Messung der Einkopplung statistischer elektromagnetischer Felder in eine verdrillte Doppelleitung mit angepasstem Leitungsabschluss

In vielen Bereichen der Kommunikationstechnik werden verdrillte Leitungen eingesetzt, da diese gegenüber externen elektromagnetischen Feldern eine höhere Störfestigkeit ermöglichen. Aus Kostenund Gewichtsgründen werden solche Leitungen häufig ungeschirmt ausgeführt. Ist vom einfallenden Feld nicht die exakte Einfallsrichtung und Polarisation, sondern nur eine gewisse Verteilung und räumliche Korrelation der Feldgrößen bekannt, so spricht man auch von statistischen Feldern. Solche Felder haben gerade in resonanten Umgebungen eine hohe Praxisrelevanz. Sie können für experimentelle Untersuchungen in Modenverwirbelungskammern erzeugt werden. Im Beitrag wird die Messung der Einkopplung statistischer Felder in eine verdrillte Doppelleitung im Freiraum beschrieben. Ein entsprechendes Simulationsmodell [5] ist bereits vorhanden und kann damit experimentell validiert werden. Ähnliche Messungen wurden bereits bei einer Anregung durch einzelne ebene Wellen mit veränderlicher Einfallsrichtung [1] durchgeführt. Auch für die Anregung durch statistische Felder existieren bereits Messergebnisse [4], allerdings mit einer leerlaufenden bzw. kurzgeschlossenen Leitung. In diesem Beitrag wird eine Leitung mit angepasstem Leitungsabschluss untersucht. Am angepassten Leitungsende treten nahezu keine Reflexionen und damit auch keine Mehrfachreflexionen auf. Deshalb gibt es nur sehr geringe Leitungsresonanzen und der Einfluss der Schlaglänge der Verdrillung lässt sich aus der Frequenzabhängigkeit der eingekoppelten Spannung einfacher ermitteln

Nuclear and nucleon transitions of the H di-baryon

We consider 3 types of processes pertinent to the phenomenology of an H di-baryon: conversion of two $\Lambda$'s in a doubly-strange hypernucleus to an H, decay of the H to two baryons, and -- if the H is light enough -- conversion of two nucleons in a nucleus to an H. We compute the spatial wavefunction overlap using the Isgur-Karl and Bethe-Goldstone wavefunctions, and treat the weak interactions phenomenologically. The observation of $\Lambda$ decays from doubly-strange hypernuclei puts a constraint on the H wavefunction which is plausibly satisfied. In this case the H is very long-lived as we calculate. An absolutely stable H is not excluded at present. SuperK can provide valuable limits

Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: Considerations on process development

Controlled large-scale production of human pluripotent stem cells (hPSCs) is indispensable for their envisioned clinical translation. Aiming at advanced process development in suspension culture, the sensitivity of hPSC media to continuous peristaltic pump-based circulation, a well-established technology extensively used in hydraulically-driven bioreactors, was investigated. Unexpectedly, conditioning of low protein media (i.e. E8 and TeSR-E8) in a peristaltic pump circuit induced severe viability loss of hPSCs cultured as aggregates in suspension. Optical, biochemical, and cytological analyses of the media revealed that the applied circulation mode resulted in the reduction of the growth hormone insulin by precipitation of micro-sized particles. Notably, in contrast to insulin depletion, individual withdrawal of other medium protein components (i.e. bFGF, TGFβ1 or transferrin) provoked minor reduction of hPSC viability, if any. Supplementation of the surfactant glycerol or the use of the insulin analogue Aspart did not overcome the issue of insulin precipitation. In contrast, the presence of bovine or human serum albumin (BSA or HSA, respectively) stabilized insulin rescuing its content, possibly by acting as molecular chaperone-like protein, ultimately supporting hPSC maintenance. This study highlights the potential and the requirement of media optimization for automated hPSC processing and has broad implications on media development and bioreactor-based technologies. © 2017 The Author(s).Horizon 2020/Marie Skłodowska-Curie Individual Fellowship POSEIDONDFG/EXC/REBIRTHDFG/EXC62/3DFG/ZW64/4-1BMBF/13N12606BMBF/13N14086StemBANCCH2020/TECHNOBEATHannover Medical School internal program (HiLF)Joachim Herz Stiftun

