The surface chemistry of diamond

Imperial Users onl

Stochastic compartmental analysis - Some applications and examples of estimation in a pulse labelled system

Stochastic compartmental analysis with examples of estimation in pulse labelled syste

A cell-permeable biscyclooctyne as a novel probe for the identification of protein sulfenic acids

Reactive oxygen species act as important second messengers in cell signaling and homeostasis through the oxidation of protein thiols. However, the dynamic nature of protein oxidation and the lack of sensitivity of existing molecular probes have hindered our understanding of such reactions; therefore, new tools are required to address these challenges. We designed a bifunctional variant of the strained bicyclo[6.1.0]nonyne (BCN-E-BCN) that enables the tagging of intracellular protein sulfenic acids for biorthogonal copper-free click chemistry. In validation studies, BCN-E-BCN binds the sulfenylated form of the actin-severing protein cofilin, while mutation of the cognate cysteine residues abrogates its binding. BCN-E-BCN is cell permeable and reacts rapidly with cysteine sulfenic acids in cultured cells. Using different azide-tagged conjugates, we demonstrate that BCN-E-BCN can be used in various applications for the detection of sulfenylated proteins. Remarkably, cycloaddition of an azide-tagged fluorophore to BCN-E-BCN labelled proteins produced in vivo can be visualized by fluorescence microscopy to reveal their subcellular localization. These findings demonstrate a novel and multifaceted approach to the detection and trapping of sulfenic acids

On the hydrographic mechanism of the so-called brown zones associated with tidal glaciers

The presence of zones of muddy, ice-free water at the sea faces of tidal glaciers has been recorded from Alaska (Gilbert, 1910), and from Spitzbergen (Stott, 1936). In view of their great significance in local ecology an understanding of the causes of these so-called Brown Zones seemed desirable and the problem of their establishment was first attacked hydrographically by the Oxford University Expedition to Spitzbergen, in 1933...

A Dream : Waltz

https://digitalcommons.library.umaine.edu/mmb-ps/2509/thumbnail.jp

Perspectives on the Missiological Legacy of Martin Luther and the Protestant Reformation

Upon the occasion of the 500th anniversary Martin Luther’s publication of his 95 theses, this composite article brings together five perspectives on the missiological legacy of the reformer and the subsequent Protestant Reformation. The blend of voices makes clear that Luther and the subsequent Protestant Reformation do not have a simple missiological legacy but rather various legacies: theological, ecclesiological, political, and practical; some of which co-exist, and even collide, in the same ecclesiastical community. The scandalous legacy of a splintered and splintering church remains. Yet, demonstrations of mutual recognition, reciprocal respect, and genuine fellowship can be found in certain missiological circles

Hemiparasitic plant impacts animal and plant communities across four trophic levels

1.Understanding the impact of species on community structure is a fundamental question in ecology. There is a growing body of evidence that suggests that both sub-dominant species and parasites can have a disproportionately large impact. 2.Here we report the impacts of an organism that is both subdominant and parasitic, the hemiparasite Rhinanthus minor. Whilst the impact of parasitic angiosperms on their hosts and, to a lesser degree, co-existing plant species, have been well characterized, much less is known about their impacts on higher trophic levels. 3.We experimentally manipulated field densities of the hemiparasite Rhinanthus minor in a species rich grassland, comparing the plant and invertebrate communities in plots where it was removed, at natural densities or at enhanced densities. 4.Plots with natural and enhanced densities of R. minor had lower plant biomass than plots without the hemiparasite, but enhanced densities almost doubled the abundance of invertebrates within the plots across all trophic levels, with effects evident in herbivores, predators and detritivores. 5.The hemiparasite R. minor, despite being a sub-dominant and transient component within plant communities that it inhabits, has profound effects on four different trophic levels. These effects persist beyond the life of the hemiparasite, emphasizing its role as a keystone species in grassland communitie

The Indian family on UK reality television: Convivial culture in salient contexts

This is the author's accepted manuscript. The final published article is available from the link below, copyright 2012 @ the author.This article demonstrates how The Family (2009), a fly-on-the wall UK reality series about a British Indian family, facilitates both current public service broadcasting requirements and mass audience appeal. From a critical cultural studies perspective, the author examines the journalistic and viewer responses to the series where authenticity, universality, and comedy emerge as major themes. Textual analysis of the racialized screen representations also helps locate the series within the contexts of contested multiculturalism, genre developments in reality television and public service broadcasting. Paul Gilroy’s concept of convivial culture is used as a frame in understanding how meanings of the series are produced within a South Asian popular representational space. The author suggests that the social comedy taxonomy is a prerequisite for the making of this particular observational documentary. Further, the popular (comedic) mode of conviviality on which the series depends is both expedient and necessary within the various sociopolitical contexts outlined