37 research outputs found
Recent Advances in Fluorescent Labeling Techniques for Fluorescence Microscopy
Tremendous progress in recent computer-controlled systems for fluorescence and laser-confocal microscopy has provided us with powerful tools to visualize and analyze molecular events in the cells. Various fluorescent staining and labeling techniques have also been developed to be used with these powerful instruments. Fluorescent proteins such as green fluorescent protein (GFP) allow us to directly label particular proteins of interest in living cells. This technique has been extended over a large area of cell biology, and a variety of fluorescent protein-derived techniques have been developed to visualize the functions and conditions of the molecules within living cells. In this review, we summarize the techniques for fluorescent staining and labeling for recent fluorescence microscopy
H-ZSM-5ゼオライト上でのオレフィンの水素化
H-ZSM-5ゼオライトのオレフィン水素化能を調べるため, 1段目の反応管にZn-Cr系メタノール合成触媒, 2段目の反応管にH-ZSM-5 ゼオライトを充てんした2段反応装置を用いて, メタノールを経由する合成ガスからの炭化水素合成を検討した。1段目の反応圧を40kg/cm2とし, 2段目の反応圧を5~45kg/cm2と変化させたときの結果をFig. 1に示す。2段目の反応圧が20kg/cm2以上では, エタン, プロパンは生成したが, エチレン, プロピレンは全く生成しなかった。このことは, これらパラフィンがH-ZSM-5ゼオライト上で水素化あるいは水素移行反応により生成したことを示唆している。この違いを明らかにするためC2H4-H2, C2H4-Heの2種類の混合ガスをH-ZSM-5ゼオライトに導入した (Fig. 2)。C2H4-H2系では, エタン収率は反応温度と共に著しく増大し, 545°Cでは導入したエチレンの95%がエタンとして検出された。一方, 水素移行反応によるパラフィン生成の可能性を調べるため行ったC2H4-He系では, 550°Cで導入したエチレンの10%がエタンとして検出された。したがって, C2H4-H2系で生成したエタンの大部分はエチレンの水素化により生成したことになる。
また, Fig. 3には, 不純物の影響を調べるため, Feを導入したZSM-5型ゼオライトを用いて合成ガスの転化反応を行った結果を示す。Fe(II) より調製したZSM-5型ゼオライトの生成物はオレフィン指向であった。Fe(II) とAlより調製し, プロトン化した酸点をもつH-ZSM-5型ゼオライトの生成物はパラフィン指向であった。
以上の結果より, H-ZSM-5ゼオライトはオレフィン水素化能を有していると結論した。Conversion of synthesis gas to hydrocarbons was carried out in a two-stage system consisting of a methanol synthesis reactor followed by a hydrocarbon-forming reactor containg H-ZSM-5 zeolite. It was found that the H-ZSM-5 zeolite catalyst was active in olefin hydrogenation
Ultrastructural observation on 'transitional tubules' in human oviductal ciliogenic cells
Design and Numerical Analysis of Vibration Isolators With Quasi-Zero-Stiffness Characteristics Using Bistable Foldable Structures
Immunocytochemistry of the striated rootlets associated with solitary cilia in human oviductal secretory cells
Ultrastructural and immunohistochemical study of the basal apparatus of solitary cilia in the human oviduct epithelium
The basal apparatus of the solitary cilium is composed of the basal body, an associated centriole and the basal body-associated structures. To see the connection between the basal body and the centriole, we studied the basal apparatus of solitary cilia in human oviductal secretory cells by electron microscopy and immunohistochemistry. A single centriole was present in the vicinity of the basal body of a solitary cilium. The basal body and the single centriole were interconnected by one or two bundles of thin filaments with a few periodic striations. We have called these bundles the striated connector. The periodicity of striations in the striated connector measured 55 ± 6 nm, about 15 nm shorter than that of striated rootlets. The striated connector was immunolabelled with R67 antibody specific to striated rootlets, indicating that they are composed of common molecule(s). Although the true function of the connector is unknown as yet, it could play an important role for stabilising the basal body in the apical cytoplasm