Aphanomyces-Resistant Alfalfa: A Solution to a Common Problem in Spring Seedings

For several decades, farmers have experienced a common stand-establishment disease syndrome when spring-seeded alfalfa was followed by extended periods of wet weather. Seedlings affected by this syndrome exhibit severe stunting as well as yellowing and reddening of seed leaves (cotyledons), but they do not wilt or collapse, as they might from a damping-off disease. Commonly, the problem affects most or all of the field. Based on research that began in the 1980\u27s, we suspected that a fungus called Aphanomyces euteiches (hereafter simply called Aphanomyces) was responsible. This root-rot fungus can be found in the majority of alfalfa fields we have sampled in central and western Kentucky. However, for many years we lacked conclusive proof that Aphanomyces was, in fact, the cause of this common problem in spring-seeded alfalfa. We also did not have rigorous proof that the syndrome could be avoided by sowing Aphanomyces-resistant alfalfa varieties, which started becoming commercially available in the early 1990\u27s. In this report, we provide a brief summary of research to support our new recommendation: that spring-seeded alfalfa should be sown only with varieties having an R or HR rating to Aphanomyces root rot (ARR)

MT1-matrix metalloproteinase directs arterial wall invasion and neointima formation by vascular smooth muscle cells

During pathologic vessel remodeling, vascular smooth muscle cells (VSMCs) embedded within the collagen-rich matrix of the artery wall mobilize uncharacterized proteolytic systems to infiltrate the subendothelial space and generate neointimal lesions. Although the VSMC-derived serine proteinases, plasminogen activator and plasminogen, the cysteine proteinases, cathepsins L, S, and K, and the matrix metalloproteinases MMP-2 and MMP-9 have each been linked to pathologic matrix-remodeling states in vitro and in vivo, the role that these or other proteinases play in allowing VSMCs to negotiate the three-dimensional (3-D) cross-linked extracellular matrix of the arterial wall remains undefined. Herein, we demonstrate that VSMCs proteolytically remodel and invade collagenous barriers independently of plasmin, cathepsins L, S, or K, MMP-2, or MMP-9. Instead, we identify the membrane-anchored matrix metalloproteinase, MT1-MMP, as the key pericellular collagenolysin that controls the ability of VSMCs to degrade and infiltrate 3-D barriers of interstitial collagen, including the arterial wall. Furthermore, genetic deletion of the proteinase affords mice with a protected status against neointimal hyperplasia and lumen narrowing in vivo. These studies suggest that therapeutic interventions designed to target MT1-MMP could prove beneficial in a range of human vascular disease states associated with the destructive remodeling of the vessel wall extracellular matrix

Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells

In vitro differentiation of human pluripotent stem cells (hPSCs) recapitulates early aspects of human embryogenesis, but the underlying processes are poorly understood and controlled. Here we show that modulating the bulk cell density (BCD: cell number per culture volume) deterministically alters anteroposterior patterning of primitive streak (PS)-like priming. The BCD in conjunction with the chemical WNT pathway activator CHIR99021 results in distinct paracrine microenvironments codifying hPSCs towards definitive endoderm, precardiac or presomitic mesoderm within the first 24 h of differentiation, respectively. Global gene expression and secretome analysis reveals that TGFß superfamily members, antagonist of Nodal signalling LEFTY1 and CER1, are paracrine determinants restricting PS progression. These data result in a tangible model disclosing how hPSC-released factors deflect CHIR99021-induced lineage commitment over time. By demonstrating a decisive, functional role of the BCD, we show its utility as a method to control lineage-specific differentiation. Furthermore, these findings have profound consequences for inter-experimental comparability, reproducibility, bioprocess optimization and scale-up.DFG/REBIRTHDFG/EXC62/1DFG/ZW 64/4-1DFG/MA 2331/16-1BMBF/13N12606BMBF/StemBANCCEU H2020/66872

