Data underlying the research to a: Prognostic model based on six feature genes of intestinal flora subtypes predicts survival in colon cancer

A prognostic model was established based on six intestinal flora subtypes-associated prognostic genes（CSF1R, HLA-DOA, NOS3, HOXB4, PLA1A, and RPL3) in colon cancer. The expression of these six genes in human colonic cancer cell line SW480 and colonic epithelial cell line NCM460 were determined using RT-qPCR.</p

Data underlying the research to a: Prognostic model based on six feature genes of intestinal flora subtypes predicts survival in colon cancer

A prognostic model was established based on six intestinal flora subtypes-associated prognostic genes（CSF1R, HLA-DOA, NOS3, HOXB4, PLA1A, and RPL3) in colon cancer. The expression of these six genes in human colonic cancer cell line SW480 and colonic epithelial cell line NCM460 were determined using RT-qPCR.</p

Data underlying the research to a: Prognostic model based on six feature genes of intestinal flora subtypes predicts survival in colon cancer

A prognostic model was established based on six intestinal flora subtypes-associated prognostic genes（CSF1R, HLA-DOA, NOS3, HOXB4, PLA1A, and RPL3) in colon cancer. The expression of these six genes in human colonic cancer cell line SW480 and colonic epithelial cell line NCM460 were determined using RT-qPCR.</p

The epithelial polarity regulator LGALS9/galectin-9 induces fatal frustrated autophagy in KRAS mutant colon carcinoma that depends on elevated basal autophagic flux

Oncogenic mutation of KRAS (Kirsten rat sarcoma viral oncogene homolog) in colorectal cancer (CRC) confers resistance to both chemotherapy and EGFR (epidermal growth factor receptor)-targeted therapy. We uncovered that KRAS mutant (KRASmut) CRC is uniquely sensitive to treatment with recombinant LGALS9/Galectin-9 (rLGALS9), a recently established regulator of epithelial polarity. Upon treatment of CRC cells, rLGALS9 rapidly internalizes via early- and late-endosomes and accumulates in the lysosomal compartment. Treatment with rLGALS9 is accompanied by induction of frustrated autophagy in KRASmut CRC, but not in CRC with BRAF (B-Raf proto-oncogene, serine/threonine kinase) mutations (BRAFmut). In KRASmut CRC, rLGALS9 acts as a lysosomal inhibitor that inhibits autophagosome-lysosome fusion, leading to autophagosome accumulation, excessive lysosomal swelling and cell death. This antitumor activity of rLGALS9 directly correlates with elevated basal autophagic flux in KRASmut cancer cells. Thus, rLGALS9 has potent antitumor activity toward refractory KRASmut CRC cells that may be exploitable for therapeutic use