The Solution of the Austrian Public Debt in the 21. Century

The theses deals with the austrian public debt. The theoretical part gives a view into basic terms and problematic of public finance. The theoretical terms are applicated on the probletic of public finace of Austria. There is a comparison of accomplishment of nominal convergence criteria within the members of the European Union. The history of austrian economic policy since the 20. century is described. The theses then deals with the prediction of the economic development and growth. It is described, which economic impacts on austrian public finace this could have in the following period

The Solution of the Austrian Public Debt in the 21. Century

Práce se zabývá veřejným zadlužením Rakouska. V teoretická části je podáno základní vysvětlení pojmů a uvedení do problematiky veřejných financí. Praktická část se zabývá vývojem plnění nominálních konvergenčních kritérií veřejných deficitů a celkového veřejného dluhu. Je nastíněn vývoj hospodářské politiky, která k vedla k danému vývoji veřejného zadlužení. Dále se práce zabývá predikcí budoucího hoispodářského vývoje a s ním související dopady na fiskální politiku Rakouska a vývoj veřejného zadlužení v následujících letech.The theses deals with the austrian public debt. The theoretical part gives a view into basic terms and problematic of public finance. The theoretical terms are applicated on the probletic of public finace of Austria. There is a comparison of accomplishment of nominal convergence criteria within the members of the European Union. The history of austrian economic policy since the 20. century is described. The theses then deals with the prediction of the economic development and growth. It is described, which economic impacts on austrian public finace this could have in the following period

CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy

Objective. To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto’s thyroiditis (HT). Patients and Methods. The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT (controls) who underwent a fine needle aspiration biopsy (FNAB). Expression of chemokine receptors CXCR3, CCR5, and CRTH2 was assessed by flow cytometry in lymphocytes from FNAB samples and from peripheral blood. Results. The percentage of CRTH2+ lymphocytes was higher in nodules with HT in comparison with controls, both in FNAB samples (13.95 versus 6.7%, p=0.008) and in peripheral blood (6.7 versus 5.13%, p=0.047), and positively correlated with serum antibodies to thyroid peroxidase (r=0.243; p=0.026) and negatively correlated with thyroid volume (r=-0.346; p=0.008). Lymphocytes from neoplastic nodules showed a higher expression of both CXCR3 and CCR5 than those from hyperplastic ones. Conclusion. Flow cytometry performed in FNAB samples may serve as a good tool in investigation of intrathyroidal expression of immunological parameters. In our study, the CRTH2 expression on thyroid-infiltrating lymphocytes as well as on lymphocytes from peripheral blood was increased in HT as compared to controls

Development of Recombinant <i>Lactococcus lactis</i> Displaying Albumin-Binding Domain Variants against Shiga Toxin 1 B Subunit - Fig 7

<p>(A) SDS PAGE analysis of lysates of <i>L</i>. <i>lacti</i>s cells expressing S1B22, S1B26, ABDwt and H6-ABDwt (ABDwtH), all in fusion with Usp45 secretion signal and the LysM-containing cA domain, and stained with Coomassie brilliant blue. ABD fusion proteins are high-lighted with arrows. (B) Flow cytometric analysis of ABD surface display, detection with FITC-conjugated human serum albumin. The MFI value of ABDwt was compared with that of the control using Student’s t test. *** p<0.001. Cont.: control containing empty plasmid pNZ8148.</p

Influence of S1B binders on Stx1B transport into HeLa cells.

<p>A, B: Flow cytometric analysis of HeLa cells demonstrating shift in fluorescence intensity (A) and mean fluorescence intensity (MFI; B) upon 1h incubation of HeLa cells with mixtures of S1B22, S1B26 or ABDwt (all in fusion with TolA-Avi and H6) and Alexa Fluor 488-labeled Stx1B. Cont: unstained HeLa cells. Stx1B: HeLa cells incubated with Stx1B-Alexa Fluor 488 alone. C, D: Fluorescence microscopy images of HeLa cells incubated with Alexa Fluor 488 labelled Stx1B (green) with or without pre-incubation with S1B22 and S1B26. DAPI staining (blue) was used to label nuclei. Cells were stained either with PKH26 membrane labeling dye (red; C) and Golgi apparatus was detected with mouse monoclonal Golgin-97 antibody and secondary polyclonal goat anti-mouse antibody conjugated with Alexa Fluor 633 (purple; D). Bars = 10 μm.</p

SPR analysis of binding of S1B22, S1B26 and ABDwt in fusion with TolA-Avi and H6 at 1 μM concentration to immobilized recombinant Stx1B.

<p>SPR analysis of binding of S1B22, S1B26 and ABDwt in fusion with TolA-Avi and H6 at 1 μM concentration to immobilized recombinant Stx1B.</p

Sequence similarity analysis (A) and binding affinity (B) of 17 S1B binders selected after five rounds of ribosome display.

<p>A: The sequence of the parental ABD wild-type domain was used as a root of the tree and is highlighted as a square, while S1B variants selected for more detailed analysis are highlighted as circles. B: S1B binders-containing cell lysates were incubated with immobilized Stx1B (grey bars) or BSA (white bars) and detected with HRP-conjugated streptavidin. Error bars denote standard deviations.</p

Flow cytometric (A) and whole-cell ELISA (B) analyses of binding of recombinant Stx1B by <i>L</i>. <i>lactis</i> cells displaying S1B variants or ABDwt on their surface.

<p>(A) Alexa488-conjugated Stx1B was used for detection. MFI: Mean fluorescence intensity. (B) Mouse antiStx1B antibody and HRP-conjugated anti mouse antibody were used for detection of Stx1B. A₄₅₀: Absorbance at 450 nm. Vertical bars denote standard deviation. MFI or A₄₅₀ values of S1B binders were compared to those of the ABDwt control using Student’s t test. *: p<0.05, ** p<0.01, *** p<0.001.</p

Strains, plasmids, gene and primers used in this study.

<p>Strains, plasmids, gene and primers used in this study.</p