Standard methods for Apis mellifera anatomy and dissection

An understanding of the anatomy and functions of internal and external structures is fundamental to many studies on the honey bee Apis mellifera. Similarly, proficiency in dissection techniques is vital for many more complex procedures. In this paper, which is a prelude to the other papers of the COLOSS BEEBOOK, we outline basic honey bee anatomy and basic dissection techniques

Bio-Futures for Transplanetary Habitats: A Summary and Key Outcomes from the 2022 Symposium

Bio-Futures for Transplanetary Habitats (BFfTH) is a Special Interest Group within the Hub for Biotechnology in the Built Environment that aims to explore and enable interdisciplinary research on transplanetary habitats and habitats within extreme environments through an emphasis on the bio-social and biotechnological relations. BFfTH organized the online and onsite networking symposium Bio-Futures for Transplanetary Habitats to examine how emerging biotechnologies, living materials, and more-than-human life can be implemented in habitat design and mission planning. The two day symposium aimed to serve as a catalyst in establishing an international network of collaborators across industry, academia and the private sector. It also aimed to support the development of novel methodologies to move beyond discipline-specific approaches in order to address and interrogate emerging questions surrounding potential transplanetary habitats and habitats in extreme environments. The symposium was divided into five sessions which hosted a minimum of three speakers each, these sessions were: Mycelium for Mars, Plants and Agriculture, Sustainable Habitats and Travels, Artistic Approach to Extremes Habitats, and Novel Biotechnologies for Space Habitats. This paper presents key outcomes from the symposium sessions, moderated panel, and informal discussions. The trends in ongoing research are identified and summarized following the use of biotechnology and bio-design to ensure and support safety, sustainability, habitability, reliability, crew efficiency, productivity and comfort in extreme environments both here on Earth and off-world. Moving beyond pure design and engineering innovation, the outcomes of this symposium also further interrogates sociotechnical imaginaries. Biodesign-based and biotechnologically-enabled transplanetary futures are investigated to understand how we want these futures to behave, feel and be experienced. The symposium hosted a wide range of topics including: innovative material-driven processes for the design of transplanetary habitats; socio-political concerns or ethical implications to be taken into account; technology transfer and transitioning towards a sustainable built environment on Earth; multi-species narratives and relations to sustain human and other-than-human life in transplanetary habitats; sociotechnical considerations in propagating and sustaining Earthbound life beyond Earth environments; and sustainable living on Earth through a holistic systems thinking approach. BFfTH further reflects on what potential bio-social and biotechnological research is needed to sustain life in an extraterrestrial environment in the future and how it can help with transitioning towards a more sustainable built environment here on Earth in the present

Bio-Futures for Transplanetary Habitats: A Summary and Key Outcomes from the 2022 Symposium

Bio-Futures for Transplanetary Habitats (BFfTH) is a Special Interest Group within the Hub for Biotechnology in the Built Environment that aims to explore and enable interdisciplinary research on transplanetary habitats and habitats within extreme environments through an emphasis on the bio-social and biotechnological relations. BFfTH organized the online and onsite networking symposium Bio-Futures for Transplanetary Habitats to examine how emerging biotechnologies, living materials, and more-than-human life can be implemented in habitat design and mission planning. The two day symposium aimed to serve as a catalyst in establishing an international network of collaborators across industry, academia and the private sector. It also aimed to support the development of novel methodologies to move beyond discipline-specific approaches in order to address and interrogate emerging questions surrounding potential transplanetary habitats and habitats in extreme environments. The symposium was divided into five sessions which hosted a minimum of three speakers each, these sessions were: Mycelium for Mars, Plants and Agriculture, Sustainable Habitats and Travels, Artistic Approach to Extremes Habitats, and Novel Biotechnologies for Space Habitats. This paper presents key outcomes from the symposium sessions, moderated panel, and informal discussions. The trends in ongoing research are identified and summarized following the use of biotechnology and bio-design to ensure and support safety, sustainability, habitability, reliability, crew efficiency, productivity and comfort in extreme environments both here on Earth and off-world. Moving beyond pure design and engineering innovation, the outcomes of this symposium also further interrogates sociotechnical imaginaries. Biodesign-based and biotechnologically-enabled transplanetary futures are investigated to understand how we want these futures to behave, feel and be experienced. The symposium hosted a wide range of topics including: innovative material-driven processes for the design of transplanetary habitats; socio-political concerns or ethical implications to be taken into account; technology transfer and transitioning towards a sustainable built environment on Earth; multi-species narratives and relations to sustain human and other-than-human life in transplanetary habitats; sociotechnical considerations in propagating and sustaining Earthbound life beyond Earth environments; and sustainable living on Earth through a holistic systems thinking approach. BFfTH further reflects on what potential bio-social and biotechnological research is needed to sustain life in an extraterrestrial environment in the future and how it can help with transitioning towards a more sustainable built environment here on Earth in the present.System Engineerin

Observation Versus Intervention for Low-Grade Intracranial Dural Arteriovenous Fistulas

Abstract BACKGROUND Low-grade intracranial dural arteriovenous fistulas (dAVF) have a benign natural history in the majority of cases. The benefit from treatment of these lesions is controversial. OBJECTIVE To compare the outcomes of observation versus intervention for low-grade dAVFs. METHODS We retrospectively reviewed dAVF patients from institutions participating in the CONsortium for Dural arteriovenous fistula Outcomes Research (CONDOR). Patients with low-grade (Borden type I) dAVFs were included and categorized into intervention or observation cohorts. The intervention and observation cohorts were matched in a 1:1 ratio using propensity scores. Primary outcome was modified Rankin Scale (mRS) at final follow-up. Secondary outcomes were excellent (mRS 0-1) and good (mRS 0-2) outcomes, symptomatic improvement, mortality, and obliteration at final follow-up. RESULTS The intervention and observation cohorts comprised 230 and 125 patients, respectively. We found no differences in primary or secondary outcomes between the 2 unmatched cohorts at last follow-up (mean duration 36 mo), except obliteration rate was higher in the intervention cohort (78.5% vs 24.1%, P < .001). The matched intervention and observation cohorts each comprised 78 patients. We also found no differences in primary or secondary outcomes between the matched cohorts except obliteration was also more likely in the matched intervention cohort (P < .001). Procedural complication rates in the unmatched and matched intervention cohorts were 15.4% and 19.2%, respectively. CONCLUSION Intervention for low-grade intracranial dAVFs achieves superior obliteration rates compared to conservative management, but it fails to improve neurological or functional outcomes. Our findings do not support the routine treatment of low-grade dAVFs