48 research outputs found
Quinacrine, A Chromosome Stain Specific for Deoxyadenylate-Deoxythymidylate-Rich Regions in DNA
PROTEIN-DNA RECOGNITION: THE INTERACTION OF Jac, cI AND Z. coli RNA POLYMERASE WITH OPERATORS AND PROMOTERS
Current methods for synthesizing DNA utilize protected deoxynucleoside
phosphoramidites as synthons and a glass or silica gel solid support as the
synthesis matrix. Syntheses are extremely rapid (5-10 minutes per cycle) and lead
to high yields of deoxyoligonucleotides containing 20-100 mononucleotides each.
Using these methods to synthesize modified DNAs, the interaction of Z. coli RNA
polymerase and cI repressor with lambda Pp promoter has been studied. Results
identify certain 5-methyl groups located in the -35 region of the promoter and Opl
that are important protein recognition sites. These results correlate quite nicely
to previous research with Jac repressor. Additional data suggest possible
insights regarding the formation of open promoter complexes