513 research outputs found

    A preliminary list of the Trichoptera of British Columbia

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    CHROMOSOME POLYPLOIDIZATION IN HUMAN LEUKOCYTES INDUCED BY RADIATION

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    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73203/1/j.1749-6632.1969.tb50297.x.pd

    The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing

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    Packed human erythrocytes were frozen in liquid nitrogen using hydroxyethyl starch as a cryoprotective agent. Upon thawing, the cells were washed with a balanced salt solution. The washed cells were stable when resuspended in 0.154 n NaCl. The postthaw saline stability approximated the total recovery after washing the cells. Intracellular potassium loss was 15% immediately postthaw, and an additional 15% loss occurred after washing. These results indicate that HES can be removed from frozen-thawed cells by washing, and that freezing erythrocytes protected by HES does not result in significant losses of intracellular potassium.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33516/1/0000014.pd

    Large unit red cell cryopreservation with hydroxyethyl starch

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    Full units of red blood cells frozen with 14% hydroxyethyl starch (HES) yield cell recoveries near 97% and saline stabilities greater than 80%. Potassium leaves the cells during the freeze-thaw cycle and increases the extracellular concentration of this ion to near 35 meq/l. Unwashed cells (those with plasma present) and saline washed cells yield similar results. Storage of the thawed red cells at 4 [deg]C for up to 48 hr causes little change in the cells.Examination by electron microscopy of samples from thawed units reveals some red cells with portions of their membrane missing. We believe this represents damage from the freeze-thaw cycle and also that all free supernatant hemoglobin does not arise from completely lysed cells.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/21672/1/0000060.pd

    Red cells preserved with 10% hydroxyethyl starch: Effect of prefreeze washing

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    Prefreeze washing of red cells with saline and other wash solutions before freezing with 10% hydroxyethyl starch does not yield an improved product after thawing. The saline stabilities and cell recoveries are reduced and the level of supernatant hemoglobin increased. These data further support the use of 14% as the most acceptable concentration of hydroxyethyl starch for cryopreservation of red cells.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/21984/1/0000394.pd

    Cytogenetic Studies of Normal and Cleft-Palate Epitheliums in Mice

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    This study investigated teratogenic activity on a cellular level through analysis of the cytogenetic complex in cleft-palate epithelial cells from the progenies of mice treated with cortisone. Analysis showed no detectable aberration from a normal chromosomal complement.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68120/2/10.1177_00220345690480041601.pd

    Viable vein graft preservation

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    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22115/1/0000542.pd
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