Concomitant percutaneous coronary intervention and transcatheter aortic valve replacement for aortic stenosis complicated with acute STEMI: a case report and literature review

Aortic stenosis (AS) complicated with acute ST-segment elevation myocardial infarction (STEMI) is a life-threatening emergency with high mortality. A 75-year-old male patient attended the emergency department of Wuhan Asia Heart Hospital in December 2021 with chest pain for 2 days and exacerbation for 1 h. The electrocardiogram (ECG) indicated atrial fibrillation with rapid ventricular response and ST-segment depression. Echocardiography showed severe AS and mild/moderate aortic insufficiency. The patient refused coronary angiography and further invasive procedures and then requested discharge, but he had recurrent chest pain on the third day. The ECG showed an extensive anterior wall STEMI. During preoperative preparation, he suffered from cardiogenic shock (CS). Concomitant percutaneous coronary intervention (PCI) and transcatheter aortic valve replacement (TAVR) was performed, but he died of CS and multiple organ failure 4 days after surgery. Patients with AS and STEMI might be susceptible to CS during perioperative period of concomitant PCI and TAVR, which requires proactive prevention

Pre-gestational stress reduces the ratio of 5-HIAA to 5-HT and the expression of 5-HT1A receptor and serotonin transporter in the brain of foetal rat

<p>Abstract</p> <p>Background</p> <p>Many studies have found that stress before or during pregnancy is linked to an increased incidence of behavioural disorders in offspring. However, few studies have investigated hypothalamic-pituitary-adrenal (HPA) axis activity and the serotonergic system as a consequence of pregestational stress. In the present study, we investigated the effect of pre-gestational stress on HPA axis activity in maternal rats and their foetuses and examined whether changes in HPA axis activity of maternal rats produced functional changes in the serotonergic system in the brain of foetuses.</p> <p>Results</p> <p>We used the behavioural tests to assess the model of chronic unpredictable stress (CUS) in maternal rats. We found the activity in the open field and sucrose consumption was lower for rats with CUS than for the controls. Body weight but not brain weight was higher for control foetuses than those from the CUS group. Serum corticosterone and corticotrophin-releasing hormone levels were significantly higher for mothers with CUS before pregnancy and their foetuses than for the controls. Levels of 5-hydroxytryptamine (5-HT) were higher in the hippocampus and hypothalamus of foetuses in the CUS group than in the controls, and 5-hydroxyindoleacetic acid (5-HIAA) levels were lower in the hippocampus in foetuses in the CUS group than in the control group. Levels of 5-HIAA in the hypothalamus did not differ between foetuses in the CUS group and in the control group. The ratio of 5-HIAA to 5-HT was significantly lower for foetuses in the CUS group than in the control group. Levels of 5-HT1A receptor were significantly lower in the foetal hippocampus in the CUS group than in the control group, with no significant difference in the hypothalamus. The levels of serotonin transporter (SERT) were lower in both the foetal hippocampus and foetal hypothalamus in the CUS group than in the control group.</p> <p>Conclusions</p> <p>Our data demonstrate that pre-gestational stress alters HPA axis activity in maternal rats and their foetuses, which is associated with functional changes in 5-HT activity (5-HT, 5-HIAA and ratio of 5-HIAA to 5-HT), as well as the levels of the 5-HT1A receptor and SERT in the hippocampus and hypothalamus of foetuses.</p

A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters

A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron

A Global View of Cancer-Specific Transcript Variants by Subtractive Transcriptome-Wide Analysis

BACKGROUND: Alternative pre-mRNA splicing (AS) plays a central role in generating complex proteomes and influences development and disease. However, the regulation and etiology of AS in human tumorigenesis is not well understood. METHODOLOGY/PRINCIPAL FINDINGS: A Basic Local Alignment Search Tool database was constructed for the expressed sequence tags (ESTs) from all available databases of human cancer and normal tissues. An insertion or deletion in the alignment of EST/EST was used to identify alternatively spliced transcripts. Alignment of the ESTs with the genomic sequence was further used to confirm AS. Alternatively spliced transcripts in each tissue were then subtractively cross-screened to obtain tissue-specific variants. We systematically identified and characterized cancer/tissue-specific and alternatively spliced variants in the human genome based on a global view. We identified 15,093 cancer-specific variants of 9,989 genes from 27 types of human cancers and 14,376 normal tissue-specific variants of 7,240 genes from 35 normal tissues, which cover the main types of human tumors and normal tissues. Approximately 70% of these transcripts are novel. These data were integrated into a database HCSAS (http://202.114.72.39/database/human.html, pass:68756253). Moreover, we observed that the cancer-specific AS of both oncogenes and tumor suppressor genes are associated with specific cancer types. Cancer shows a preference in the selection of alternative splice-sites and utilization of alternative splicing types. CONCLUSIONS/SIGNIFICANCE: These features of human cancer, together with the discovery of huge numbers of novel splice forms for cancer-associated genes, suggest an important and global role of cancer-specific AS during human tumorigenesis. We advise the use of cancer-specific alternative splicing as a potential source of new diagnostic, prognostic, predictive, and therapeutic tools for human cancer. The global view of cancer-specific AS is not only useful for exploring the complexity of the cancer transcriptome but also widens the eyeshot of clinical research

