Radioisotopes and coastal research in the Great Barrier Reef

Radioisotopes are efficient tracers of coastal processes on various spatial and temporal scales. The isotopes of radon and radium are particularly useful tools to understand hydrological land-ocean interaction because (a) activities of these isotopes are elevated in groundwater by two to three orders of magnitude in comparison with seawater, and (b) these isotopes have half-lives similar to the time scales of coastal hydrological processes such as river and groundwater discharge to the ocean, as well as coastal ocean mixing (or residence) time. The application of these isotopes to studies of land-ocean interaction in the central Great Barrier Reef region (Townsville to Cooktown) is illustrated in three recent studies: (1) coastal mapping of radon on a regional scale improves the understanding of the spatial variability of river and groundwater fluxes to the Great Barrier Reef lagoon; (2) quantification of tidal water exchange between mangrove forests and creeks demonstrates the significant contributions this process makes to water flux and associated geochemical fluxes along tropical coastlines; (3) estimates of coastal water residence time contribute to the understanding of the fate of land-derived solutes in the Great Barrier Reef Lagoon. Concurrent mapping of 222Rn (half-life 3.8 days) and salinity allows an efficient qualitative assessment of land–ocean interaction on various spatial and temporal scales. From shore-parallel transects along the Central Great Barrier Reef coastline with a surface-towed and continuously recording multi-detector system, numerous locations of elevated radon activities can be identified as terrestrially-derived submarine groundwater discharge, riverine sources, and the recirculation of seawater through crustacean burrows in mangrove forests. Variations in the inverse relationship of 222Rn and salinity in different tropical wet seasons reveal ‘timing’ aspects of large-scale freshwater input during the tropical wet season into the lagoon. Subsequently, 222Rn was used together with radium isotopes to quantify the tidal water exchange between a mangrove forest on Hinchinbrook Island and the ocean. Significant export of these radio-nuclides from the forest into a tidal creek indicates continuous tidally-driven circulation through animal burrows in the forest. The forest floor is efficiently flushed, with water flux of about 30 L m-2 day-1 of forest floor, which is equivalent to about 10% of the total burrow volume in the forest per tidal cycle. This work illustrates the physical process which supports export of organic and inorganic matter from mangrove forests to the coastal zone. Importantly, annual average circulation fluxes through mangrove forest floors are of the same order as annual river discharge in the central GBR. Finally, an improved understanding of the fate of land-derived waters is of great importance to current discussions about water quality management in the Great Barrier Reef. The mixing of coastal waters is an important parameter influencing the health of these ecosystems. Time constants associated with the decay of four naturally-occurring isotopes of radium span large time scales; 224Ra, 223Ra, 228Ra and 226Ra have half-lives of 4 days, 11 days, 6 years and 1620 years respectively. The radium quartet has been used to determine time scales of mixing of near-shore water and deep ocean water. This study demonstrates that central GBR water within 20 km of the coast is flushed with outer lagoon water on a timescale of 18–45 days, with the flushing time increasing northward. This difference likely reflects the different reef matrix density in the two zones, affecting exchange with offshore Coral Sea water

Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of Apis mellifera (Insecta: Hymenoptera): structure, organization, and retrotransposable elements

As an accompanying manuscript to the release of the honey bee genome, we report the entire sequence of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) ribosomal RNA (rRNA)-encoding gene sequences (rDNA) and related internally and externally transcribed spacer regions of Apis mellifera (Insecta: Hymenoptera: Apocrita). Additionally, we predict secondary structures for the mature rRNA molecules based on comparative sequence analyses with other arthropod taxa and reference to recently published crystal structures of the ribosome. In general, the structures of honey bee rRNAs are in agreement with previously predicted rRNA models from other arthropods in core regions of the rRNA, with little additional expansion in non-conserved regions. Our multiple sequence alignments are made available on several public databases and provide a preliminary establishment of a global structural model of all rRNAs from the insects. Additionally, we provide conserved stretches of sequences flanking the rDNA cistrons that comprise the externally transcribed spacer regions (ETS) and part of the intergenic spacer region (IGS), including several repetitive motifs. Finally, we report the occurrence of retrotransposition in the nuclear large subunit rDNA, as R2 elements are present in the usual insertion points found in other arthropods. Interestingly, functional R1 elements usually present in the genomes of insects were not detected in the honey bee rRNA genes. The reverse transcriptase products of the R2 elements are deduced from their putative open reading frames and structurally aligned with those from another hymenopteran insect, the jewel wasp Nasonia (Pteromalidae). Stretches of conserved amino acids shared between Apis and Nasonia are illustrated and serve as potential sites for primer design, as target amplicons within these R2 elements may serve as novel phylogenetic markers for Hymenoptera. Given the impending completion of the sequencing of the Nasonia genome, we expect our report eventually to shed light on the evolution of the hymenopteran genome within higher insects, particularly regarding the relative maintenance of conserved rDNA genes, related variable spacer regions and retrotransposable elements

Rapid seawater circulation through animal burrows in mangrove forests: a significant source of saline groundwater to the tropical coastal ocean

