Thon rings from amorphous ice and implications of beam-induced Brownian motion in single particle electron cryo-microscopy

We have recorded dose-fractionated electron cryo-microscope images of thin films of pure flash-frozen amorphous ice and pre-irradiated amorphous carbon on a Falcon~II direct electron detector using 300 keV electrons. We observe Thon rings \cite{Thon1966} in both the power spectrum of the summed frames and the sum of power spectra from the individual frames. The Thon rings from amorphous carbon images are always more visible in the power spectrum of the summed frames whereas those of amorphous ice are more visible in the sum of power spectra from the individual frames. This difference indicates that while pre-irradiated carbon behaves like a solid during the exposure, amorphous ice behaves like a fluid with the individual water molecules undergoing beam-induced motion. Using the measured variation in the power spectra amplitude with number of electrons per image we deduce that water molecules are randomly displaced by mean squared distance of $\sim$ 1.1 \AA$^{2}$ for every incident 300 keV e$^{-}$/\AA$^2$. The induced motion leads to an optimal exposure with 300 keV electrons of 4.0 e$^{-}$/\AA$^2$ per image with which to see Thon rings centred around the strong 3.7{\AA} scattering peak from amorphous ice. The beam-induced movement of the water molecules generates pseudo-Brownian motion of embedded macromolecules. The resulting blurring of single particle images contributes an additional term, on top of that from radiation damage, to the minimum achievable B-factor for macromolecular structure determination.Comment: 11 pages, 6 figures, Supplementary information 6 pages with 5 figure

Extent of regretted sexual intercourse among young teenagers in Scotland: a cross sectional survey

No abstract available

Experimental Study of the Role of Atomic Interactions on Quantum Transport

We report an experimental study of quantum transport for atoms confined in a periodic potential and compare between thermal and BEC initial conditions. We observe ballistic transport for all values of well depth and initial conditions, and the measured expansion velocity for thermal atoms is in excellent agreement with a single-particle model. For weak wells, the expansion of the BEC is also in excellent agreement with single-particle theory, using an effective temperature. We observe a crossover to a new regime for the BEC case as the well depth is increased, indicating the importance of interactions on quantum transport.Comment: 4 pages, 3 figure

Yang-Mills Theory on a Cylinder Coupled to Point Particles

We study a model of quantum Yang-Mills theory with a finite number of gauge invariant degrees of freedom. The gauge field has only a finite number of degrees of freedom since we assume that space-time is a two dimensional cylinder. We couple the gauge field to matter, modeled by either one or two nonrelativistic point particles. These problems can be solved {\it without any gauge fixing}, by generalizing the canonical quantization methods of Ref.\[rajeev] to the case including matter. For this, we make use of the geometry of the space of connections, which has the structure of a Principal Fiber Bundle with an infinite dimensional fiber. We are able to reduce both problems to finite dimensional, exactly solvable, quantum mechanics problems. In the case of one particle, we find that the ground state energy will diverge in the limit of infinite radius of space, consistent with confinement. In the case of two particles, this does not happen if they can form a color singlet bound state (`meson').Comment: 37 pages, UR-1327 ER-40685-77

Multiparticle Schrodinger operators with point interactions in the plane

We study a system of N bosons in the plane interacting with delta function potentials. After a coupling constant renormalization we show that the Hamiltonian defines a self-adjoint operator and obtain a lower bound for the energy. The same results hold if one includes a regular inter-particle potential.Comment: 17 pages, Late

Experimental demonstration of painting arbitrary and dynamic potentials for Bose-Einstein condensates

There is a pressing need for robust and straightforward methods to create potentials for trapping Bose-Einstein condensates which are simultaneously dynamic, fully arbitrary, and sufficiently stable to not heat the ultracold gas. We show here how to accomplish these goals, using a rapidly-moving laser beam that "paints" a time-averaged optical dipole potential in which we create BECs in a variety of geometries, including toroids, ring lattices, and square lattices. Matter wave interference patterns confirm that the trapped gas is a condensate. As a simple illustration of dynamics, we show that the technique can transform a toroidal condensate into a ring lattice and back into a toroid. The technique is general and should work with any sufficiently polarizable low-energy particles.Comment: Minor text changes and three references added. This is the final version published in New Journal of Physic

Bose-Einstein Condensate Driven by a Kicked Rotor in a Finite Box

We study the effect of different heating rates of a dilute Bose gas confined in a quasi-1D finite, leaky box. An optical kicked-rotor is used to transfer energy to the atoms while two repulsive optical beams are used to confine the atoms. The average energy of the atoms is localized after a large number of kicks and the system reaches a nonequilibrium steady state. A numerical simulation of the experimental data suggests that the localization is due to energetic atoms leaking over the barrier. Our data also indicates a correlation between collisions and the destruction of the Bose-Einstein condensate fraction.Comment: 7 pages, 8 figure

Framing Expectations in Early HIV Cure Research

Language used to describe clinical research represents a powerful opportunity to educate volunteers. In the case of HIV cure research there is an emerging need to manage expectations by using the term ‘experiment’. Cure experiments are proof-of-concept studies designed to evaluate novel paradigms to reduce persistent HIV-1 reservoirs, without any expectation of medical benefit