Insight into Biochemical Characterization of Plant Sesquiterpene Synthases

A fast and reproducible protocol was established for enzymatic characterization of plant sesquiterpene synthases that can incorporate radioactivity in their products. The method utilizes the 96-well format in conjunction with cluster tubes and enables processing of >200 samples a day. Along with reduced reagent usage, it allows further reduction in the use of radioactive isotopes and flammable organic solvents. The sesquiterpene synthases previously characterized were expressed in yeast, and the plant-derived Thapsia garganica kunzeaol synthase TgTPS2 was tested in this method. K M for TgTPS2 was found to be 0.55 μM; the turnover number, k cat , was found to be 0.29 s −1 , k cat for TgTPS2 is in agreement with that of terpene synthases of other plants, and k cat / K M was found to be 0.53 s −1 μM −1 for TgTPS2. The kinetic parameters were in agreement with previously published data

Developments and Perspectives in Bryophyte Biotechnology in Sub-Saharan Africa

The work described here covers an examination of new bioproducts based on sub-Saharan bryophytes. The work includes in vitro testing of extracts from moss and liverworts against plant pathogenic microbes causing food decay and field crop losses. Additionally, we have shown specific antimicrobial activities of Marchantia debilis and moss against Erwinia spp and Pseudomonas spp. The extracts were also tested against aflatoxin-producing fungi isolated from food crops such as maize and peanuts. The efficacy of the extracts on clinical dermatological fungal isolates like Dermatophilus congolensis has not been reported. This led to the production of an antifungal solution of bryophyte extracts, which was tested in vivo on animals with skin diseases caused by Dermatophilosis. Around 99.5% of the animals were treated. The antifungal solution for treatments has been labeled Bryosol, while the disinfectants solution is labeled Bryo-disinfectants and the crop-fungicide is labeled Bryo-fungicides. A mini field pilot trial with Bryo-fungicide showed that crops infected with pathogenic fungi were treated. The results provide the first attempt to demonstrate the use of bioproducts for organic treatment of agricultural crops and diseases in animals based on sub-Saharan bryophytes

Mediatised human rights education: the (challenging) role of the Norwegian Broadcasting Corporation

The purpose of this article is to analyse and discuss Selma and the Quest for the Perfect Faith, a TV series made by The Norwegian Broadcasting Corporation and promoted for use in schools, and the accompanying teaching material about freedom of religion made by Save the Children. We discuss the series and material critically from a human rights and a human rights education perspective, and evaluate their suitability for use in religious education. The article is informed by mediatisation theory and argues that freedom of religion is primarily operationalised in accordance with journalistic criteria for presenting religion, and that it does not sufficiently balance the rights of children and the liberty of parents. This is inconsistent with sound human rights education and highlights the need for critical awareness when operationalising educational material produced and distributed by media actors for use in the classroom.publishedVersio