Organogenesis and histological development of the wedge sole Dicologoglossa cuneata M. larva.

In this work, several features during the wedge sole larval development have been described. The newly hatched larva presented an acidophilic yolk with some oil drops. The digestive tract began to differentiate at 1 DAH, with a loop being discernible. The pancreas and liver were completely formed at 2 DAH, the former showing its typical basophilic acinar structure and acidophilic zymogen granules. The first supranuclear vesicles in enterocytes were seen at 3 DAH. At 4 DAH, yolk reserves were completely exhausted, the number of oesophagus and intestine mucous cells increased, and the heart was differentiated into four chambers: the venous sinus, atrium, ventricle, and arterious bulb. The development was fast and almost all organs were differentiated at 2 DAH. It is important to emphasize that gastric glands were not detected, a factor that should be considered when deciding diet formulation and feeding strategies for the rearing of this species This work was supported by Interreg Project 0251_ECOAQUA_5_E, financed by the EDRF (European Regional Development Fund).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua. &nbsp; La aced&iacute;a (Dicologoglossa cuneata) es un pez plano comercial objeto de investigaci&oacute;n en el IFAPA Agua del Pino. En este trabajo se describe su desarrollo larvario a nivel histomorfol&oacute;gico. La larva reci&eacute;n eclosionada presenta un saco vitelino con m&uacute;ltiples gotas de grasa, con el ano y la boca cerrados. A nivel histol&oacute;gico, se distingue el tubo digestivo como un segmento tubular recto situado por encima del vitelo. El h&iacute;gado y el p&aacute;ncreas ya est&aacute;n completamente formados a los 2-3 DDE (d&iacute;as despu&eacute;s de la eclosi&oacute;n), y el coraz&oacute;n se diferencia en atrio y ventr&iacute;culo. A los 4 DDE se reabsorbe el vitelo, la larva ya presenta pigmentaci&oacute;n y la boca y ano est&aacute;n abiertos; el tubo digestivo y el coraz&oacute;n se diferencian en 4 regiones, y se observa gran cantidad de t&uacute;bulos renales cerca de la vejiga natatoria. Este &oacute;rgano se insufla entre los 4 y 19 DDE y se atrofia a partir de 20 DDE, coincidiendo con el comienzo de la metamorfosis. El inicio de &eacute;sta se identifica por la transformaci&oacute;n de aleta tipo heterocerca a tipo homocerca, y por la migraci&oacute;n del ojo. La metamorfosis comienza a los 21-31 DDE y finaliza a los 25-37 DDE, por lo que existe una gran asincron&iacute;a. Este trabajo fue apoyado por el proyecto Interreg 0251_ECOAQUA_5_E , financiado por el FEDER (Fondo Europeo de Desarrollo Regional).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua. &nbsp;</p

Influence of stocking density on tissue lipid composition in flatfish Scophthalmus rhombus

The effects of the stocking density on the tissue lipid composition and biometric features of the brill were studied. Fish were cultured at three different stocking densities: 1; 5 and 15 Kg m-2 (LSD, MSD and HSD) during 5 weeks. Survival and several biometric, feeding and tissue lipid composition were assessed. Although final weight and specific growth rate decreased in higher densities, there were not significant differences between MSD and HSD. Differences for survival rate, feed efficiency, conversion index and feed intake were not detected among treatments. The minimum HSI was found in the HSD treatment, and condition factor varied inversely regards to stocking density. In liver, no differences among treatments were registered for total neutral lipids (TNL), though total polar lipids (TPL) was significantly higher in HSD. Muscle TPL did not vary significantly between LSD and HSD, but TNL was significantly higher in HSD. This work was supported by Interreg Project 0251_ECOAQUA_5_E, financed by the EDRF (European Regional Development Fund).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua. Se analiz&oacute; el efecto de la densidad de cultivo de parrachos en la composici&oacute;n lip&iacute;dica de diferentes tejidos de esta especie, as&iacute; como en la supervivencia y varios par&aacute;metros biom&eacute;tricos. Los peces se cultivaron durante 5 semanas a 3 densidades de cultivo: 1; 5 and 15 Kg m-2 (LSD, MSD y HSD). El peso final y la tasa de crecimiento espec&iacute;fica fueron menores en los cultivos a m&aacute;s densidad. Sin embargo no se observaron diferencias entre los grupos en supervivencia, eficiencia alimentaria, &iacute;ndice de conversi&oacute;n y tasa de ingesti&oacute;n. El &iacute;ndice hepatosom&aacute;tico fue menor los peces cultivados a m&aacute;s densidad. En el h&iacute;gado no se detectaron diferencias en los l&iacute;pidos neutros totales (TNL) entre los tratamientos, aunque la cantidad de l&iacute;pidos polares totales (TPL) fue mayor en HSD. En cambio los TPL fueron similares en el m&uacute;sculo en todos los tratamientos, mientras que los TNL fueron significativamente mayores en los peces cultivados a m&aacute;s densidad (HSD). Este trabajo fue apoyado por el proyecto Interreg 0251_ECOAQUA_5_E , financiado por el FEDER (Fondo Europeo de Desarrollo Regional).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.</p

Effects on growth of Dicologoglossa cuneata using two feeding strategies: feeding schedule and self-feeding.

