Effect of 28-homobrassinolide on the nitrate reductase, carbonic anhydrase activities and net photosynthetic rate in Vigna radiata

The foliage of fifteen day-old Vigna radiata seedlings was sprayed with water (control) or with a 10–10 M, 10–8Mor 10–6 Maqueous solution of 28-homobrassinolide (HBR). Samples of the treated plant material, collected at 30/50 days after sowing (DAS), were assessed for the activities of carbonic anhydrase (CA) and nitrate reductase (NR); leaf chlorophyll content (Chl); stomatal conductance (gs); carboxylation efficiency (CE), and net photosynthetic rate (PN). 28-homobrassinolide generated a significant impact on all these characteristics and on seed yield at harvest. Among the treatments, 10–8Mproved best, in which case the values for the above six physiological parameters in 30 days-old plants increased by, respectively, 31, 29, 27, 28, 29 and 33% over the control. Moreover, the harvest weight of the seeds of these plants was 27% larger than in the control

Simultaneous measurement of quality factor and wavelength shift by phase shift microcavity ring down spectroscopy

Optical resonant microcavities with ultra high quality factors are widely used for biosensing. Until now, the primary method of detection has been based upon tracking the resonant wavelength shift as a function of biodetection events. One of the sources of noise in all resonant-wavelength shift measurements is the noise due to intensity fluctuations of the laser source. An alternative approach is to track the change in the quality factor of the optical cavity by using phase shift cavity ring down spectroscopy, a technique which is insensitive to the intensity fluctuations of the laser source. Here, using biotinylated microtoroid resonant cavities, we show simultaneous measurement of the quality factor and the wavelength shift by using phase shift cavity ring down spectroscopy. These measurements were performed for disassociation phase of biotin-streptavidin reaction. We found that the disassociation curves are in good agreement with the previously published results. Hence, we demonstrate not only the application of phase shift cavity ring down spectroscopy to microcavities in the liquid phase but also simultaneous measurement of the quality factor and the wavelength shift for the microcavity biosensors in the application of kinetics measurements

Effect of heavy metal on survival of certain groups of indigenous soil microbial population

Heavy metal pollution of soil is known to adversely effect microbial activities at elevated concentration. However, response of indigenous soil bacterial population to added heavy metal and metal combinations is poorly understood. In the present study salts of heavy metals like Cu, Cd, Cr, Hg, Mn, Ni, Pb and Zn were added in soil under laboratory conditions with different concentrations (50, 100, 150 and 200 \u3bcg/g of soil) and sufficient moisture. The microcosm were stored at 28 \ub1 1 \ub0C for 28 days. Viable count of aerobic heterotrophs, asymbiotic nitrogen fixers and actinomycetes were determined at different time intervals (0, 7, 14, 21 and 28 days of incubation) using the plate dilution method. Aerobic- heterotrophic bacterial population were more sensitive to metal groups like Ni and Cd followed by Cu, Cd, Hg, Mn, Cr and Zn. Similarly a symbiotic nitrogen fixers showed higher sensitivity to metal groups like Cd, Pb, Hg followed by Cu, Cr, Mn, Ni and Zn. Actinomycetes were found most sensitive. Metal toxicity was higher for Pb, Mn, Ni followed by Cd, Hg, Cr and least to Cu and Zn. Toxicity of heavy metal was concentration as well as time dependent. Loss of microbial diversity is evident as we move towards higher concentration of heavy metal in soil. Further, experimentation is needed to understand the genetic diversity of the sensitive and metal tolerant microbial population and metal - microbe interaction under natural condition in soil. @JASE

Pelatihan Percakapan Bahasa Inggris Sehari-hari Bagi Kelompok Belajar Taman Berkah

Kemampuan berbicara Bahasa Inggris menjadi salah satu bagian yang sangat penting dalam menghadapi perkembangan zaman. Pelatihan ini bertujuan untuk melatih dan memperkenalkan Bahasa Inggris dalam konteks percakapan sehari-hari. Peserta yang terlibat dalam kegiatan pelatihan ini berjumlah 10 peserta. Pelatihan dilaksakan pada tanggal 4-6 September 2023 yang bertempat di Kelompok Belajar Taman Berkah.Hasil dari pelatihan ini sebagian besar peserta telah menunjukkan kemampuan dan perubahannya dalam berbicara Bahasa inggris dengan menggunakan metode Daily Conversation dapat dilihat dari perolehan skor pada post-tes yang dilakukan. Rata-rata peserta pelatihan memperoleh level 3 yaitu mencakup situasi percakapan yang lebih umum, dapat mengambil inisiatif dalam percakapan sehari-hari, kecepatan berbicara sedikit lambat namun tidak terlihat jeda, pelafalan terlihat cukup jelas.

