Acoustic cues to individuality in wild male adult African savannah elephants (Loxodonta africana).

The ability to recognize conspecifics plays a pivotal role in animal communication systems. It is especially important for establishing and maintaining associations among individuals of social, long-lived species, such as elephants. While research on female elephant sociality and communication is prevalent, until recently male elephants have been considered far less social than females. This resulted in a dearth of information about their communication and recognition abilities. With new knowledge about the intricacies of the male elephant social structure come questions regarding the communication basis that allows for social bonds to be established and maintained. By analyzing the acoustic parameters of social rumbles recorded over 1.5 years from wild, mature, male African savanna elephants (Loxodonta africana) we expand current knowledge about the information encoded within these vocalizations and their potential to facilitate individual recognition. We showed that social rumbles are individually distinct and stable over time and therefore provide an acoustic basis for individual recognition. Furthermore, our results revealed that different frequency parameters contribute to individual differences of these vocalizations

Acoustic cues to individuality in wild male adult African savannah elephants (Loxodonta africana)

The ability to recognize conspecifics plays a pivotal role in animal communication systems. It is especially important for establishing and maintaining associations among individuals of social, long-lived species, such as elephants. While research on female elephant sociality and communication is prevalent, until recently male elephants have been considered far less social than females. This resulted in a dearth of information about their communication and recognition abilities. With new knowledge about the intricacies of the male elephant social structure come questions regarding the communication basis that allows for social bonds to be established and maintained. By analyzing the acoustic parameters of social rumbles recorded over 1.5 years from wild, mature, male African savanna elephants (Loxodonta africana) we expand current knowledge about the information encoded within these vocalizations and their potential to facilitate individual recognition. We showed that social rumbles are individually distinct and stable over time and therefore provide an acoustic basis for individual recognition. Furthermore, our results revealed that different frequency parameters contribute to individual differences of these vocalizations

Mining morphometrics and age from past survey photographs

Abstract Background Researchers often document wildlife surveys using images. These images contain data that can be used to understand alterative research objectives, even years after they were originally captured. We have developed a method to measure age and morphology (body size measurements and tusk size) from survey image databases and future surveys, without the availability of a known subject distance or a scale in each image. African savanna elephants (Loxodonta africana) serve as an ideal model species to develop a non-invasive, image-based morphometric methodology: as handling these animals is particularly invasive and expensive, involving anaesthesia and because of their IUCN ‘vulnerable’ status. We compare in situ measurements, taken during collaring events, to tusk-to-body-size ratios, measured from the images. Results We provide evidence that relative morphological measurements, musth timing, and age of male African savanna elephants can accurately be obtained from a survey image database of over 30,000 images, taken over an 18-year period. Of the 11 tusk to body size ratios calculated, we recommend the use of two in particular for future measurement in African elephants to determine size and age: 1) tusk length to tusk diameter and 2) tusk length to body height. Conclusions We present a practical, non-invasive measure to estimate morphometrics, including both age and tusk size from photographs, which has conservation applications to the protection of elephants and is relevant to a range of other taxa

Mining morphometrics and age from past survey photographs

Background Researchers often document wildlife surveys using images. These images contain data that can be used to understand alterative research objectives, even years after they were originally captured. We have developed a method to measure age and morphology (body size measurements and tusk size) from survey image databases and future surveys, without the availability of a known subject distance or a scale in each image. African savanna elephants (Loxodonta africana) serve as an ideal model species to develop a non-invasive, image-based morphometric methodology: as handling these animals is particularly invasive and expensive, involving anaesthesia and because of their IUCN ‘vulnerable’ status. We compare in situ measurements, taken during collaring events, to tusk-to-body-size ratios, measured from the images. Results We provide evidence that relative morphological measurements, musth timing, and age of male African savanna elephants can accurately be obtained from a survey image database of over 30,000 images, taken over an 18-year period. Of the 11 tusk to body size ratios calculated, we recommend the use of two in particular for future measurement in African elephants to determine size and age: 1) tusk length to tusk diameter and 2) tusk length to body height. Conclusions We present a practical, non-invasive measure to estimate morphometrics, including both age and tusk size from photographs, which has conservation applications to the protection of elephants and is relevant to a range of other taxa

Parasite-associated mortality in a long-lived mammal: Variation with host age, sex, and reproduction.

