Requirement of a Membrane Potential for the Posttranslational Transfer of Proteins into Mitochondsria

Posttranslational transfer of most precursor proteins into mitochondria is dependent on energization of the mitochondria. Experiments were carried out to determine whether the membrane potential or the intramitochondrial ATP is the immediate energy source. Transfer in vitro of precursors to the ADP/ATP carrier and to ATPase subunit 9 into isolated Neurospora mitochondria was investigated. Under conditions where the level of intramitochondrial ATP was high and the membrane potential was dissipated, import and processing of these precursor proteins did not take place. On the other hand, precursors were taken up and processed when the intramitochondrial ATP level was low, but the membrane potential was not dissipated. We conclude that a membrane potential is involved in the import of those mitochondrial precursor proteins which require energy for intracellular translocatio

N(2) Fixation by Azospirillum brasilense and Its Incorporation into Host Setaria italica

Growth and nitrogen fixation were followed during the life cycle of Setaria italica (foxtail millet) inoculated with Azospirillum brasilense in controlled-environment growth chambers. The plants were fertilized at seeding with a limiting amount of combined nitrogen and maintained with an N-free mineral solution. During maturation of the plants, substantial nitrogenase activity, measured by acetylene reduction, developed in the rhizosphere, with total fixation estimated to be equivalent to 20% of the N in the inoculated plants. The peak of this activity coincided with depletion of soluble nitrogen from the system, which in turn was reflected by a sharp decrease in the nitrate reductase activity of the leaves. A. brasilense was found in association with the root populations at 8 × 10(7) cells per gram of dry weight. An increase in shoot growth occurred at this time, but no significant increase in total plant nitrogen could be demonstrated. (15)N(2) enrichment experiments confirmed that fixation was occurring, but only about 5% of the nitrogen fixed by A. brasilense was incorporated into the plants within 3 weeks. There was thus no evidence of direct bacterium-to-plant transport of fixed nitrogen, but rather a slow transfer suggesting the gradual death of bacteria and subsequent mineralization of their nitrogen, at least under growth-room conditions

Trends and perspectives in flexible and reconfigurable manufacturing systems

To better understand future needs in manufacturing and their enabling technologies, a survey of experts in manufacturing has been conducted. The survey instrument (i.e., questionnaire) tries to assess the experience to date with the use of flexible manufacturing systems (FMS) and to examine the potential roles and enabling technologies for reconfigurable manufacturing systems (RMS). The results show that two-thirds of respondents stated that FMSs are not living up to their full potential, and well over half reported purchasing FMS with excess capacity (which was eventually used) and excess features (which in many cases were not eventually used). They identified a variety of problems associated with FMS, including training, reconfigurability, reliability and maintenance, software and communications, and initial cost. However, despite these issues, nearly 75% of respondent expressed their desire to purchase additional, or expand existing FMSs. The experts agreed that RMS (which can provide exactly the capacity and functionality needed, exactly when needed) is a desirable next step in the evolution of production systems. The key enabling technologies for RMS were identified as modular machines, open-architecture controls, high-speed machining, and methods, training and education for the operation of manufacturing systems.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46535/1/10845_2004_Article_397490.pd