Influence of Milk Thistle (Silybum marianum) Seed Cakes on Biochemical Values of Equine Plasma Subjected to Physical Exertion

Veterinarians can recommend milk thistle for the treatment of equine liver disease and laminitis. Milk thistle seed cakes were fed in the range of normal feed doses in this study. The milk thistle seed cakes were fed (twice a day) to the experimental group of the horses (n = 5) and biochemical blood markers (TP, Albumin, ALT (alanine transaminase), AST (aspartate transaminase), ALP (alkaline phosphatase), GGT (gamma-glutamyltransferase), Bilirubin, Cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), TAG (triacylglycerol), BHB (beta-hydroxybutyric acid), NEFA (non-esterified fatty acids), creatine kinase, creatinine, Urea, GSH-Px (glutathione peroxidase), TAS (total antioxidant status), lactate, glucose, cortisol, Ca, Pi) were monitored. The control group of horses (n = 5), bred and trained in the same conditions, was used for comparison. The control group received the entire feed dose as accepted by the horses in the experimental group before the beginning of the experiment. The aim was to find out whether the preparation of milk thistle seed cakes could have positive effects on the health of the horses. All ten horses received one feeding form before the beginning of the experimental monitoring. All horses were exposed to heavy physical exercise (regular combined driving training) after 56 days of milk thistle seed cakes feeding (up to 400 g/day). Three blood samples were taken (before physical exercise; about 15 min and 60 min after physical exercise). Significant differences (p < 0.05) were detected in the values of AST, NEFA, cortisol and Pi in the experimental group. The exercise effect was detected in the values of albumin, lactate, cortisol, NEFA, and calcium. Our results suggest that the feeding of milk thistle seed cakes could have a positive effect on the health of the horses

Influence of Milk Thistle (<i>Silybum marianum</i>) Seed Cakes on Biochemical Values of Equine Plasma Subjected to Physical Exertion

Veterinarians can recommend milk thistle for the treatment of equine liver disease and laminitis. Milk thistle seed cakes were fed in the range of normal feed doses in this study. The milk thistle seed cakes were fed (twice a day) to the experimental group of the horses (n = 5) and biochemical blood markers (TP, Albumin, ALT (alanine transaminase), AST (aspartate transaminase), ALP (alkaline phosphatase), GGT (gamma-glutamyltransferase), Bilirubin, Cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), TAG (triacylglycerol), BHB (beta-hydroxybutyric acid), NEFA (non-esterified fatty acids), creatine kinase, creatinine, Urea, GSH-Px (glutathione peroxidase), TAS (total antioxidant status), lactate, glucose, cortisol, Ca, Pi) were monitored. The control group of horses (n = 5), bred and trained in the same conditions, was used for comparison. The control group received the entire feed dose as accepted by the horses in the experimental group before the beginning of the experiment. The aim was to find out whether the preparation of milk thistle seed cakes could have positive effects on the health of the horses. All ten horses received one feeding form before the beginning of the experimental monitoring. All horses were exposed to heavy physical exercise (regular combined driving training) after 56 days of milk thistle seed cakes feeding (up to 400 g/day). Three blood samples were taken (before physical exercise; about 15 min and 60 min after physical exercise). Significant differences (p < 0.05) were detected in the values of AST, NEFA, cortisol and Pi in the experimental group. The exercise effect was detected in the values of albumin, lactate, cortisol, NEFA, and calcium. Our results suggest that the feeding of milk thistle seed cakes could have a positive effect on the health of the horses

Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses

Milk thistle seeds contain a mixture of flavonoids known as silymarin, which consists of silybin, isosilybin, silychristine, and silydianin. Until now, there has been no evidence of monitoring the digestibility of silymarin complex in horses. The aim of the research was to evaluate the digestibility of silymarin complex and the effect of nutrient digestibility in horses. Different daily feed doses (FD) of milk thistle expeller (0 g, 100 g, 200 g, 400 g, 700 g) were administered to five mares kept under the same conditions and at the same feed rations. Digestibility of silymarin complex was monitored by HPLC-UV. Digestible energy (DE), crude protein, crude fat, crude fiber, nitrogen-free extract (NFE), crude ash, calcium (Ca), and phosphorus (P) were determined according ISO/IEC 17025:2017. The biochemical profile of blood plasma (total protein, albumin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), bilirubin, total cholesterol, HDL cholesterol, LDL cholesterol, triacyl glyceride (TAG), non-esterified fatty acid (NEFA), creatine kinase (CK), creatinine, urea, glutathione peroxidase (GSH-Px), total antioxidant status (TAS), glucose, calcium, and inorganic phosphate) was investigated. Moreover, the flavonolignans of the silymarin complex in plasma were detected. Statistically significant differences (p ≤ 0.05) were found between daily doses of milk thistle expellers in digestibilities. Our findings showed the digestibility of flavonolignans increased with the daily dose and then stagnated with the dose of milk thistle seed cakes at 700 g/day