Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light

Purpose To determine the effects of visible light on development of mouse embryos and the potential of fibroblast cells to overcome deleterious effects of visible light on mouse preimplantation stage embryos. Methods Two-cell mouse embryos were randomly allocated to un-exposed group (control) and exposed group receiving 1600 lx visible light for various time lengths. Both exposed and un-exposed embryos were co-cultured with either Mouse Embryonic Fibroblast (MEF) or Human Embryonic Fibroblast (HEF). Developmental rate of embryos at day 3 (morula), 4 (expanded blastocyst) and 5 (hatching or hatched blastocyst) was evaluated. Results Exposure of embryos to visible light for 30 min decreased developmental rate significantly (P<0.01). Developmental rate of exposed embryos co-cultured with MEF (58%; p<0.05 both at day 4 and 5) and HEF (67%; P<0.01 both at day 4 and 5) was higher than control. Conclusions Visible light adversely affects embryo development in a time-dependent manner. Feeder cells may enhance embryo development particularly when suboptimal conditions are involved

IMPLEMENTATION OF SPEECH RECOGNITION HOME CONTROL SYSTEM USING ARDUINO

ABSTRACT Electronically control of household activities has long been explored in various ways. From electronic remote control using infra-red sensors, to voice-controlled application, we are continuously trying to find a better way to control electrical and electronic devices to ease our daily life. This paper presents the development of a low cost remote home control system using speech recognition. The system focused on controlling fan and lamp wirelessly by applying speech recognition into the system, with Arduino Uno as the controller. Two different units were developed: the main control unit which also act as the transmitter and the receiver unit which controls the fan and lamp. The main control unit accept voice command from the user and convert it into text by using Easy VR shield. The signal is then transmitted to the receiver using RF signal. With the use of RF technology, the system is able to work wirelessly. This system is hoped to be able to help people to use the electronics devices effectively besides provide improved convenience and comfort to user especially for elderly and disabled who live alone and help them to be more independent