HEAD-EYE COORDINATION DURING A NATURAL TAPPING TASK

This thesis describes the research of Herst et al. (2001) who examined the coordination of head and eye movements while participants searched for targets under natural conditions in which the head was free to move. This investigation was done because, while the technical difficulties in measuring these patterns of head-eye coordination in humans have been overcome, the results obtained over the last 35 years have yet to show what the typical pattern of head-eye coordination looks like. Herst et al. (2001) described the `natural' temporal coordination of head and eye of four participants who tapped a sequence of targets arranged in 3D on a worktable in front of them. The results were not expected based on prior studies of head-eye coordination performed under less natural conditions. This thesis reinterprets the original (2001) findings and draws new conclusions in the light of new research on head-eye coordination conducted since then

Governance for a Changing Climate: Adapting Boston’s Built Environment for Increased Flooding

Climate Change is impacting everything in our society and in our world. The changes we are already experiencing are starting to multiply and accelerate. Determining how to respond to this new reality wisely within the governance and governmental structures that we have built is a complex challenge. Some might argue it is humankind’s greatest test. Given the monumental size of this task, it is difficult to simultaneously address all of the related issues both broadly and deeply. This is the third and final in a series of reports from the Sustainable Solutions Lab that were sponsored by the Boston Green Ribbon Commission with the generous support of the Barr Foundation. The goal of these reports was to build on the work done by the Climate Ready Boston process and explore select topics in more depth. This report takes a deep dive into a single issue: how the structure and tools of the local, regional, and state government can be modified and enhanced to minimize the impacts of climate changed-induced flooding (due to both sea level rise and increased precipitation) on Boston’s built environment. The goal here is to build on the two previous reports and help chart a path forward with both immediate next steps and transformational thinking

Governance for a Changing Climate: Adapting Boston's Built Environment for Increased Flooding

As this report indicates, implementing CRB is necessary but not sufficient to prepare Boston's built environment for the freshwater and coastal flooding anticipated to result from climate change. Additional steps we must take include reforming existing tools, monitoring and evaluating flood adaptation activities, and establishing governance for district-scale coastal flood protection implementation. This report presents an array of options for moving forward. Over the next year or so, the City and relevant stakeholders will need to come together and decide which, if any, of these options provide the best paths forward for a more resilient city and region.We recommend that the Governor of Massachusetts and the Mayor of Boston establish a joint commission to explore the options and determine a path forward. There is an opportunity for us to learn from the transition to clean energy as we prepare for climate change impacts. We recommend that the legislature take a leadership role in this effort as well, in order to evaluate the different options available to the Commonwealth as we attempt to address this dynamic challenge

Temporal coordination of the human head and eye during a natural sequential tapping task

AbstractThe ‘natural’ temporal coordination of head and eye was examined as four subjects tapped a sequence of targets arranged in 3D on a worktable in front of them. The head started to move before the eye 48% of the time. Both the head and eye started to move ‘simultaneously’ (within 8 ms of each other) 37% of the time. The eye started to move before the eye only 15% of the time. Gaze-shifts required to perform the tapping task were relatively large, 68% of them were between 27° and 57°. Gaze-shifts were symmetrical. There were almost as many lefts as rights. Very little inter- or intra-subject variability was observed. These results were not expected on the basis of prior studies of head/eye coordination performed under less natural conditions. They also were not expected given the results of two rather similar, relatively natural, prior experiments. We conclude that more observations under natural conditions will have to be made before we understand why, when and how human beings coordinate head and eyes as they perform everyday tasks in the work-a-day world

CLIMATE READY BOSTON: Climate Change and Sea Level Rise Projections for Boston, The Boston Research Advisory Group Report

