Effects of Vitamin-E, Morin, Rutin, Quercetin against Doxorubicin in Rabbits: A Hematological Study

ABSTRACT Vitamin-E and flavonoids are natural anti-oxidants present in the vegetable diet. The present study was conducted on New Zealand white rabbits aged between 3-6 months and averaging 1.5-3.0 kg in weight. Rabbits are divided into 5 groups of 6 in each; a 4-week feeding trail is used to evaluate the hematology of rabbits by feeding vitamin-E, flavonoids morin, rutin, and quercetin along with drug doxorubicin. Doxorubicin is a potential chemotherapeutic agent for the treatment of a variety of human malignancies. In the present study, the development of cardio toxicity was prevented by reducing oxidative stress using natural antioxidant vitamin E (50 IU/kg body weight) and flavonoids morin, rutin and quercetin. The four weeks treatment of flavonoids (20mg/kg body weight) were affectively controlled the oxidative stress induced cardiomyopathy in rabbits by doxorubicin (10mg/kg body weight) treatment for two days. The flavonoids regulated the hematological parameters at optimum levels. The hematological components of study included red blood cells (RBC), white blood cells WBC, Hemoglobin (Hb), lymphocytes (LYM), platelets (PLT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), basophiles (BAS), eosinophiles (EOI), monocytes (MON), and neutrophiles (NEU). Results showed that hematological parameters were affected (P<0.05) by experimental diets. The overall conclusion of this study is that the flavonoids can act as antioxidants and they can control the alteration in the hematological parameters. By this study, the authors suggests that the flavonoids are natural anti-oxidants, which can be used in the various treatments of cancers as supplement to reduce the oxidative stress produced by the drugs