Contents of tocols in different types of dry shell fruits

ArticleThe aim of this study was to identify all forms of vitamin E in different kinds of dry shell fruits (generally called ‘ nuts ’ ) obtained from different sources and to perform their mutual comparison . All tocols were determined by reversed phase HPLC using is ocratic elution with fluorescence detection. Almonds were evaluated as the most important source of α - tocopherol (average value 1 , 132 mg kg - 1 ), β - tocopherol was present in all samples as a minor component, its maximum content was found in hazelnuts (9.6 mg kg - 1 ). γ - T ocopherol was found in all kinds of nuts and the highest content was found in pistachios (584 mg kg - 1 ), very high amount of δ - tocopherol was contained in Brazil nuts (2 , 29 8 mg kg - 1 ). Tocotrienols were found in a smaller number of nut kinds than tocopherols. α - Tocotrienol was found only in three kinds of nuts - Brazil nuts, which contained the highest amount (399 mg kg - 1 ), pine nuts and hazelnuts grown in the Czech Republic, which were analyzed soon after harvest. Similarly, γ - tocotrienol w as det ermined only in four kinds of nuts (pistachios, macadamias, cashew and walnuts grown in the Czech Republic ) , which were analyzed soon after harvest ; t he most significant amount was found in pistachios (34.8 mg kg - 1 ) , β - and γ - tocotrienols were below the detection limit . In the Czech Republic, walnuts and hazelnuts are the most widely cultivated and consumed nuts. While in walnuts the most important form is γ - tocopherol (459 ± 40 mg kg - 1 ), in hazelnuts it is α - tocopherol ( 863 ± 10 mg kg - 1 )

A study of dynamics of bitter acids and xanthohumol in hop pellets during storage

ArticleEight varieties of hop pellets were analyzed for the contents of α - acids, ß - acids and xanthohumol according to the EBC 7.7 analytical method. The pellets were extracted with acidified mixture methanol - diethylether and analyzed using HPLC with a diode - array detector and a Nova - Pak column C 18 . Four series of analyses were performed: immediately after the unpacking of the pellets and then after five, seven and nine months of storage at 4 °C. According to the first ser i es of analyses, the contents were assayed as following (α - acids, ß - acids, xanthohumol resp., all in weight % in pellets): Galaxy (13.4, 8.0, 0.74), Citra (11.1, 3.0, 0.48), Tradition (8.2, 8.0, 0.58), Cascade (4.5, 5.2, 0.25), Northern Brewer (4.0, 2.9, 0. 37), Sládek (3.5, 4.0, 0.48), Saaz (2.0, 3.4, 0.24), and Triskel (1.7, 3.6, 0.18). According to these results, variety Galaxy was found as the richest in all three parameters. After nine months of storage at 4 °C, the weight loss of α - acids ranged from 4.1 % (Citra and Triskel) to 66.4% (Galaxy). The losses of β - acids and xanthohumol were less distinctive (from zero to 31.3% and 25.7%, resp.) and indicated good long storage possibilities of these compounds at convenient conditions (darkness, low temperature, elimination of direct influence of oxygen)

Content of flavan-3-ol monomers and gallic acid in grape seeds by variety and year

The content of flavan-3-ol monomers and gallic acid in grape seeds in the samples from the gene collection of Viticulture Research Station Karlštejn (Czech Republic) was investigated. Presence of catechin, epicatechin, epigallocatechin, epicatechin gallate and gallic acid was confirmed in these samples, other flavan-3-ol monomers like gallocatechin or epigallocatechin gallate were below limit of detection. As major flavan-3-ol monomers catechin and epicatechin with 85 % were detected. Average content of catechin in grape seed was 4454 ± 148 µg·g-1, 3085 ± 98 µg·g-1 epicatechin, 600 ± 41 µg·g-1 epigallocatechin, 457 ± 19 µg·g-1 gallic acid, and 352 ± 16 µg·g-1 epicatechin gallate. Variety had main impact on phenolic content followed by vintage. Average sum of flavan-3-ol monomers in grape seeds in white varietes was 7601 ± 273 µg·g-1 and 10869 ± 430 µg·g-1 in red varieties, with 10050 ± 425 µg·g-1 in 2012 and 7846 ± 219 µg·g-1 in 2013 were found on average in all varieties, respectively.  The highest phenolic content was characteristic for 'Pinot Noir', 'Muskat Donskoi', 'Aromriesling' and 'Hibernal' and may contribute to their health properties

