Characterization of tumour cell aggregation promoting factor from rat ascites hepatoma cells: Separation of two factors with different antigenic property.

The previously described glycoprotein that promotes tumour cell aggregation, derived from rat ascites hepatoma cells and capable of partial purification by chromatography, was found to be a mixture of 2 factors with different antigenic property. One was not absorbed by immunoadsorbent chromatography with anti-rat serum antibody and the other was. The action of the unabsorbed factor was clearly more potent than that of the absorbed factor. Both the factors were found in the serum of tumour bearing rats and the action of the unabsorbed factor was also more potent than that of the absorbed factor; its amount increased with time after i.p. inoculation of the cells. The serum of healthy rats contained the absorbed factor but not the unabsorbed factor. It was thus assumed that the unabsorbed factor was associated with the hepatoma cell surface itself and released into the serum, while the absorbed factor was associated with serum protein coating the cell

Contribution of the transport sector to climate change mitigation: Insights from a global passenger transport model coupled with a computable general equilibrium model

A passenger transport model, Asia-pacific Integrated Model (AIM)/Transport, incorporating travelers’ mode of choice and transport technological details was developed in this study. This AIM/Transport was coupled with the AIM/Computable General Equilibrium (AIM/CGE) to capture interactive mechanisms between the transport sector, energy consumption, greenhouse gas (GHG) emissions, and the macro-economy. This paper presents the model structure and mathematical formulation of AIM/Transport, and explains how it was integrated with the CGE model by an iterative algorithm, taking into consideration the feedback between AIM/Transport and AIM/CGE. A numerical simulation proved that the integration of AIM/CGE and AIM/Transport can achieve a convergence after 13 iterations. A business-as-usual (BaU) scenario and a mitigation scenario were created to test the robustness of the model integration and how the mitigation potential and cost would be modified by coupling AIM/Transport. The key finding was that the carbon price and mitigation cost were modified with the coupled CGE-Transport model

Tuning of magnetic quantum criticality in artificial Kondo superlattice CeRhIn5/YbRhIn5

The effects of reduced dimensions and the interfaces on antiferromagnetic quantum criticality are studied in epitaxial Kondo superlattices, with alternating $n$ layers of heavy-fermion antiferromagnet CeRhIn$_5$ and 7 layers of normal metal YbRhIn$_5$. As $n$ is reduced, the Kondo coherence temperature is suppressed due to the reduction of effective Kondo screening. The N\'{e}el temperature is gradually suppressed as $n$ decreases and the quasiparticle mass is strongly enhanced, implying dimensional control toward quantum criticality. Magnetotransport measurements reveal that a quantum critical point is reached for $n=3$ superlattice by applying small magnetic fields. Remarkably, the anisotropy of the quantum critical field is opposite to the expectations from the magnetic susceptibility in bulk CeRhIn$_5$, suggesting that the Rashba spin-orbit interaction arising from the inversion symmetry breaking at the interface plays a key role for tuning the quantum criticality in the two-dimensional Kondo lattice.Comment: Main text: 5 pages, 4 figures; Supplemental material:6 pages, 3 figures. Accepted for publication in Physical Review Letter

Atomic force microscopy sees nucleosome positioning and histone H1-induced compaction in reconstituted chromatin

AbstractWe addressed the question of how nuclear histones and DNA interact and form a nucleosome structure by applying atomic force microscopy to an in vitro reconstituted chromatin system. The molecular images obtained by atomic force microscopy demonstrated that oligonucleosomes reconstituted with purified core histones and DNA yielded a ‘beads on a string’ structure with each nucleosome trapping 158±27 bp DNA. When dinucleosomes were assembled on a DNA fragment containing two tandem repeats of the positioning sequence of the Xenopus 5S RNA gene, two nucleosomes were located around each positioning sequence. The spacing of the nucleosomes fluctuated in the absence of salt and the nucleosomes were stabilized around the range of the positioning signals in the presence of 50 mM NaCl. An addition of histone H1 to the system resulted in a tight compaction of the dinucleosomal structure