A common approach for quantifying rates of submarine groundwater discharge (SGD) to the coastal ocean is to use geochemical tracers such as 222Rn and short lived radium isotopes, which are naturally enriched in groundwater relative to seawater and have well understood chemistries within the marine environment. They occur in both fresh (continental) and saline (marine) groundwaters and thus the water source is often ambiguous. Here, we present a detailed investigation into the tidal circulation of seawater through animal burrows using 222Rn and isotopes of radium in the Coral Creek mangrove forest, Hinchinbrook Island, Queensland, Australia. The study was conducted at the end of the dry season in a creek with no freshwater inputs. Significant export of radionuclides and salt from the forest into the creek indicates continuous tidally driven circulation through the burrows. Results demonstrate that the forest sediment is efficiently flushed, with a water flux of about 30 L/m2/ day of forest floor, which is equivalent to flushing about 10% of the total burrow volume per tidal cycle. Importantly, annual average circulation flux through mangrove forest floors are of the same order as annual river discharge in the central GBR. However, unlike the river discharge, the tidal circulation should be relatively stable throughout the year. This work documents the importance of animal burrows in maintaining productive sediments in these systems, and illustrates the physical process that supports large exports of organic and inorganic matter from mangrove forests to the coastal zone. It also illustrates the importance of considering saline groundwater sources when interpreting SGD radionuclide tracers in the coastal ocean

Crossmodal Congruency Benefits For Tactile And Visual Signaling

We conducted an experiment in which tactile messages were created based on five common military arm and hand signals. We compared response times and accuracy rates of novice individuals responding to visual and tactile representations of these messages, which were displayed either alone or in congruent or incongruent combinations. Analyses were conducted on trials where tactile and visual signals messages were presented either individually or concurrently. Results indicated beneficial effects for concurrent, congruent message presentations with both modalities showing a superior response time and improved accuracy when compared to individual presentations in either modality. These results confirm the promise for tactile messages to augment visual messaging in challenging and stressful environments where visual messaging may not always be possible

Radioisotopes and coastal research in the Great Barrier\ud Reef

Radioisotopes are efficient tracers of coastal processes on various spatial and temporal scales. The isotopes of radon and radium are particularly useful tools to understand hydrological land-ocean interaction because (a) activities of these isotopes are elevated in groundwater by two to three orders of magnitude in comparison with seawater, and (b) these isotopes have half-lives similar to the time scales of coastal hydrological processes such as river and groundwater discharge to the ocean, as well as coastal ocean mixing (or residence) time. \ud \ud The application of these isotopes to studies of land-ocean interaction in the central Great Barrier Reef region (Townsville to Cooktown) is illustrated in three recent studies: (1) coastal mapping of radon on a regional scale improves the understanding of the spatial variability of river and groundwater fluxes to the Great Barrier Reef lagoon; (2) quantification of tidal water exchange between mangrove forests and creeks demonstrates the significant contributions this process makes to water flux and associated geochemical fluxes along tropical coastlines; (3) estimates of coastal water residence time contribute to the understanding of the fate of land-derived solutes in the Great Barrier Reef Lagoon. \ud \ud Concurrent mapping of 222Rn (half-life 3.8 days) and salinity allows an efficient qualitative assessment of land–ocean interaction on various spatial and temporal scales. From shore-parallel transects along the Central Great Barrier Reef coastline with a surface-towed and continuously recording multi-detector system, numerous locations of elevated radon activities can be identified as terrestrially-derived submarine groundwater discharge, riverine sources, and the recirculation of seawater through crustacean burrows in mangrove forests. Variations in the inverse relationship of 222Rn and salinity in different tropical wet seasons reveal ‘timing’ aspects of large-scale freshwater input during the tropical wet season into the lagoon. \ud \ud Subsequently, 222Rn was used together with radium isotopes to quantify the tidal water exchange between a mangrove forest on Hinchinbrook Island and the ocean. Significant export of these radio-nuclides from the forest into a tidal creek indicates continuous tidally-driven circulation through animal burrows in the forest. The forest floor is efficiently flushed, with water flux of about 30 L m-2 day-1 of forest floor, which is equivalent to about 10% of the total burrow volume in the forest per tidal cycle. This work illustrates the physical process which supports export of organic and inorganic matter from mangrove forests to the coastal zone. Importantly, annual average circulation fluxes through mangrove forest floors are of the same order as annual river discharge in the central GBR. \ud \ud Finally, an improved understanding of the fate of land-derived waters is of great importance to current discussions about water quality management in the Great Barrier Reef. The mixing of coastal waters is an important parameter influencing the health of these ecosystems. Time constants associated with the decay of four naturally-occurring isotopes of radium span large time scales; 224Ra, 223Ra, 228Ra and 226Ra have half-lives of 4 days, 11 days, 6 years and 1620 years respectively. The radium quartet has been used to determine time scales of mixing of near-shore water and deep ocean water. This study demonstrates that central GBR water within 20 km of the coast is flushed with outer lagoon water on a timescale of 18–45 days, with the flushing time increasing northward. This difference likely reflects the different reef matrix density in the two zones, affecting exchange with offshore Coral Sea water

Improving awareness of automated actions using an interactive event timeline

Digital tabletops provide an opportunity for automating complex tasks in collaborative domains involving planning and decision-making, such as strategic simulation in command and control. However, when automation leads to modification of the system's state, users may fail to understand how or why the state has changed, resulting in lower situation awareness and incorrect or suboptimal decisions. We present the design of an interactive event timeline that aims to improve situation awareness in tabletop systems that use automation. Our timeline enables exploration and analysis of automated system actions in a collaborative environment. We discuss two factors in the design of the timeline: the ownership of the timeline in multi-user situations and the location of the detailed visual feedback resulting from interaction with the timeline. We use a collaborative digital tabletop board game to illustrate this design concept.Ye