The Cell Regulation Mechanism of Neurovascular Unit

Ischemic cerebrovascular disease is one of the three deadly diseases. It is characterised by high mortality and high morbidity. Because of no effective treatments of recombinant tissue plasminogen activator (rt-PA) and neuroprotectant, there are more and more research focus on neurovascular unit (NVU), which is composed of brain microvascular endothelial cells (BMECs), neuron, astrocyte(AS) and so on. Cell-cell signaling and coupling between these different compartments form the basis for normal function and repair of brain injury. In this mini-review, we will describe the relationship of CMECs, neuron and AS

1082 Free-breathing single-shot DENSE myocardial strain imaging using deformable registration

Free-breathing scans are often desirable in patients who find breath-holding difficult. We present a new approach for free-breathing myocardial strain imaging with displacement-encoding (DENSE) [1]. It acquires images with a single-shot sequence and removes respiratory motion using deformable registration

Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)

Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H. cumingii