Anti-Bacterial activity of total flavonoids of Portulaca Oleracea L.

Flavanoids are uniquitous in photosynthesizing cells and are commonly found in fruit, vegetables, nuts, seeds, stems, flowers and wine. For centuries, preparations canting these compounds as the principal, physiologically active constituents have been isolated and used to treat human diseases. Portuleca olerace L is an important medicinal plant that has been used in traditional medicine. The present study was aimed to isolate the total flovanoids and to investigate an anti-bacterial activity of total flavonids extracted from aerial part of Portulaca olerace L. we have used five standard pathogenic bacterial strains like Pseudomonas aeruginosa, Salmonella typhimurium, Proteus mirabilis, Klebsiella pneumonia and Enterobacter aerogenes, among all the bacterial strains Salmonella typhimurium (14.33±0.2886) and Proteus mirabilis (17.16±0.0281) have shown maximum zone of inhibition for total flovanoids and remaining bacterial strains have shown moderate zone of inhibition when compared with control (20.66±0.2881). In case of bio-assay method Salmonella typhimurium was shown more sensitive by low turbidity of OD value (0.187)  indicating most significant result. The Minimum inhibitory Concentration (MIC) of the total flavonoids isolated from Portuleca olerace L was tested at the concentration ranging from undiluted sample to 10mg/ml. the minimum inhibition concentration (MIC) for the total flavonoids for all tested bacterial strains was >10mg/ml. Experimental results supports that these flavonoids have antibacterial properties which helps in the developing antibacterial agents in the form of drugs for the therapy of infectious diseases caused by these bacterial pathogens

Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.

BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml. RESULTS: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 Mg/ml were not cytotoxic and appears to be safe. CONCLUSIONS: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study