The objective of this work was to study the capability of the wedge sole for using self-feeders and their daily feeding rhythm, comparing the growth between two different feeding strategies. The results showed that a week would be required to operated the self-feeding system correctly. Regarding the daily feeding rhythm, it was observed an abvious nocturnal activity . The SGR was higher in the shedule feeding treatment.This work was supported by Interreg Project 0251_ECOAQUA_5_E, financed by the EDRF (European Regional Development Fund). www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua. &nbsp;El objetivo de este trabajo fue estudiar la capacidad de la aced&iacute;a de usar comederos de autodemanda y su ritmo alimenticio diario, comparando en crecimiento entre dos estrategias alimenticias diferentes. Los resultados mostraron que las aced&iacute;as necesitaron una semana para activar los comederos correctamente. En cuanto al ritmo alimenticio diario, se observ&oacute; una clara actividad nocturna. La SGR fue m&aacute;s alta en la estrategia de alimentaci&oacute;n programada Este trabajo fue apoyado por el proyecto Interreg 0251_ECOAQUA_5_E , financiado por el FEDER (Fondo Europeo de Desarrollo Regional).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.</p

Effect of temperature on lipid consumption of brill (Scophthalmus rhombus L.) larvae.

The lipid composition and the bile salt-activated lipase (BAL) activity during the Scophthalmus rhombus larval rearing at 15 and 19 &ordm;C has been studied. Total and neutral lipid composition decreased in both treatments until 5 days after hatching (DAH), and increased from this time to 24 DAH. Polar lipid content (PL) remained constant between 0 and 5 DAH in 15 &ordm;C treatment, but decreased in 19 &ordm;C treatment. Saturated, monounsaturated and polyunsaturated fatty acids decreased in both groups until 5 DAH, and they after increased to 24 DAH. Most of fatty acid showed a similar trend including EPA and DHA. It is also remarkable that 16:1 n7, 18:0 and ARA content kept constant until 5 DAH in larvae cultured at 15 &ordm;C. There was an increase in the activity of BAL at 2 DAH for both treatments. This work was supported by Interreg Project 0251_ECOAQUA_5_E, financed by the EDRF (European Regional Development Fund). www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.. Se estudi&oacute; la composici&oacute;n lip&iacute;dica y la actividad de la lipase BAL durante el cultivo larvario de Scophthalmus rhombus a 15 y 19&ordm;C. La composici&oacute;n en l&iacute;pidos totales y neutros decreci&oacute; en ambos tratamientos hasta 5 d&iacute;as despu&eacute;s de la eclosi&oacute;n (dde), y se increment&oacute; desde entonces hasta 24 dde. El contenido en l&iacute;pidos polares (PL) permanecieron constantes entre 0 y 5 dde en las larvas cultivadas a 15&ordm;C, pero decrecieron en las cultivadas a 19&ordm;C. Los valores de &aacute;cidos grasos saturados, monoinsaturados y poliinsaturados decrecieron en ambos grupos hasta 5 dde, y despu&eacute;s se incrementaron hasta 24 dde. La mayor&iacute;a de &aacute;cidos grasos mostraron un comportamiento similar en ambos tratamientos, incluyendo EPA y DHA. Hay que destacar que el contenido en los &aacute;cidos grasos 16:1 n7, 18:0 y ARA permaneci&oacute; constante en s&oacute;lo en las larvas cultivadas a 15&ordm;C. En cuanto a la lipasa BAL, se detect&oacute; un incremento en su actividad en ambos tratamientos a 2 dde. Este trabajo fue apoyado por el proyecto Interreg 0251_ECOAQUA_5_E , financiado por el FEDER (Fondo Europeo de Desarrollo Regional).www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.. </p

Effect of inclusion of additives nutraceuticals in the resistance of juvenile Senegal sole (Solea senegalensis Kaup 1858) against experimental infections with Photobacterium damselae subsp. piscicida (A321) and Vibrio harveyi (a91)

In sole industrial culture, high mortality occurs that hinders the production of this species. Adequate nutrition must improve growth and resistance against fish pathogens. The aim of the study was to assess What effect does the inclusion of nutraceutical additives in resistance of juvenile Senegalese sole against common pathogens such as Photobacterium damselae subsp. piscicida (A321) and Vibrio harveyi (a91). Three experimental feeds were tested (E1 with probiotics, E2 with nucleotides and probiotic and E3 with probiotics, nucleotides and glucans) and a commercial (C). Significant differences were found in fish growth (E3 = E2&gt; E1&gt; C). It was also found a significant improvement in the survival of fish against experimental infection with pathogenic bacteria.This work was supported by Interreg Project 0251_ECOAQUA_5_E, financed by the EDRF (European Regional Development Fund). www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.En el cultivo del lenguado a nivel industrial se produce una alta mortalidad que dificulta la producci&oacute;n de esta especie. Una nutrici&oacute;n adecuada debe mejorar el crecimiento y la resistencia de los peces frente a pat&oacute;genos. El objetivo del estudio fue evaluar qu&eacute; efecto tiene la inclusi&oacute;n de aditivos nutrace&uacute;ticos en la resistencia de juveniles de lenguado senegal&eacute;s frente a pat&oacute;genos habituales como Photobacterium damselae subsp. piscicida (a321) y Vibrio harveyi (a91). Se probaron tres piensos experimentales ( E1 con probi&oacute;ticos, E2 con probi&oacute;ticos y nucle&oacute;tidos y E3 con probi&oacute;ticos, nucle&oacute;tidos y glucanos) y uno comercial (C). Se encontraron diferencias significativas en el crecimiento de los peces (E3 = E2 &gt; E1 &gt; C). Adem&aacute;s se encontr&oacute; una mejora considerable en la supervivencia de los peces frente a una infecci&oacute;n experimental con las bacterias pat&oacute;genas.Este trabajo fue apoyado por el proyecto Interreg 0251_ECOAQUA_5_E , financiado por el FEDER (Fondo Europeo de Desarrollo Regional). www.juntadeandalucia.es/agriculturaypesca/ifapa/ecoaqua.</p