Volatile Constituents of Leaves of Trifolium alexandrinum

This study presents the volatile constituents from leaves of Trifolium alexandrinum from Pakistan. The dried leaves were subjected to hydrodistillation for extraction and extracted volatile oil was subsequently characterized by gas chromatography-mass spectrometry technique. A total of 22 compounds were identified where the most dominant constituents were: phytol (46.00%), palmitic acid (9.26%), phytol acetate (6.44%), and linolenic acid (3.47%). Most of these are well-known constituents to exhibit antibacterial, antioxidant, antitumor, and anti-inflammatory activities