6 research outputs found
Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)
Abdominal surgeries alter the integrity of the peritoneal layer and cause imbalances among immunological,
inflammatory and angiogenic mechanisms within the tissue. During laparoscopic procedures a protective
function of the peritoneal layer can be disturbed by the gas used to create a pneumoperitoneum. The aim of
this study was to characterize peritoneal tissue by means of differential scanning calorimetry (DSC) as a reference
for future investigations on the influence of surgical procedures on the physicochemical state of the peritoneum.
Thirty-seven patients participated in the study. Patients were divided into three groups according to the
type of surgery: group H — patients who underwent hernia repair; group Ch — patients who underwent laparoscopic
cholecystectomy; and group C — patients operated due to rectal cancer. It was observed that onset
temperature (To), denaturation temperature (Tm) and change of enthalpy (ΔH) during thermal denaturation of
peritoneal collagen in were significantly different for these three groups of patients. The mean values of onset
temperature (To) and denaturation temperature (Tm) in group H were significantly lower, while DH in this
group was significantly higher than in the two other groups (Ch and C). This preliminary study does not answer
whether the differences in collagen denaturation found in peritoneal tissue from different groups of patients
resulted from a different inherent state of the tissue, or from surgical procedures. However, the results suggest
that DSC is an appropriate method to study subtle changes in the physicochemical condition of the peritoneum
using small samples obtained during surgical procedures. (Folia Histochemica et Cytobiologica 2011; Vol. 49,
No. 4, pp. 700–705
DSC, FT-IR and NIR with Chemometric Assessment Using PCA and HCA for Estimation of the Chemical Stability of Oral Antidiabetic Drug Linagliptin in the Presence of Pharmaceutical Excipients
Pharmaceutical excipients should not interact with active substances, however, in practice, they sometimes do it, affecting the efficacy, stability and safety of drugs. Thus, interactions between active substances and excipients are not desirable. For this reason, two component mixtures of oral antidiabetic drug linagliptin (LINA) with four excipients of different reactivity, i.e., lactose (LAC), mannitol (MAN), magnesium stearate (MGS) and polyvinylpyrrolidone (PVP), were prepared in a solid state. A high temperature and a high humidity of 60 °C and 70% RH, respectively, were applied as stressors in order to accelerate the potential interactions between LINA and excipients. Differential scanning calorimetry (DSC) as well as Fourier transform infrared (FT-IR) and near infrared (NIR) spectroscopy were used to estimate the changes due to potential interactions. In addition, chemometric computation of the data with principal component analysis (PCA) and hierarchical cluster analysis (HCA) was applied to adequately interpret the findings. Of the excipients used in the present experiment, all of them were not inert in relation to LINA. Some of the interactions were shown without any stressing, whereas others were observed under high-temperature/high-humidity conditions. Thus, it could be concluded that selection of appropriate excipients for LINA is very important question to minimize its degradation, especially when new types of formulations with LINA are being developed and manufactured