Pre-flight optical test and calibration for the Cosmic Infrared Background ExpeRiment 2 (CIBER-2)

The total integrated emission from galaxies, known as the Extragalactic Background Light (EBL), is an important observable for understanding the history of star formation over the history of the universe. Spatial fluctuations in the infrared EBL as measured by the Cosmic Infrared Background ExpeRiment (CIBER), Spitzer and AKARI exceed the predicted signal from galaxy clustering alone. The CIBER-2 project seeks to extend CIBER observa- tions of the EBL throughout the near infrared into the optical, through measurements above Earth's atmosphere during a suborbital sounding rocket flight. The experiment has a LN2-cooled 28.5 cm Cassegrain telescope along with three optical paths and dichroic beamsplitters, which are used to obtain three wide-field images in six broad spectral bands between 0.5-2.0 μm. The three focal planes also contain linear variable filters (LVFs) which simultaneously take spectra with resolution R=20 across the same range. CIBER-2 is scheduled to y multiple times on a Black Brant IX sounding rocket from White Sands Missile Range in the New Mexico desert. For the first flight, scheduled for early 2021, we have completed a variety of pre-flight optical tests, which we use to make focus adjustments, spectral response measurements, and absolute photometric calibrations. In this paper, we describe the methods behind these tests and present their results for pre-flight performance evaluation. In particular, we present measurements of the PSF for each broad spectral band, along with absolute calibration factors for each band and the LVF. Through monochromator scans, we also measure the spectral responsivity of each LVF as a function of position

A case of pulmonary adenocarcinoma showing rapid progression of peritoneal dissemination after immune checkpoint inhibitor therapy

Abstract Background Immune checkpoint inhibitors are standard treatments for non-small cell lung cancer. Unique cases with paradoxical acceleration of the disease after immunotherapy have been reported. These have been described as cases of hyperprogressive disease. Case presentation A 76-year-old man was diagnosed with pulmonary adenocarcinoma with pleural dissemination and liver and adrenal metastases. Genomic analysis revealed neither EGFR mutations nor ALK translocations. Immunohistochemical analysis revealed a programmed death-ligand 1 tumor proportion score of 23%. Chemotherapy with carboplatin, paclitaxel, and bevacizumab resulted in Grade 3 skin eruption and disease progression. Pembrolizumab was initiated as a second-line treatment. However, peritoneal dissemination and ascites developed. The patient died 2 weeks later. The autopsy revealed widespread peritoneal dissemination and an extensive hemorrhagic infarction. Conclusion This was a rare case of hyperprogressive disease with rapid progression of peritoneal dissemination after pembrolizumab treatment

Steady-state and dynamic gene expression programs in Saccharomyces cerevisiae in response to variation in environmental nitrogen

Cell growth rate is regulated in response to the abundance and molecular form of essential nutrients. In Saccharomyces cerevisiae (budding yeast), the molecular form of environmental nitrogen is a major determinant of cell growth rate, supporting growth rates that vary at least threefold. Transcriptional control of nitrogen use is mediated in large part by nitrogen catabolite repression (NCR), which results in the repression of specific transcripts in the presence of a preferred nitrogen source that supports a fast growth rate, such as glutamine, that are otherwise expressed in the presence of a nonpreferred nitrogen source, such as proline, which supports a slower growth rate. Differential expression of the NCR regulon and additional nitrogen-responsive genes results in >500 transcripts that are differentially expressed in cells growing in the presence of different nitrogen sources in batch cultures. Here we find that in growth rate–controlled cultures using nitrogen-limited chemostats, gene expression programs are strikingly similar regardless of nitrogen source. NCR expression is derepressed in all nitrogen-limiting chemostat conditions regardless of nitrogen source, and in these conditions, only 34 transcripts exhibit nitrogen source–specific differential gene expression. Addition of either the preferred nitrogen source, glutamine, or the nonpreferred nitrogen source, proline, to cells growing in nitrogen-limited chemostats results in rapid, dose-dependent repression of the NCR regulon. Using a novel means of computational normalization to compare global gene expression programs in steady-state and dynamic conditions, we find evidence that the addition of nitrogen to nitrogen-limited cells results in the transient overproduction of transcripts required for protein translation. Simultaneously, we find that that accelerated mRNA degradation underlies the rapid clearing of a subset of transcripts, which is most pronounced for the highly expressed NCR-regulated permease genes GAP1, MEP2, DAL5, PUT4, and DIP5. Our results reveal novel aspects of nitrogen-regulated gene expression and highlight the need for a quantitative approach to study how the cell coordinates protein translation and nitrogen assimilation to optimize cell growth in different environments

Carotid artery intima-media thickness, HDL cholesterol levels, and gender associated with poor visual acuity in patients with branch retinal artery occlusion.

PurposeTo investigate factors associated with poor visual acuity (VA) in branch retinal artery occlusion (BRAO).MethodsThis was a retrospective cross-sectional study of 72 eyes with BRAO of 72 patients. For statistical comparison, we divided the patients into worse-VA (decimal VA = 0.5) groups. We examined the association of clinical findings, including blood biochemical test data and carotid artery ultrasound parameters, with poor VA.ResultsMedian age, hematocrit, hemoglobin and high-density lipoprotein (HDL) differed significantly between the groups (P = 0.018, P ConclusionsWe found that increased IMT-Bmax, decreased HDL, and female sex were associated with poor VA in BRAO patients. Our findings might suggest novel risk factors for visual dysfunction in BRAO and may provide new insights into the pathomechanisms underlying BRAO