Comparison of intramyocellular lipid metabolism in patients with diabetes and male athletes

Contributions D.D., A.H., S.G., S.P. and M.D. conceived the study and together with L.v.L., G.L., F.T. obtained the grant funding. AM executed the patient screening, recruitment, intervention planning, carried out all study investigations under respective specialist supervision (A.H./D.C./D.D. for magnetic resonance spectroscopy, F.T./G.L./D.D. for stable isotope investigation, S.G. for exercise intervention, S.P. for clinical supervision/management of diabetes as required, M.D. for all molecular laboratory analyses, A.M. analysed all data and performed statistical analysis under the supervision of G.H. L.v.L. provided expert advice in athletic physiology. Lipidomic analyses were carried out in P.W. laboratory. Blood/skeletal muscle enrichment analyses were carried out in B.F./F.T.-G.L. laboratories respectively, with practical input from R.G. A.R. and L.C. contributed as overall help to deliver study assessments in a technical role. M.K.H. analysed the food diaries. D.E.N. contributed to manuscript writing. D.D. and M.D. were the PhD supervisors for A.M. whose PhD thesis was based on this work. All authors contributed their respective specialist sections in drafting the manuscript.Peer reviewe

Reactive microglia are the major source of tumor necrosis factor alpha and contribute to astrocyte dysfunction and acute seizures in experimental temporal lobe epilepsy.

Extensive microglia reactivity has been well described in human and experimental temporal lobe epilepsy (TLE). To date, however, it is not clear whether and based on which molecular mechanisms microglia contribute to the development and progression of focal epilepsy. Astroglial gap junction coupled networks play an important role in regulating neuronal activity and loss of interastrocytic coupling causally contributes to TLE. Here, we show in the unilateral intracortical kainate (KA) mouse model of TLE that reactive microglia are primary producers of tumor necrosis factor (TNF)α and contribute to astrocyte dysfunction and severity of status epilepticus (SE). Immunohistochemical analyses revealed pronounced and persistent microglia reactivity, which already started 4 h after KA-induced SE. Partial depletion of microglia using a colony stimulating factor 1 receptor inhibitor prevented early astrocyte uncoupling and attenuated the severity of SE, but increased the mortality of epileptic mice following surgery. Using microglia-specific inducible TNFα knockout mice we identified microglia as the major source of TNFα during early epileptogenesis. Importantly, microglia-specific TNFα knockout prevented SE-induced gap junction uncoupling in astrocytes. Continuous telemetric EEG recordings revealed that during the first 4 weeks after SE induction, microglial TNFα did not significantly contribute to spontaneous generalized seizure activity. Moreover, the absence of microglial TNFα did not affect the development of hippocampal sclerosis but attenuated gliosis. Taken together, these data implicate reactive microglia in astrocyte dysfunction and network hyperexcitability after an epileptogenic insult

Towards Highly Automated Driving: Intermediate report on the HAVEit-Joint System

International audienceThis overview article describes the goals, concepts and very preliminary results of the subproject Joint System within the EU-project HAVEit. The goal of HAVEit is to develop and investigate vehicle automation beyond ADAS systems, especially highly automated driving, where the automation is doing a high percentage of the driving, while the driver is still meaningfully involved in the driving task. In HAVEit, an overarching architecture and several prototypes will be built up over time by manufacturers and suppliers. As a trail blazer, a Joint System prototype is under development by an interdisciplinary team of several European research institutes in order to investigate and demonstrate the basic principles of highly automated driving, which will then be gradually applied to vehicles closer to serial production. Starting with sensor data fusion, the Co-System part of the Joint Systems plans manoeuvres and trajectories, which are then used to control active interfaces and, taking into account the results of an online driver assessment, joined with the actions of the driver. While many aspects of this research undertaking are still under investigation, the concept, a first prototype and first results from a simulator evaluation will be sketched