Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess specific difficulties. Several reported procedures to isolate cell walls for proteomic analyses led to the isolation of a high proportion (more than 50%) of predicted intracellular proteins. The rationales of several published procedures to isolate cell walls for proteomics were analyzed, with regard to the bioinformatic-predicted subcellular localization of the identified proteins. A new procedure was developed to prepare cell walls from etiolated hypocotyls of Arabidopsis thaliana. After salt extraction, a high proportion of proteins predicted to be secreted was released (73%), belonging to the same functional classes as proteins identified using previously described protocols. The new cell wall preparation described in this paper gives the lowest proportion of proteins predicted to be intracellular when compared to available protocols. The application of its principles should lead to a more realistic view of the cell wall proteome, at least for the weakly bound CWP extractable by salts. In addition, it offers a clean cell wall preparation for subsequent extraction of strongly bound CWP

Studying the Boundary Layer Late Afternoon and Sunset Turbulence (BLLAST)

At the end of the afternoon, when the surface heat fluxes start to sharply decrease, the CBL turns from a convective well-mixed layer to an intermittently turbulent residual layer overlying a stably-stratified boundary layer. This transition raises several observational and modelling issues. Even the definition of the boundary layer during this period is fuzzy, since there is no consensus on what criteria to use and no simple scaling laws to apply. Yet it plays an important role in such diverse atmospheric phenomena as transport and diffusion of trace constituents or wind energy production. This phase of the diurnal cycle remains largely unexplored, partly due to the difficulty of measuring weak and intermittent turbulence, anisotropy, horizontal heterogeneity, and rapid time changes. The Boundary Layer Late Afternoon and Sunset Turbulence (BLLAST) project is gathering about thirty research scientists from the European Union and the United States to work on this issue. A field campaign (BLLAST-FE) is planned for spring or summer 2011 in Europe. BLLAST will utilize these observations, as well as previous datasets, large-eddy and direct numerical simulations, and mesoscale modelling to better understand the processes, suggest new parameterisations, and evaluate forecast models during this transitional period. We will present the issues raised by the late afternoon transition and our strategy to study it.Peer ReviewedPostprint (published version

Studying the Boundary Layer Late Afternoon nd Sunset Turbulence (BLLAST)

At the end of the afternoon, when the surface heat fluxes start to sharply decrease, the CBL turns from a convective well-mixed layer to an intermittently turbulent residual layer overlying a stably-stratified boundary layer. This transition raises several observational and modeling issues. Even the definition of the boundary layer during this period is fuzzy, since there is no consensus on what criteria to use and no simple scaling laws to apply. Yet it plays an important role in such diverse atmospheric phenomena as transport and diffusion of trace constituents or wind energy production. This phase of the diurnal cycle remains largely unexplored, partly due to the difficulty of measuring weak and intermittent turbulence, anisotropy, horizontal heterogeneity, and rapid time changes. The Boundary Layer Late Afternoon and Sunset Turbulence (BLLAST) project is gathering about thirty research scientists from the European Union and the United States to work on this issue. A field campaign (BLLAST-FE) is planned for spring or summer 2011 in Europe. BLLAST will utilize these observations, as well as previous datasets, large-eddy and direct numerical simulations, and mesoscale modeling to better understand the processes, suggest new parameterizations, and evaluate forecast models during this transitional period. We will present the issues raised by the late afternoon transition and our strategy to study it.Peer ReviewedPostprint (published version

Cell wall proteins: a new insight through proteomics

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins at the cell surface. The application of proteomic approaches to the cell wall compartment raises important questions: are there technical problems specific to cell wall proteomics? What kinds of proteins can be found in Arabidopsis walls? Are some of them unexpected? What sort of post-translational modifications have been characterized in cell wall proteins to date? The purpose of this review is to discuss the experimental results obtained to date using proteomics, as well as some of the new questions challenging future research

Etude fonctionnelle d'un récepteur lectine kinase (LecRK79) potentiel partenaire dans les contacts paroi-plasmalemme chez Arabidopsis thaliana

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Post-translational modifications of plant cell wall proteins and peptides: A survey from a proteomics point of view

International audienc