Perfil bioquímico, inclusive proteinograma, do soro lácteo de búfalas primíparas e pluríparas sadias ao longo da lactação

Resumo: Para avaliar o perfil bioquímico, inclusive proteínas, do soro lácteo de búfalas Murrah primíparas e pluríparas sadias foram analisadas amostras de leite de 30 fêmeas bubalinas durante uma lactação completa. Os animais foram distribuídos em três grupos: G1 - 10 búfalas primíparas, G2 - 10 búfalas pluríparas com duas a três lactações e G3 - 10 búfalas pluríparas com mais de três lactações. O período de lactação foi dividido em: fase inicial (I: primeiro ao terceiro mês de lactação), fase intermediária (T: quarto ao sexto mês de lactação) e fase final (F: sétimo ao nono mês de lactação). Antes da colheita das amostras de leite foram realizados o exame físico da glândula mamária, o teste da caneca de fundo escuro e o California Mastitis Test (CMT). Após a assepsia dos quartos mamários, foram colhidas mensalmente, durante uma lactação completa, amostras de 20mL de leite de cada quarto mamário, em frascos plásticos esterilizados e sem conservante, para a realização do isolamento microbiológico, determinação do perfil bioquímico e fracionamento proteico por meio de eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE), e amostras de 30mL de leite de cada quarto mamário, em frascos plásticos esterilizados contendo conservante bronopol, para contagem de células somáticas (CCS). Das 1.042 amostras de leite colhidas dos três grupos experimentais durante a lactação, 923 amostras de leite apresentaram reação negativa ao CMT e isolamento microbiológico negativo e foram selecionadas para as análises do perfil bioquímico e fracionamento proteico em SDS-PAGE. Notou-se influência da ordem de parto e da fase da lactação no perfil bioquímico e no proteinograma do soro lácteo de búfalas da raça Murrah sadias. As búfalas primíparas (G1) apresentaram maior atividade das enzimas gamaglutamiltransferase (GGT: 2.346U/L) e fosfatase alcalina (ALP: 181U/L) e maiores concentrações de fósforo (P: 56,6mg/dL), potássio (K: 32,0mg/dL) e α-lactoalbumina (458mg/dL). As fêmeas com duas a três lactações (G2) apresentaram maior CCS (70.700 células/mL) e maiores concentrações de proteína total (1,55g/dL), albumina (100mg/dL), magnésio (Mg: 8,80mg/dL), cloretos (Cl: 176mg/dL), ferro (Fe: 10,7μg/dL), sódio (Na: 178mMol/L) e lactoferrina (59,5mg/dL). As fêmeas com mais de três lactações (G3) apresentaram maiores concentrações de cálcio total (Ca: 41,8mg/dL), cálcio ionizado (Cai: 2,92mMol/L), imunoglobulina A (IgA: 1,32mg/dL), albumina sérica (99,1mg/dL), imunoglobulina G (IgG: 49,7mg/dL) e b-lactoglobulina (1.068mg/dL). Durante a lactação foi observado aumento da CCS, aumento das atividades das enzimas GGT e ALP, aumento das concentrações de proteína total, albumina, P, Mg, Cl, Na, lactoferrina, albumina sérica, IgG, α-lactoalbumina e redução das concentrações de Ca, Fe, Cai, K, IgA e b-lactoglobulina no soro lácteo das búfalas. Os resultados obtidos podem ser utilizados como referências para a espécie bubalina e auxiliar no diagnóstico e no prognóstico de doenças de ocorrência comum na fase de lactação