Parasites can cause severe host morbidity and threaten survival. As parasites are generally aggregated within certain host demographics, they are likely to affect a small proportion of the entire population, with specific hosts being at particular risk. However, little is known as to whether increased host mortality from parasitic causes is experienced by specific host demographics. Outside of theoretical studies, there is a paucity of literature concerning dynamics of parasite-associated host mortality. Empirical evidence mainly focuses on short-lived hosts or model systems, with data lacking from long-lived wild or semi-wild vertebrate populations. We investigated parasite-associated mortality utilizing a multigenerational database of mortality, health, and reproductive data for over 4,000 semi-captive timber elephants (Elephas maximus), with known causes of death for mortality events. We determined variation in mortality according to a number of host traits that are commonly associated with variation in parasitism within mammals: age, sex, and reproductive investment in females. We found that potentially parasite-associated mortality varied significantly across elephant ages, with individuals at extremes of lifespan (young and old) at highest risk. Mortality probability was significantly higher for males across all ages. Female reproducers experienced a lower probability of potentially parasite-associated mortality than females who did not reproduce at any investigated time frame. Our results demonstrate increased potentially parasite-associated mortality within particular demographic groups. These groups (males, juveniles, elderly adults) have been identified in other studies as susceptible to parasitism, stressing the need for further work investigating links between infection and mortality. Furthermore, we show variation between reproductive and non-reproductive females, with mothers being less at risk of potentially parasite mortality than nonreproducers

Elephants born in the high stress season have faster reproductive ageing

Senescent declines in reproduction and survival are found across the tree of life, but little is known of the factors causing individual variation in reproductive ageing rates. One contributor may be variation in early developmental conditions, but only a few studies quantify the effects of early environment on reproductive ageing and none concern comparably long-lived species to humans. We determine the effects of ‘stressful’ birth conditions on lifetime reproduction in a large semi-captive population of Asian elephants (Elephas maximus). We categorise birth month into stressful vs. not-stressful periods based on longitudinal measures of glucocorticoid metabolites in reproductive-aged females, which peak during heavy workload and the start of the monsoon in June-August. Females born in these months exhibit faster reproductive senescence in adulthood and have significantly reduced lifetime reproductive success than their counterparts born at other times of year. Improving developmental conditions could therefore delay reproductive ageing in species as long-lived as humans

Parasite-associated mortality in a long-lived mammal: Variation with host age, sex, and reproduction

Parasites can cause severe host morbidity and threaten survival. As parasites are generally aggregated within certain host demographics, they are likely to affect a small proportion of the entire population, with specific hosts being at particular risk. However, little is known as to whether increased host mortality from parasitic causes is experienced by specific host demographics. Outside of theoretical studies, there is a paucity of literature concerning dynamics of parasite-associated host mortality. Empirical evidence mainly focuses on short-lived hosts or model systems, with data lacking from long-lived wild or semi-wild vertebrate populations. We investigated parasite-associated mortality utilizing a multigenerational database of mortality, health, and reproductive data for over 4,000 semi-captive timber elephants (Elephas maximus), with known causes of death for mortality events. We determined variation in mortality according to a number of host traits that are commonly associated with variation in parasitism within mammals: age, sex, and reproductive investment in females. We found that potentially parasite-associated mortality varied significantly across elephant ages, with individuals at extremes of lifespan (young and old) at highest risk. Mortality probability was significantly higher for males across all ages. Female reproducers experienced a lower probability of potentially parasite-associated mortality than females who did not reproduce at any investigated time frame. Our results demonstrate increased potentially parasite-associated mortality within particular demographic groups. These groups (males, juveniles, elderly adults) have been identified in other studies as susceptible to parasitism, stressing the need for further work investigating links between infection and mortality. Furthermore, we show variation between reproductive and non-reproductive females, with mothers being less at risk of potentially parasite mortality than nonreproducers.</p