This report summarizes the current understanding of the local factors that influence Boston’s future exposure to climate change risks. The following four risk factors were considered most relevant to Boston and are therefore evaluated in this report: sea-level rise, extreme precipitation, coastal storms and extreme temperatures. For each risk factor, a team of scientific experts, comprised of a team leader and three or more team members, was selected to evaluate and summarize the available information contained in both grey (reports, conference proceedings and the like) and peer-reviewed literature. Each team met independently between October 2015 and January 2016, and team leaders had regular teleconferences with the UMass Boston project team to keep them apprised of progress and to help overcome problems that were encountered. The process for reaching consensus is outlined in the next section

Isolation of anaerobic, extremely thermophilic, sulphur metabolising archaebacteria from New Zealand hot springs

Enrichments of New Zealand geo-thermal samples, initiated in anaerobic sulphur-containing media and incubated at temperatures above 85°C, yielded rod and coccal shaped organisms which possessed archaebacterial characteristics. Pure cultures were isolated and characterised. Five of the seven isolates, which were rod-shaped organisms and did not have an obligate requirement for sulphur respiration, were similar to Ther-moproteus sp. but had more neutral pH optima for growth. Three of these five Thermoproteus sp. were obligate heterotrophs, which has not previously been reported. The two coccal isolates had an obligate requirement for sulphur as an electron acceptor and were similar to Desulfurococcus sp. but again with more neutral pH optima for growth

A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells

SummaryConventional affinity purification followed by mass spectrometry (AP-MS) analysis is a broadly applicable method used to decipher molecular interaction networks and infer protein function. However, it is sensitive to perturbations induced by ectopically overexpressed target proteins and does not reflect multilevel physiological regulation in response to diverse stimuli. Here, we developed an interface between genome editing and proteomics to isolate native protein complexes produced from their natural genomic contexts. We used CRISPR/Cas9 and TAL effector nucleases (TALENs) to tag endogenous genes and purified several DNA repair and chromatin-modifying holoenzymes to near homogeneity. We uncovered subunits and interactions among well-characterized complexes and report the isolation of MCM8/9, highlighting the efficiency and robustness of the approach. These methods improve and simplify both small- and large-scale explorations of protein interactions as well as the study of biochemical activities and structure-function relationships

Residual OXPHOS is required to drive primary and metastatic lung tumours in an orthotopic breast cancer model

BackgroundFast adaptation of glycolytic and mitochondrial energy pathways to changes in the tumour microenvironment is a hallmark of cancer. Purely glycolytic ρ0 tumour cells do not form primary tumours unless they acquire healthy mitochondria from their micro-environment. Here we explored the effects of severely compromised respiration on the metastatic capability of 4T1 mouse breast cancer cells.Methods4T1 cell lines with different levels of respiratory capacity were generated; the Seahorse extracellular flux analyser was used to evaluate oxygen consumption rates, fluorescent confocal microscopy to assess the number of SYBR gold-stained mitochondrial DNA nucleoids, and the presence of the ATP5B protein in the cytoplasm and fluorescent in situ nuclear hybridization was used to establish ploidy. MinION nanopore RNA sequence analysis was used to compare mitochondrial DNA transcription between cell lines. Orthotopic injection was used to determine the ability of cells to metastasize to the lungs of female Balb/c mice.ResultsOXPHOS-deficient ATP5B-KO3.1 cells did not generate primary tumours. Severely OXPHOS compromised ρ0D5 cells generated both primary tumours and lung metastases. Cells generated from lung metastasis of both OXPHOS-competent and OXPHOS-compromised cells formed primary tumours but no metastases when re-injected into mice. OXPHOS-compromised cells significantly increased their mtDNA content, but this did not result in increased OXPHOS capacity, which was not due to decreased mtDNA transcription. Gene set enrichment analysis suggests that certain cells derived from lung metastases downregulate their epithelial-to-mesenchymal related pathways.ConclusionIn summary, OXPHOS is required for tumorigenesis in this orthotopic mouse breast cancer model but even very low levels of OXPHOS are sufficient to generate both primary tumours and lung metastases