11 research outputs found

    Detection of heat-stable enterotoxin from escherichia coli by ELISA based on monoclonal antibody

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    SCOPUS: le.jinfo:eu-repo/semantics/publishe

    Possible field test for st-producing escherichia coli

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    SCOPUS: le.jinfo:eu-repo/semantics/publishe

    CRYPTOSPORIDIUM RELATED TO MEASLES DIARRHOEA IN RWANDA

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    SCOPUS: le.jinfo:eu-repo/semantics/publishe

    ENTEROPATHOGENIC AGENTS IN CHILDREN WITH DIARRHOEA IN RURAL ZAIRE

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    A systematic study of enteropathogenic agents in the stools of children was carried out in a rural area of Kivu Province in Zaire in June, 1979. 84 inpatients and 271 outpatients with diarrhoea were investigated together with 117 inpatient and 203 outpatient controls without diarrhoea. Campylobacter jejuni was the most frequently recovered pathogen in both inpatients (24%) and outpatients (13·7%). Enterotoxigenic Escherichia coliwas the next most common among children presenting as outpatients with diarrhoea (10·3%). Yersinia enterocoliticawas isolated for the first time in this region. Rotaviruses were found only in outpatients with diarrhoea, and usually in children under two years of age. C. jejuni and Vibrio choleraecould also be isolated from the faeces of domestic animals living in close contact with these village families. Analysis of the clinical features did not lead to the recognition of a pattern typical of any particular pathogen. Salmonella, Shigella, and enteropathogenic E. coli did not play a significant role in the cases studied. © 1983.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

    A competitive immunosorbent assay for the detection of heat-stable enterotoxin of Escherichia coli

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    A competitive ELISA procedure for the detection of Escherichia coli heat stable enterotoxin (ST) with monoclonal antibody has been developed. This test is 10 times more sensitive than the suckling-mouse bioassay and it is specific, simple and cheap. A set of 882 strains of E. coli isolated from man were tested both by ST-ELISA and suckling-mouse bioassay, the latter serving as the reference method. Positive results in both tests were obtained with 152 strains. The remaining strains gave negative results in both tests, with the exception of two strains, known to be ST producers, that gave negative results in the suckling-mouse assay, but gave positive results by the ELISA method.SCOPUS: ar.jinfo:eu-repo/semantics/publishe
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