Climate variability in SE Europe since 1450 AD based on a varved sediment record from Etoliko Lagoon (Western Greece)

To achieve deeper understanding of climate variability during the last millennium in SE Europe, we report new sedimentological and paleoecological data from Etoliko Lagoon, Western Greece. The record represents the southernmost annually laminated (i.e., varved) archive from the Balkan Peninsula spanning the Little Ice Age, allowing insights into critical time intervals of climate instability such as during the Maunder and Dalton solar minima. After developing a continuous, ca. 500-year-long varve chronology, high-resolution μ–XRF counts, stable-isotope data measured on ostracod shells, palynological (including pollen and dinoflagellate cysts), and diatom data are used to decipher the season-specific climate and ecosystem evolution at Etoliko Lagoon since 1450 AD. Our results show that the Etoliko varve record became more sensitive to climate change from 1740 AD onwards. We attribute this shift to the enhancement of primary productivity within the lagoon, which is documented by an up to threefold increase in varve thickness. This marked change in the lagoon's ecosystem was caused by: (i) increased terrestrial input of nutrients, (ii) a closer connection to the sea and human eutrophication particularly from 1850 AD onwards, and (iii) increasing summer temperatures. Integration of our data with those of previously published paleolake sediment records, tree-ring-based precipitation reconstructions, simulations of atmospheric circulation and instrumental precipitation data suggests that wet conditions in winter prevailed during 1740–1790 AD, whereas dry winters marked the periods 1790–1830 AD (Dalton Minimum) and 1830–1930 AD, the latter being sporadically interrupted by wet winters. This variability in precipitation can be explained by shifts in the large-scale atmospheric circulation patterns over the European continent that affected the Balkan Peninsula (e.g., North Atlantic Oscillation). The transition between dry and wet phases at Etoliko points to longitudinal shifts of the precipitation pattern in the Balkan Peninsula during the Little Ice Age

Genetic engineering approaches for the fermentative production of phenylglycines

L-phenylglycine (L-Phg) is a rare non-proteinogenic amino acid, which only occurs in some natural compounds, such as the streptogramin antibiotics pristinamycin I and virginiamycin S or the bicyclic peptide antibiotic dityromycin. Industrially, more interesting than L-Phg is the enantiomeric D-Phg as it plays an important role in the fine chemical industry, where it is used as a precursor for the production of semisynthetic β-lactam antibiotics. Based on the natural L-Phg operon from Streptomyces pristinaespiralis and the stereo-inverting aminotransferase gene hpgAT from Pseudomonas putida, an artificial D-Phg operon was constructed. The natural L-Phg operon, as well as the artificial D-Phg operon, was heterologously expressed in different actinomycetal host strains, which led to the successful production of Phg. By rational genetic engineering of the optimal producer strains S. pristinaespiralis and Streptomyces lividans, Phg production could be improved significantly. Here, we report on the development of a synthetic biology-derived D-Phg pathway and the optimization of fermentative Phg production in actinomycetes by genetic engineering approaches. Our data illustrate a promising alternative for the production of Phgs.Deutsche ForschungsgemeinschaftBaden-Württemberg-StiftungDeutsches Zentrum für InfektionsforschungProjekt DEA

Challenging old microbiological treasures for natural compound biosynthesis capacity

Strain collections are a treasure chest of numerous valuable and taxonomically validated bioresources. The Leibniz Institute DSMZ is one of the largest and most diverse microbial strain collections worldwide, with a long tradition of actinomycetes research. Actinomycetes, especially the genus Streptomyces, are renowned as prolific producers of antibiotics and many other bioactive natural products. In light of this, five Streptomyces strains, DSM 40971T, DSM 40484T, DSM 40713T, DSM 40976T, and DSM 40907T, which had been deposited a long time ago without comprehensive characterization, were the subject of polyphasic taxonomic studies and genome mining for natural compounds based on in vitro and in silico analyses. Phenotypic, genetic, and phylogenomic studies distinguished the strains from their closely related neighbors. The digital DNA–DNA hybridization and average nucleotide identity values between the five strains and their close, validly named species were below the threshold of 70% and 95%–96%, respectively, determined for prokaryotic species demarcation. Therefore, the five strains merit being considered as novel Streptomyces species, for which the names Streptomyces kutzneri sp. nov., Streptomyces stackebrandtii sp. nov., Streptomyces zähneri sp. nov., Streptomyces winkii sp. nov., and Streptomyces kroppenstedtii sp. nov. are proposed. Bioinformatics analysis of the genome sequences of the five strains revealed their genetic potential for the production of secondary metabolites, which helped identify the natural compounds cinerubin B from strain DSM 40484T and the phosphonate antibiotic phosphonoalamide from strain DSM 40907T and highlighted strain DSM 40976T as a candidate for regulator-guided gene cluster activation due to the abundance of numerous “Streptomyces antibiotic regulatory protein” (SARP) genes

Near-Infrared Absorbance of Single-Walled Carbon Nanotubes Dispersed in Dimethylformamide

Absorption spectra of single-walled carbon nanotubes (SWNT) dispersed in dimethylformamide (DMF) have been recorded in the visible to near-infrared range. Besides broad absorption bands, the spectra show a sharp peak at 5187 cm-1, which has been attributed in the literature to the absorption of semiconducting nanotubes with a band gap of 0.64 eV [Ausman et al. J. Phys. Chem. B 2000, 104, 8911]. On the basis of absorbance measurements for samples that show the sharp absorbance peak but do not contain SWNTs, we conclude that the origin of the peak at 5187 cm-1 is not due to semiconducting nanotubes but due to water present in the SWNT/DMF suspension. A comparative study of D2O in DMF reproduces a similar peak that is isotope-shifted into the near-infrared