆積岩源変成岩が存在していることが明らかとなった.Zircon U-Pb isotope ages have been analyzed by the Sensitive High Resolution Ion MicroProbe (SHRIMP) in an leucocratic amphibolite mylonite from the Karasaki mylonites, western Shikoku, Japan which has been considered to belong to the "Paleo-Ryoke" belt. Eight zircon grains from the sample yielded U-Pb ages of Albian (Early Cretaceous) ranging from 114.2 ± 3.9 Ma to 102.3 ±4.5 Ma (± 1σ errors). The protolith of the mylonitized leucocratic amphibolite has been inferred to be basaltic volcanic sandstones, because the SiO_2 content is low (49 wt.%), and the leucocratic amphibolite (consisting of albite and epidote rich, and chlorite and epidote rich layers alternates with amphibole rich and chlorite rich layers on the order of a few mm, suggestive of original sedimentary layering. All the zircon grains can be inferred to be detrital based on both the facts that they were abraded possibly due to sedimentation processes, and that they show oscillatory zoning indicating igneous (primary) texture. The small range of U-Pb ages varying ca. 110 Ma suggests that the zircon grains in the volcanic sandstones were supplied only from the Oshima metamorphic rocks of the Maana belt and the Higo plutonic rocks of the Higo belt whose U-Pb zircon ages were dated as ca. 110 Ma. The fact clearly indicates that the metamorphic rocks (Karasaki mylonites) originated from post-Albian sediments also constitute as a new member of the "Paleo-Ryoke" belt

Crossovers within a short DNA sequence indicate a long evolutionary history of the APRT*J mutation

Adenine phosphoribosyltransferase (APRT) deficiency causing 2,8-dihydroxyadenine urolithiasis and renal failure is present at a high frequency among the Japanese but not other ethnic groups. A special type of mutant allele, designated APRT*J , with a nucleotide substitution at codon 136 from ATG (Met) to ACG (Thr) is carried by approximately 79% of all Japanese 2,8-dihydroxyadenine urolithiasis patients. We analyzed mutant alleles of 39 APRT deficient patients using a specific oligonucleotide hybridization method after in vitro amplification of a part of the genomic APRT sequence. We found that 24 had only APRT*J alleles. Determination of the haplotypes of 194 APRT alleles from control Japanese subjects and of the 48 different APRT*J alleles indicated that normal alleles occur in four major haplotypes, whereas all APRT*J alleles occur in only two. These results suggest that all APRT*J alleles have a single origin and that this mutant sequence has been maintained for a long period, as calculated from the frequency of the recombinant alleles.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47628/1/439_2004_Article_BF00193582.pd

Relationship between sintering temperature and bending strength for Y2O3 partially stabilized zirconia

The objective of this study was to verify the stability of Y2O3 partially stabilized zirconia (zirconia) sintered at 1,350℃ and 1,450℃ in a simulated oral environment over a long period of time. After sintering, zirconia was immersed in physiological saline, 1% lactic acid solution, and 1% malic acid solution which can be produced in the oral cavity for 3 or 6 months. The bending strength and strain were measured. The following results were obtained. Before immersion, there was no difference in the bending strength of zirconia sintered at 1,350℃ and 1,450℃. For zirconia sintered at 1,350℃, there was no difference among solutions in the bending strength after exposure. However, for zirconia sintered at 1,450℃, the bending strength and strain were lower after exposure to 1% lactic acid solution for 3 months. There was no effect on the strain after exposure to any solutions for zirconia sintered at 1,350℃. For zirconia sintered at 1,350℃, fracture was not observed during exposure to any of the solutions. However, when sintered at 1,450℃, zirconia was fractured during exposure to 1% lactic acid solution and 1% malic acid solution for 6 months. It was clarified that zirconia sintered at 1,350℃ was more suitable than that sintered at 1,450℃ as an implant material

The quarkynic phase and the Z_{Nc} symmetry

We investigate the interplay between the Z_{Nc} symmetry and the emergence of the quarkyonic phase, adding the flavor-dependent complex chemical potentials \mu_f=\mu+iT\theta_f with (\theta_f)=(0, \theta, -\theta) to the Polyakov-loop extended Nambu-Jona-Lasinio (PNJL) model. When \theta=0, the PNJL model with the \mu_f agrees with the standard PNJL model with the real chemical potential \mu. When \theta=2\pi/3, meanwhile, the PNJL model with the \mu_f has the Z_{Nc} symmetry exactly for any real \mu, so that the quarkyonic phase exists at small T and large \mu. Once \theta varies from 2\pi/3, the quarkyonic phase exists only on a line of T=0 and \mu larger than the dynamical quark mass, and the region at small T and large \mu is dominated by the quarkyonic-like phase in which the Polyakov loop is small but finite.Comment: 5 pages, 6 figure