Model Education Reconstruction (Mer) Bahan Ajar Penelitian Laboratorium (Pl) Konteks Zeolit Berbasis Problem Solving-decision Making (Psdm)

. Aspects discussed in this research is to improve the intelligence thinking of student research through a MER (Duit 2012). Based on a problem that is often found that the appearance of lack of originality of research because students are not able to think of research, which is shown in a preliminary study.Based on these problems, researchers developed teaching materials to reconstruct RL context zeolite based PSDM. The instrument was a questionnaire response experts to determine the feasibility of the cognitive level of the student materials in accordance with the character of the curriculum in 2013. The characteristics of instructional materials: 1) was developed in accordanceaspect PSDM attitude Myers-Briggs Type Indicator (MBTI), 2) the context ofthe lecture corresponding zeolite mineral chemistry issues, 3) content according to the level of cognitive student accesible criteria, 4) Perspective students to the concept of material through interviews. Interviews showed> 80% of the students have any preconceptions about the meaning of the scientific method and thinking research. Therefore, the insertion of material thought PSDM based research on teaching materials need to be raised. Validation / CVR obtain the Value 0.76, and CVI c(0,76) > CVI t(0,68) showed very appropriate instructional materials used

Irreversibilities in a triple diffusive flow in various porous cavities

Entropy generation minimization approach is a very good method allowing to analyze the engineering systems to exclude technical failure. The present study deals with computational analysis of triple diffusive flow, energy transference and entropy production in different porous cavities from square to triangular through trapezoidal shape. The formulated boundary-value problem has been worked out using the finite element technique and non-primitive variables. The developed computational code has been verified using numerical results of other researchers. Analysis of entropy production due to energy and mass transport, motion friction, and porous material has been performed for different chamber's shapes. Entropy generation analysis in chambers of various geometries under the triple-diffusive flow is a novelty of the present research, where different entropy production mechanisms have been scrutinized for one complex problem. It has been ascertained that average total entropy generation strength raises with buoyancy ratios, Lewis and Rayleigh numbers, but it has the minimum value for the square chamber in comparison with triangular and trapezoidal shapes. Moreover, obtained results characterize a neglecting influence of motion friction on the total entropy generation

Investigation of the cardiac depressant effect of Caralluma tuberculate N.E.Br on isolated rabbit heart

Purpose: To investigate the histopathological and cardiac depressant effect of the aqueous methanol extract of Caralluma tuberculata N.E. Br (AMECT) (family: Asclepiadaceae)’ and to determine if there is a scientific basis for its cardiovascular diseases-related folkloric use. Methods: The effect of AMECT in different concentrations ranging from 0.00001 to 1.0 mg/mL were evaluated in isolated perfused rabbit heart to assess their effect on the force of contraction and heart rate using Langendorff’s apparatus. Atropine and adrenaline were used to identify the underlying mechanism of response produced by AMECT. The extract was studied for its possible mechanism in the absence and presence of atropine and adrenaline. In addition, sub-chronic toxicity and histopathological study of heart tissues in rats were assessed by administering 500 mg/kg of extract. Results: At all concentrations, AMECT produced significant (p < 0.001) negative ionotropic and negative chronotropic effects. The most significant effect was observed at 0.001 mg/mL and higher concentrations hence 0.001 mg/mL was selected for further studies. Pre-incubation with atropine did not significantly inhibit the effects of AMECT. However, AMECT significantly (p < 0.01) blocked the cardiac stimulant effect of adrenaline. In the histopathological studies, AMECT did not produce any significant cellular changes or signs of toxicity in the sub-chronic toxicity study. Conclusion: The cardiac-depressant responses of AMECT may involve the β-adrenergic receptors in the myocardium of isolated rabbit heart thus confirming the rationale for its use in ethnomedicine for cardiac diseases

Exploring the potential of Red Kidney Bean (Phaseolus vulgaris) to develop protein Based Product for food Applications.

ABSTRACT Protein isolate was prepared from red kidney beans and its functional properties were evaluated at different pH levels to access its suitability for food applications. Carbohydrates, crude protein, crude fiber, crude fat and ash contents of red kidney bean seeds were found to be 53.02±1.14%, 25.78±0.77%, 6.82±0.31%, 1.92±0.15% and 4.34±0.20%, respectively. Magnesium, calcium, sodium, potassium and iron were observed as macro elements in red kidney bean seeds. Protein solubility, emulsification, gelation as well as foaming properties of the bean protein isolate were significantly (P≤0.05) affected by different pH levels. The solubility, emulsifying activity and stability as well as foam capacity of the protein isolate were dependant with minimal values observed at pH 4 while maximum at pH 10. Contrarily, the stability of foam was highest at pH 4 while a decreasing trend in foam stability was observed with increase of pH. Gelation properties improved at acidic pH with maximum gelation capacity observed at pH 4 while these properties decreased at alkaline conditions. Conclusively, red kidney beans can be utilized to prepare protein isolate whose functional properties can be modified by changing the pH of the environment for better utilization in the food formulation systems