Investigation of Toxicological Effects of di-2-ethylhexyl with Fish Embryo Test (FET) in Zebrafish (Danio rerio)

Di(2-ethylhexyl)phthalate (DEHP) is a plasticizer in a variety of consumer products. Given the adverse toxicity effects of DEHP on living organisms, its high consumption and dispersal has been caused a serious concern. . Nowedays, the Fish Embryo Test (FET) method is widely used among the conventional methods of toxicology .The aim of the this study was to determine the acute toxicity of Di(2-ethylhexyl)phthalate on zebrafish (Danio rerio) embryo using FET method to assess both lethal and sublethal endpoints and anomalies effects over 5 days . Zebrafish embryos (3 hours post-fertilization) were exposed to different concentrations of DEHP (0.05, 5, 20, 35, 50 and 100 µg/l) for 120 h, then, lethal and sub-letahl endpoints of embryos evaluated and data were analized using Probit software. The LC50, NOEC and LOEC values obtained for DEHP were 46.14, 12.08 and 22.06 µg/l, respectively. Also, There were abnormalities including  coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac, malformation of tail and lack of heartbeat in the embryos. This study showed that DEHP is a teratogenic pollutants and the application of FET method is / an alternative method for developmental toxicity tests to predict effects of emerging pollutants on aquatic organisms

Assessment of mercury concentration in pike (Esox Lucius) and the evaluation of potential human health risks due to its consumption, Anzali wetland

The purpose of this study was to evaluate the mercury concentration of pike muscle and its potential consumption risk. Daily, weekly, and monthly maximum allowable consumption were calculated for children and adults. The average mercury concentration was lower than National Iranian Standards Organization and international organizations. The HQ index results indicated no potential threat to consumers. In addition, the absence of health risk for the species itself was confirmed. However, due to the carnivorous feeding habit of this species and the bio-accumulation of mercury, continuous monitoring should be carried out to ensure the health of the species and humans