167 research outputs found
Characterization of Posidonia Oceanica Seagrass Aerenchyma through Whole Slide Imaging: A Pilot Study
Characterizing the tissue morphology and anatomy of seagrasses is essential
to predicting their acoustic behavior. In this pilot study, we use histology
techniques and whole slide imaging (WSI) to describe the composition and
topology of the aerenchyma of an entire leaf blade in an automatic way
combining the advantages of X-ray microtomography and optical microscopy.
Paraffin blocks are prepared in such a way that microtome slices contain an
arbitrarily large number of cross sections distributed along the full length of
a blade. The sample organization in the paraffin block coupled with whole slide
image analysis allows high throughput data extraction and an exhaustive
characterization along the whole blade length. The core of the work are image
processing algorithms that can identify cells and air lacunae (or void) from
fiber strand, epidermis, mesophyll and vascular system. A set of specific
features is developed to adequately describe the convexity of cells and voids
where standard descriptors fail. The features scrutinize the local curvature of
the object borders to allow an accurate discrimination between void and cell
through machine learning. The algorithm allows to reconstruct the cells and
cell membrane features that are relevant to tissue density, compressibility and
rigidity. Size distribution of the different cell types and gas spaces, total
biomass and total void volume fraction are then extracted from the high
resolution slices to provide a complete characterization of the tissue along
the leave from its base to the apex
A sequential Bayesian approach to vertical slice tomography of a shallow water environment
Adjointâbased control of nonlocal boundary conditions for Claerboutâs wideâangle parabolic approximation
On the usefulness of waterborne measurement of particle velocity in geoacoustic inversion
Characterization of sediment dynamics in an estuary environment using acoustic techniques
Geoacoustic inversion in the frequency range 0.8â1.6 kHz with drifting sparse arrays during MREA/BP'07 experiment
- âŚ