Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase

The MCM gene from the archaeon Halobacterium, with and without its intein, was cloned into an Escherichia coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the intein, could be detected

Accurate determination of orientation relationship between ferrite and (Fe, Mn)(3)AlC in an Fe-Mn-Al-C eutectoid alloy

The orientation relationship between ferrite and (Fe,Mn)(3)AlC has been investigated in an Fe-3.5Mn-5.8Al-1.05C based eutectoid alloy. Electron diffraction pattern analysis shows two possible orientation relationships between both phases. Accurate analysis using convergent beam electron diffraction reveals the deviation of [001](ferrite) pole from [110]((Fe,Mn)3AlC) pole in Nishiyama-Wasserman orientation relationship and the coincidence of [111](ferrite) and [110] ((Fe,Mn)3AlC) poles in Kurdjumov-Sachs orientation relationship. Transmission Kikuchi diffraction resolves the orientation of several tens nanometer scales of ferrite +(Fe,Mn)(3)AlC lamellar. Kurdjumov-Sachs orientation relationship between ferrite and (Fe,Mn)(3)AlC is clarified by the comparison of deviation angle from both orientation relationships. Coherency at the interphase boundaries is further discussed. (C) 2020 Elsevier B.V. All rights reserved.11Nsciescopu