An IIoT-Device for Acquisition and Analysis of High-Frequency Data Processed by Artificial Intelligence

This publication presents the development of an Industrial-Internet-of-Things device. The device is capable of completing several tasks, such as the acquisition of high-frequency measurement data and evaluating data via machine learning methods in an artificial intelligence application. The installed measurement technology generates data which is comparable to data generated by costly laboratory equipment, meaning that it can be used as a low-budget and open-source alternative. A workflow method has been designed that promotes experimental work and simplifies the effort required to implement artificial intelligence solutions. At the end of this paper, the results of the experiment, which aimed to collect measurement data, extract suitable features, and train artificial intelligence models, are presented. Techniques from vibration analysis were used for feature extraction, and concepts for the extrapolation and enhancement of data sets were investigated. The test results have proven that the development is comparable with high-end laboratory equipment. The created application has demonstrated sufficient accuracy in predictions, and the designed process can be used for arbitrary, artificial intelligence-based rapid prototyping

VEGF triggers the activation of Cofilin and the Arp2/3 complex within the growth cone

A crucial neuronal structure for the development and regeneration of neuronal networks is the axonal growth cone. Affected by different guidance cues, it grows in a predetermined direction to reach its final destination. One of those cues is the vascular endothelial growth factor (VEGF), which was identified as a positive effector for growth cone movement. These positive effects are mainly mediated by a reorganization of the actin network. This study shows that VEGF triggers a tight colocalization of cofilin and the Arp2/3 complex to the actin cytoskeleton within chicken dorsal root ganglia (DRG). Live cell imaging after microinjection of GFP (green fluorescent protein)-cofilin and RFP (red fluorescent protein)-LifeAct revealed that both labeled proteins rapidly redistributed within growth cones, and showed a congruent distribution pattern after VEGF supplementation. Disruption of signaling upstream of cofilin via blocking LIM-kinase (LIMK) activity resulted in growth cones displaying regressive growth behavior. Microinjection of GFP-p16b (a subunit of the Arp2/3 complex) and RFP-LifeAct revealed that both proteins redistributed into lamellipodia of the growth cone within minutes after VEGF stimulation. Disruption of the signaling to the Arp2/3 complex in the presence of VEGF by inhibition of N-WASP (neuronal Wiskott–Aldrich–Scott protein) caused retraction of growth cones. Hence, cofilin and the Arp2/3 complex appear to be downstream effector proteins of VEGF signaling to the actin cytoskeleton of DRG growth cones. Our data suggest that VEGF simultaneously affects different pathways for signaling to the actin cytoskeleton, since activation of cofilin occurs via inhibition of LIMK, whereas activation of Arp2/3 is achieved by stimulation of N-WASP

Recent developments of common numerical methods and common experimental means within the framework of the large passenger aircraft programme

For the exploration and validation of the integration of efficient propulsion concepts and new technologies for future large passenger aircraft, it is necessary to establish suitable design, evaluation and measurement tools. Therefore, as one major activity of the Large Passenger Aircraft (LPA) Platform 1 of the Clean Sky 2 initiative, the so-called Cross-Capability Demonstrator (XDC), has been set up to develop and demonstrate powerful numerical and experimental methods for aerodynamic, aeroacoustics, and aeroelastic simulation and measurement tasks. The paper will give an overview of the activities to be performed within the XDC and present some of the latest achievements related to this demonstrator

Recent developments of common numerical methods and common experimental means within the framework of the large passenger aircraft programme

For the exploration and validation of the integration of efficient propulsion concepts and new technologies for future large passenger aircraft it is necessary to establish suitable design, evaluation and measurement tools. Therefore, as one major activity of the Large Passenger Aircraft (LPA) Platform 1 of the Clean Sky 2 initiative the so-called Cross-Capability-Demonstrator (XDC) has been set up to develop and demonstrate powerful numerical and experimental methods for aerodynamic, aeroacoustics and aeroelastic simulation and measurement tasks. The paper will give an overview of the activities to be performed within the XDC and present some of the latest achievements related to this demonstrator

Wnt11 is required for oriented migration of dermogenic progenitor cells from the dorsomedial lip of the avian dermomyotome

The embryonic origin of the dermis in vertebrates can be traced back to the dermomyotome of the somites, the lateral plate mesoderm and the neural crest. The dermal precursors directly overlying the neural tube display a unique dense arrangement and are the first to induce skin appendage formation in vertebrate embryos. These dermal precursor cells have been shown to derive from the dorsomedial lip of the dermomyotome (DML). Based on its expression pattern in the DML, $\it Wnt11$ is a candidate regulator of dorsal dermis formation. Using EGFP-based cell labelling and time-lapse imaging, we show that the $\it Wnt11$ expressing DML is the source of the dense dorsal dermis. Loss-of-function studies in chicken embryos show that $\it Wnt11$ is indeed essential for the formation of dense dermis competent to support cutaneous appendage formation. Our findings show that dermogenic progenitors cannot leave the DML to form dense dorsal dermis following $\it Wnt11$ silencing. No alterations were noticeable in the patterning or in the epithelial state of the dermomyotome including the DML. Furthermore, we show that $\it Wnt11$ expression is regulated in a manner similar to the previously described early dermal marker $\it cDermo-1$. The analysis of Wnt11mutant mice exhibits an underdeveloped dorsal dermis and strongly supports our gene silencing data in chicken embryos. We conclude that Wnt11 is required for dense dermis and subsequent cutaneous appendage formation, by influencing the cell fate decision of the cells in the DML