Incidence et pouvoir pathogène expérimental d'une nouvelle espèce (streptococcus pseudopneumoniae)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Le pneumocoque (un redoutable pathogène)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Pneumopathies nosocomiales à acinetobacter baumannii (résultats du protocole de surveillance raisin-rea)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Streptocoques oraux du groupe anginosus (étude comparative des méthodes d'identification)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Evolution thérapeutique des cyclines

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Difficultés d'identification des streptocoques oraux du groupe mitis

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Allaitement maternel ou artificiel du nourrisson (besoins nutritionnels et risques infectieux)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Rôle des mycoplasmes dans la survenue des infertilités chez l'homme

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Thérapeutique des infections respiratoires (apport de la télithromycine)

STRASBOURG ILLKIRCH-Pharmacie (672182101) / SudocSudocFranceF

Rapid Aeromonas hydrophila identification by TaqMan PCR assay: comparison with a phenotypic method

International audienceAims: Aeromonas hydrophila is recognized as a human pathogen following wound exposure or ingestion of contaminated water and food. For rapid identification of this bacterium, a TaqMan-based real-time PCR assay has been developed. Methods and Results: Primers and probes that target specific sequences of the 16S rRNA gene and cytolytic enterotoxin gene (aerA) were combined in a duplex assay. Presence and size of PCR products were confirmed with microchannel fluidics electrophoresis analysis. After validation, using type strain CIP7614T DNA, the PCR assay was tested on 12 positive and negative controls. Twenty-one Aeromonas strains were isolated from environmental samples and were identified with biochemical tests as Aer. sobria, Aer. caviae and Aer. hydrophila. Only Aer. hydrophila strains tested positive by PCR assay. Conclusions: The PCR developed here was successfully applied for the identification of Aer. hydrophila from reference, clinical and environmental samples and showed a high discrimination between Aer. hydrophila and other Aeromonas species. Significance and Impact of the Study: This molecular method is convenient, rapid (2 center dot 5 h vs 24 h), specific to identify Aer. hydrophila and usable for diagnosis in medical and veterinary laboratories