Assessing Drought Tolerance of Newly Developed Tissue-Cultured Canola Genotypes under Varying Irrigation Regimes

This article belongs to the Special Issue Crop Tolerance under Biotic and Abiotic Stresses[Abstract] Drought is a major abiotic stress that greatly affects canola growth, production, and quality. Moreover, water scarcity is projected to be more severe and frequent as a result of climate change, in particular in arid environments. Thereupon, developing drought-tolerant and high-yielding canola genotypes has become more critical to sustaining its production and ensuring global food security with the continuing population growth. In the present study, ten canola genotypes comprising six developed tissue-cultured canola genotypes, two exotic genotypes, and two commercial cultivars were evaluated under four irrigation regimes. The applied irrigation regimes were well-watered (100% crop evapotranspiration, ETc), mild drought (80% ETc), moderate drought (60% ETc), and severe drought (40% ETc) conditions. Drought-stress treatments (80, 60, and 40% ETc) gradually reduced the chlorophyll content, relative water content, flowering time, days to maturity, plant height, number of pods, number of branches, seed yield, and oil percentage, and increased proline, phenolic, anthocyanin, and glycine betaine contents. The evaluated genotypes exhibited varied responses to drought-stress conditions. The developed tissue-cultured genotypes T2, T3, and T1, as well as exotic genotype Torpe, possessed the highest performance in all evaluated parameters and surpassed the other tested genotypes under water-deficit conditions. Overall, our findings elicited the superiority of certain newly developed tissue-cultured genotypes and exotic ones compared with commercial cultivars, which could be exploited in canola breeding under water-deficit conditions.This research was funded by the Researchers Supporting Project number (RSPD-2023R730), King Saud University, Riyadh, Saudi ArabiaKing Saud University (Riad, Arabia Saudí); RSPD-2023R73

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Seed Priming and Foliar Application with Ascorbic Acid and Salicylic Acid Mitigate Salt Stress in Wheat

Ascorbic acid (AA) and salicylic acid (SA) are naturally active antioxidants that protect against plant stresses, including salinity. We studied the physiological response of wheat to AA and SA (100, 200 ppm) as well as the combined treatment of AA and SA (100 ppm) through application as both priming and foliar spray treatments under saline conditions. The results showed that wheat plants under salt-affected soils exhibited numerous physiological effects in plant metabolism, which subsequently affected the qualitative and quantitative parameters of growth and yield. Moreover, the photosynthetic pigments, antioxidant content, and yield are significantly enhanced under the combined treatment of AA and SA. In contrast, the application of AA and SA lowered the osmolytes and lipid peroxidation content under saline conditions. Accordingly, the enhancement of the mentioned parameter was related to the scavenging of the reactive oxygen species and decreasing the oxidative stress on the plant under the salinity stress. Our results explore the significance of applied AA and SA as efficacious compounds in wheat farming under saline conditions. The combined application of (100 ppm) AA with (100 ppm) SA using priming or a foliar spray can be a promising treatment for beneficent wheat growth and productivity improvement under salt-affected soil conditions

Seed Priming and Foliar Application with Ascorbic Acid and Salicylic Acid Mitigate Salt Stress in Wheat

Ascorbic acid (AA) and salicylic acid (SA) are naturally active antioxidants that protect against plant stresses, including salinity. We studied the physiological response of wheat to AA and SA (100, 200 ppm) as well as the combined treatment of AA and SA (100 ppm) through application as both priming and foliar spray treatments under saline conditions. The results showed that wheat plants under salt-affected soils exhibited numerous physiological effects in plant metabolism, which subsequently affected the qualitative and quantitative parameters of growth and yield. Moreover, the photosynthetic pigments, antioxidant content, and yield are significantly enhanced under the combined treatment of AA and SA. In contrast, the application of AA and SA lowered the osmolytes and lipid peroxidation content under saline conditions. Accordingly, the enhancement of the mentioned parameter was related to the scavenging of the reactive oxygen species and decreasing the oxidative stress on the plant under the salinity stress. Our results explore the significance of applied AA and SA as efficacious compounds in wheat farming under saline conditions. The combined application of (100 ppm) AA with (100 ppm) SA using priming or a foliar spray can be a promising treatment for beneficent wheat growth and productivity improvement under salt-affected soil conditions