Mendelian Randomisation Study of Childhood BMI and Early Menarche

To infer the causal association between childhood BMI and age at menarche, we performed a mendelian randomisation analysis using twelve established “BMI-increasing” genetic variants as an instrumental variable (IV) for higher BMI. In 8,156 women of European descent from the EPIC-Norfolk cohort, height was measured at age 39–77 years; age at menarche was self-recalled, as was body weight at age 20 years, and BMI at 20 was calculated as a proxy for childhood BMI. DNA was genotyped for twelve BMI-associated common variants (in/near FTO, MC4R, TMEM18, GNPDA2, KCTD15, NEGR1, BDNF, ETV5, MTCH2, SEC16B, FAIM2 and SH2B1), and for each individual a “BMI-increasing-allele-score” was calculated by summing the number of BMI-increasing alleles across all 12 loci. Using this BMI-increasing-allele-score as an instrumental variable for BMI, each 1 kg/m2 increase in childhood BMI was predicted to result in a 6.5% (95% CI: 4.6–8.5%) higher absolute risk of early menarche (before age 12 years). While mendelian randomisation analysis is dependent on a number of assumptions, our findings support a causal effect of BMI on early menarche and suggests that increasing prevalence of childhood obesity will lead to similar trends in the prevalence of early menarche

Mendelian Randomisation Study of Childhood BMI and Early Menarche.

To infer the causal association between childhood BMI and age at menarche, we performed a mendelian randomisation analysis using twelve established "BMI-increasing" genetic variants as an instrumental variable (IV) for higher BMI. In 8,156 women of European descent from the EPIC-Norfolk cohort, height was measured at age 39-77 years; age at menarche was self-recalled, as was body weight at age 20 years, and BMI at 20 was calculated as a proxy for childhood BMI. DNA was genotyped for twelve BMI-associated common variants (in/near FTO, MC4R, TMEM18, GNPDA2, KCTD15, NEGR1, BDNF, ETV5, MTCH2, SEC16B, FAIM2 and SH2B1), and for each individual a "BMI-increasing-allele-score" was calculated by summing the number of BMI-increasing alleles across all 12 loci. Using this BMI-increasing-allele-score as an instrumental variable for BMI, each 1 kg/m(2) increase in childhood BMI was predicted to result in a 6.5% (95% CI: 4.6-8.5%) higher absolute risk of early menarche (before age 12 years). While mendelian randomisation analysis is dependent on a number of assumptions, our findings support a causal effect of BMI on early menarche and suggests that increasing prevalence of childhood obesity will lead to similar trends in the prevalence of early menarche.Peer Reviewe

How Big Is It Really? Assessing the Efficacy of Indirect Estimates of Body Size in Asian Elephants.

Information on an organism's body size is pivotal in understanding its life history and fitness, as well as helping inform conservation measures. However, for many species, particularly large-bodied wild animals, taking accurate body size measurements can be a challenge. Various means to estimate body size have been employed, from more direct methods such as using photogrammetry to obtain height or length measurements, to indirect prediction of weight using other body morphometrics or even the size of dung boli. It is often unclear how accurate these measures are because they cannot be compared to objective measures. Here, we investigate how well existing estimation equations predict the actual body weight of Asian elephants Elephas maximus, using body measurements (height, chest girth, length, foot circumference and neck circumference) taken directly from a large population of semi-captive animals in Myanmar (n = 404). We then define new and better fitting formulas to predict body weight in Myanmar elephants from these readily available measures. We also investigate whether the important parameters height and chest girth can be estimated from photographs (n = 151). Our results show considerable variation in the ability of existing estimation equations to predict weight, and that the equations proposed in this paper predict weight better in almost all circumstances. We also find that measurements from standardised photographs reflect body height and chest girth after applying minor adjustments. Our results have implications for size estimation of large wild animals in the field, as well as for management in captive settings.VL received funding from the European Research Council and Leverhulme Trust for this study. HSM received a Natural Environmental Research Council PhD studentship, and post-doctoral funding from the Leverhulme Trust, Society in Science—Branco Weiss Fellowship and a Drapers' Company Junior Research Fellowship