Non-Contact Experiment Investigation of the Interaction between the Soil and Underground Granary Subjected to Water Buoyancy

The buoyancy of underwater can cause the underground granary to overall float, or even overturn, and the interaction between the soil and underground granary is the key to its stability. This paper introduces a non-contact experiment system utilizing the digital image correlation (DIC) technology and particle image velocity (PIV) technology, and its measurement accuracy is analyzed. Then, this system is employed to study the granary displacement and the soil deformation around the granary subjected to the buoyancy of water. Results show that with the increase of the degree of compaction of the soil around the granary, the floating water level increases by 10.77% and the vertical displacement decreases by 17%. When the soils around the granary are loose sands, the soil deformation range shows an obvious inverted triangle. When the soils are medium dense sands, the soil deformation zone concentrates at the junction of the conical granary bottom and granary wall. When the soils are dense sands, the disturbed range of the soil obviously reduces, and the soil deformation concentrates on both sides of the granary wall and is distributed symmetrically. Finally, taking the medium dense sands around the granary as an example, the reasons for the unstable failure of the granary subjected to buoyancy are discussed, assisted by the soil pressure theory of retaining wall. With the granary increasingly inclining, the soil deviating from the inclined direction of the granary loses its support, which drives the soils to reach the active limit state. The soil in the inclined direction of the granary is squeezed, resulting in passive soil pressure on the granary wall. The soil deformation increases continuously to a passive limit equilibrium state, and the soil continuously develops a sliding surface, resulting in the unstable failure of the granary. This research is expected to provide the technical guidance for the design and popularization of underground granaries

The Optimal Sine Pulse Frequency of Pulse Hydraulic Fracturing for Reservoir Stimulation

Pulse hydraulic fracturing (PHF) is a key technique for reservoir stimulation. PHF can well accelerate the rupture of rock. However, the supercharging mechanism of PHF is not fully understood. The main reason is that the pressure distribution and its variation, especially the peak pressure characteristics, are unclear inside the pipe and fissure. The present research focuses on the sine pulse applied at the inlet of a pipe or fracture to reveal the variation regularity of peak pressure with the pulse frequency, amplitude, pipe length, diameter and wave speed. First, the weakly compressible Navier–Stokes equations were developed to simulate the variation of fluid pressure. The computation codes were developed using the MacCormack method validated by the existing experimental data. Then, the sine pulse effect was studied inside the pipe and fissure. Last, a new frequency model was built to describe the relationship between the optimal pulse frequency, wave speed and pipe length. The results show that there is a family of frequencies at which the peak pressure of the endpoint can be significantly enhanced and that these frequencies are the optimal pulse frequency. It is found that the optimal pulse frequency depends on the pipe or fissure length and wave speed. At the optimal pulse frequency, the peak pressure at the endpoint can be increased by 100% or more, and the cavitation phenomenon occurs. However, the peak pressure decreases when with the decrease in the pipe diameter and fissure departure due to the friction drag effect of the wall. These new landmark findings are very important for the PHF technique. In addition, a new universal frequency model is built to predict the optimal sine pulse frequency. The present research shows the variation regularity of the fluid pressure inside the pipe and develops a sine frequency-controlled method, providing a potential guide for reservoir stimulation

The Optimal Sine Pulse Frequency of Pulse Hydraulic Fracturing for Reservoir Stimulation

Pulse hydraulic fracturing (PHF) is a key technique for reservoir stimulation. PHF can well accelerate the rupture of rock. However, the supercharging mechanism of PHF is not fully understood. The main reason is that the pressure distribution and its variation, especially the peak pressure characteristics, are unclear inside the pipe and fissure. The present research focuses on the sine pulse applied at the inlet of a pipe or fracture to reveal the variation regularity of peak pressure with the pulse frequency, amplitude, pipe length, diameter and wave speed. First, the weakly compressible Navier&ndash;Stokes equations were developed to simulate the variation of fluid pressure. The computation codes were developed using the MacCormack method validated by the existing experimental data. Then, the sine pulse effect was studied inside the pipe and fissure. Last, a new frequency model was built to describe the relationship between the optimal pulse frequency, wave speed and pipe length. The results show that there is a family of frequencies at which the peak pressure of the endpoint can be significantly enhanced and that these frequencies are the optimal pulse frequency. It is found that the optimal pulse frequency depends on the pipe or fissure length and wave speed. At the optimal pulse frequency, the peak pressure at the endpoint can be increased by 100% or more, and the cavitation phenomenon occurs. However, the peak pressure decreases when with the decrease in the pipe diameter and fissure departure due to the friction drag effect of the wall. These new landmark findings are very important for the PHF technique. In addition, a new universal frequency model is built to predict the optimal sine pulse frequency. The present research shows the variation regularity of the fluid pressure inside the pipe and develops a sine frequency-controlled method, providing a potential guide for reservoir stimulation