14 research outputs found
Identification of viruses infecting cucurbits and determination of genetic diversity of cucumber mosaic virus in Lorestan province, Iran
Various viral pathogens infect Cucurbitaceae and cause economic losses. Th e aim of the
present study was to detect plant viral pathogens including Cucumber mosaic virus (CMV),
Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Cucurbit
yellow stunting disorder virus (CYSDV) and Cucurbit chlorotic yellows virus (CCYV)
in Lorestan province, in western Iran, and also to determine CMV genetic diversity in Iranian
populations. A total of 569 symptomatic leaf samples were collected in 2013 and 2014
from cucurbits growing regions in Lorestan province. Th e collected samples were assessed
for viral diseases by ELISA. Th e results showed virus incidences in most regions. Th en,
the infection of 40 samples to CMV was confi rmed by RT-PCR. Moreover, to distinguish
between the two groups (I and II) of CMV, PCR products were digested by two restriction
enzymes XhoI and EcoRI. Results of the digestion showed that the isolates of Lorestan
belonged to group I. Th e CMV-coat protein gene of eight isolates from diff erent regions
and hosts was sequenced and phylogenetic analysis was performed. Subsequent analyses
showed even more genetic variation among Lorestan isolates. Th e phylogenetic tree revealed
that Lorestan province isolates belonged to two IA and IB subgroups and could be
classifi ed together with East Azerbaijan province isolates. Th e results of the present study
indicate a wide distribution of CMV, ZYMV, CGMMV, CYSDV and CCYV viruses in cucurbits
fi elds of Lorestan province and for the fi rst time subgroup IB of CMV was reported
on melon from Iran