2 research outputs found
Thromboxane Modulates Endothelial Permeability
The study tests the role of thromboxane in modulating microvascular
permeability in vitro. Cultured monolayers of bovine
aortic endothelial cells were challenged with the thromboxane (Tx)
mimic U46619. This led to disassembly of actin microfilaments, cell
rounding, border retraction and interendotheHal gap formation.
Pretreatment with the Tx receptor antagonist SQ 29,548 prevented the
Tx mimic-induced cytoskeletal changes. The Tx mimic also altered
endothelial cell barrier function. Increased permeability was
indicated by the increased passage of labelled albumin across
monolayers cultured on microcarriers, relative to untreated
endothelial cells (p < 0.05). Furthermore, electron microscopy
of endothelial cells cultured on the basement membrane of human
placental amnion indicated increased permeability based on wide,
interendotheHal gap formation and transit of the tracer horseradish
peroxidase. Quantification of interendothelial gaps revealed an
eleven-fold increase with the Tx mimic relative to untreated
endothial cells (p < 0.05) and prevention by pretreatment with
the Tx receptor antagonist (p < 0.05). These data indicate that
Tx directly modulates the permeability of endothelial cell in
vitro